Abstract
Objective: To study the effect of hesperidin on expression of inducible nitric oxide synthase (iNOS) in cultured rabbit retinal pigment epithelial (RPE) cells under the condition of high glucose in vitro.
Method: Hesperidin was extracted from Pericarpium Citri Reticulatae by ultrasound and ethanol precipitation and was detected qualitatively by high performance liquid chromatogram. The third to fifth primary cultured rabbit RPE were selected. The cells were divided into 6 groups including the control group cultured in DMEM, the model group cultured in DMEM containing 33 mmol/L glucose without any drug and four experimental groups which were exposed to hesperidin at the concentration of 10, 20, 40 and 80 mg/L at 37°C under 5% CO2 for 2 h and then cultured in DMEM containing 33 mmol/L glucose. The proliferation of RPE was measured by the MTT assay. The levels of NO produced were measured by spectrophotometry. The changes of iNOS expressed in RPE cells were determined with immunohistochemistry.
Results: The growth rate of RPE cells was associated with the concentration of hesperidin. NO production induced by high glucose was significantly inhibited by hesperidin. iNOS expression in hesperidin-treated group was decreased compared with the control group (p <0.001).
Conclusion: Hesperidin can increase the proliferation of rabbit RPE cells, and inhibit the level of NO and iNOS expression, so hesperidin can protect rabbit RPE cells.
Supplied by: Department of Education of Jiangxi Province (NO.GJJ08401) and Gannan Medical College (NO.2006070). Brief introduction of author: Luo Xiaoting, female, born in 1976, is an associated professor. Her research field is mainly in molecular pharmacology. Tel: 0797-8657990; Fax: 0797-8200037; E-mail: xtluo76@yahoo.com.cn
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References
Civan MM, Marano CW, Matschinsky FW et al (1994) Prolonged incubation with elevated glucose inhibits the regulatory response to shrinkage of cultured human retinal pigment epithelial cells. J Membr Biol 139(1):1–13
Hong J, Yuan Z, Shuai J et al (2007) High glucose induce production of iNOS by human retinal pigment epithelium cells through activation of the p38 signal pathway. Acta Universitatis Medicinalis Nanjing (Natural Science) 27(9):970–973
Izumi N, Nagaoka T, Mori F et al (2006) Relation between plasma nitric oxide levels and diabetic retinopathy. Jpn J Ophthalmol 50(5):465–468
Kanaze FI, Gabrieli C, Kokkalou E et al (2003) Simultaneous reversed-phase high-performance liquid chromatographic method for the determination of diosmin, hesperidin and naringin in different citrus fruit juices and pharmaceutical formulations. J Pharm Biomed Anal 33(2):243–249
Leal EC, Manivannan A, Hosoya K et al (2007) Inducible nitric oxide synthase isoform is a key mediator of leukostasis and blood-retinal barrier breakdown in diabetic retinopathy. Invest Ophthalmol Vis Sci 48(11):5257–5265
Luo X, Li S (2008) Progress in Biologic Activity Effects of Hesperidin. J Gannan Med Univ 28(2):301–304
Madsen-Bouterse SA, Kowluru RA (2008) Oxidative stress and diabetic retinopathy: pathophysiological mechanisms and treatment perspectives. Rev Endocr Metab Disord 9(4):315–327
Rao YK, Fang SH, Tzeng YM (2008) Antiinflammatory activities of flavonoids and a triterpene caffeate isolated from Bauhinia variegata. Phytother Res 22(7):957–962
Roufail E, Soulis T, Boel E et al (1998) Depletion of nitric oxide synthase-containing neurons in the diabetic retina: reversal by aminoguanidine. Diabetologia 41(12):1419–1425
Sakata K, Hirose Y, Qiao Z et al (2003) Inhibition of inducible isoforms of cyclooxygenase and nitric oxide synthase by flavonoid hesperidin in mouse macrophage cell line. Cancer Lett 199(2):139–145
Shashi W, Tapas CN (1999) Nitric oxide synthase immunoreactivity in the developing and adult human retina. J Biosci 24(4):483–490
Toda N, Nakanishi-Toda M (2007) Nitric oxide: ocular blood flow, glaucoma, and diabetic retinopathy. Prog Retin Eye Res 26(3):205–238
Tommasini S, Calabrò ML, Stancanelli R et al (2005) The inclusion complexes of hesperetin and its 7-rhamnoglucoside with (2-hydroxypropyl)-beta-cyclodextrin. J Pharm Biomed Anal 39(3–4):572–580
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Xiaoting, L., Xiangyun, Z., Shumei, L., Minghua, D., Liang, X. (2010). Effect of Hesperidin on Expression of Inducible Nitric Oxide Synthase in Cultured Rabbit Retinal Pigment Epithelial Cells. In: Anderson, R., Hollyfield, J., LaVail, M. (eds) Retinal Degenerative Diseases. Advances in Experimental Medicine and Biology, vol 664. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-1399-9_22
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DOI: https://doi.org/10.1007/978-1-4419-1399-9_22
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