Abstract
Objectives: To study the association between the mean telomere length (MTL) of human umbilical endothelial cells (HUVEC) and their sensitivity to apoptosis.
Methods: Apoptosis of HUVEC was induced by using free hydroxyl radicals. The rate of apoptosis was determined and mean telomere length of HUVEC that were cultured for 1 or 3 months were measured by Southern Blot.
Results: At 0.2 mmol/l FeSO4/0.0001 mmol/l H2O2 free radical concentration, the apoptosis rate was 8.0 and 17.5% and MTL was 4.66 ± 0.05 and 3.40 ± 0.46 kb for HUVEC cultured for 1 and 3 months, respectively. At 0.2 mmol/l FeSO4/0.005 mmol/l H2O2, apoptosis rates were 17.4 and 36.0% and MTL were 3.67 ± 0.06 and 2.90 ± 0.20 kb for HUVEC cultured for 1 and for 3 months, respectively. Control HUVEC had apoptosis rates of 0.5 and 1.0% and MTL of 5.43 ± 0.45 and 4.57 ± 0.21 kb for 1 and 3 months, respectively. The MTL and the apoptosis rates in the treatment groups differed significantly from the controls (p <0.05).
Conclusions: HUVEC with less culture time or short telomere were sensitive to oxidation stress. Oxidation stress also can enhance the shortening of telomere length.
Ji Zhang, Chen Hui, and Liu Su are co-first authors.
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References
Bodnar AG, Ouellette M, Frolkis M et al (1998) Extension of life-span by introduction of telomerase into normal human cells. Science 279(5349):349–352
Brouilette SW, Whittaker A, Stevens SE et al (2008) Telomere length is shorter in healthy offspring of subjects with coronary artery disease: support for the telomere hypothesis. Heart 94(4):422–425
Cawthon RM, Smith KR, O’Brien E et al (2003) Association between telomere length in blood and mortality in people aged 60 years or older. Lancet 361(9355):393–395
De Meyer T, Rietzschel ER, De Buyzere ML et al (2008) Studying telomeres in a population based study. Front Biosci 13:2960–2970
Gilley D, Herbert BS, Huda N et al (2008) Factors impacting human telomere homeostasis and age-related disease. Mech Ageing Dev 129(1–2):27–34
Goytisolo F-A, Samper E, Martin-Caballero J et al (2000) Short telomeres result in organismal hypersensitivity to ionizing radiation in mammals. J Exp Med 192(11):1625–1636
Harley CB, Futcher AB, Greider CW (1990) Telomeres shorten during ageing of human fibroblasts. Nature 345(6274):458–460
Horvitz HR, Herskowitz I (1992) Mechanisms of asymmetric cell division: two Bs or not two Bs, that is the question. Cell 68(2):237–255
Huda N, Tanaka H, Herbert BS (2007) Shared environmental factors associated with telomere length maintenance in elderly male twins. Aging Cell 6(5):709–713
Kurz DJ, Kloeckener-Gruissem B, Akhmedov A et al (2006) Degenerative aortic valve stenosis, but not coronary disease, is associated with shorter telomere length in the elderly. Arterioscler Thromb Vasc Biol 26(6):e114–e117
Melk A, Ramassar V, Helms LM et al (2000) Telomere shortening in kidneys with age. J Am Soc Nephrol 11(3):444–453
Morrison SJ, Shah NM, Anderson DJ et al (1997) Regulatory mechanisms in stem cell biology. Cell 88(3):287–298
Pendergrass WR, Penn PE, Li J et al (2001) Age-related telomere shortening occurs in lens epithelium from old rats and is slowed by caloric restriction. Exp Eye Res 73(2):221–228
Starr JM, Shiels PG, Harris SE et al (2008) Oxidative stress, telomere length and biomarkers of physical aging in a cohort aged 79 years from the 1932 Scottish Mental Survey. Mech Ageing 129(12):745–751
Thomas P, O’Callaghan NJ, Fenech M (2008) Telomere length in white blood cells, buccal cells and brain tissue and its variation with ageing and Alzheimer’s disease. Mech Ageing Dev 129(4):183–190
Toussaint O, Medrano EE, von Zglinicki T (2000) Cellular and molecular mechanisms of stress-induced premature senescence (SIPS) of human diploidfibroblasts and melanocytes. Exp Gerontol 35(8):927–945
van Steensel B, Smogorzewska A, de Lange T (1998) TRF2 protects human telomeres from end-to-end fusions. Cell 92(3):401–413
Vaziri H, Schachter F, Uchida I et al (1993) Loss of telomeric DNA during aging of normal and trisomy 21 human lymphocytes. Am J Hum Genet 52:661–667
Verzola D, Gandolfo MT, Gaetani G et al (2008) Accelerated senescence in the kidneys of patients with type 2 diabetic nephropathy. Am J Physiol Renal Physiol 295(5):F1563–F1573
Yamazaki Y, Matsunaga H, Nishikawa M et al (2007) Senescence in cultured trabecular meshwork cells. Br J Ophthalmol 91(6):808–811
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Zhang, J., Hui, C., Su, L., Xiaoyun, W., Xi, H., Yingchuan, F. (2010). The Association Between Telomere Length and Sensitivity to Apoptosis of HUVEC. In: Anderson, R., Hollyfield, J., LaVail, M. (eds) Retinal Degenerative Diseases. Advances in Experimental Medicine and Biology, vol 664. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-1399-9_6
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