A Glimpse into the Proteome of Phototrophic Bacterium Rhodobacter capsulatus
A first glimpse into the proteome of Rhodobacter capsulatus revealed more than 450 (with over 210 cytoplasmic and 185 extracytoplasmic known as well as 55 unknown) proteins that are identified with high degree of confidence using nLC–MS/MS analyses. The accumulated data provide a solid platform for ongoing efforts to establish the proteome of this species and the cellular locations of its constituents. They also indicate that at least 40 of the identified proteins, which were annotated in genome databases as unknown hypothetical proteins, correspond to predicted translation products that are indeed present in cells under the growth conditions used in this work. In addition, matching the identification labels of the proteins reported between the two available R. capsulatus genome databases (ERGO-light with RRCxxxxx and NT05 with NT05RCxxxx numbers) indicated that 11 such proteins are listed only in the latter database.
KeywordsMajor Metabolic Pathway Acclaim PepMap100 Tryptic Peptide Mixture Comprehensive Proteome Analysis Predict Translation Product
This work is supported by grants from DOE ER9120053 and NIH GM38237 to F.D.
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