Abstract
Introduction: An essential role for metalloproteases (MMPs) has been described in blood vessel neoformation and the removal of cell debris. MMPs also play a key role in degenerative processes and in tumors. The participation of these enzymes in light-induced phototoxic processes is supported by both experimental and clinical data. Given that patients with age-related macular degeneration often show deposits, or drusen, these deposits could be the consequence of deficient MMP production by the pigment epithelium.
Objective: To gain insight into the regulation of metalloproteases in the pathogenia of retinal degeneration induced by light.
Materials and Methods: We examined the eyes of experimental rabbits exposed for 2 years to circadian cycles of white light, blue light and white light lacking short wavelengths. For the trial the animals had been implanted with a transparent intraocular lens (IOL) and a yellow AcrySof® IOL, one in each eye. After sacrificing the animals, the retinal layer was dissected from the eye and processed for gene expression analyses in which we examined the behavior of MMP-2, MMP-3 and MMP-9.
Results: MMP-2 expression was unaffected by the light received and type of IOL. However, animals exposed to white light devoid of short wavelengths or those fitted with a yellow IOL showed 2.9- and 3.6-fold increases in MMP-3 expression, respectively compared to controls. MMP-9 expression levels were also 3.1 times higher following exposure to blue light and 4.6 times higher following exposure to white light lacking short wavelengths or 4.2 times higher in eyes implanted with a yellow IOL.
Conclusion: Exposure to long periods of light irrespective of its characteristics leads to the increased expression of some MMPs. This alteration could indicate damage to the extracellular matrix and have detrimental effects on the retina.
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References
Chen L, Wu W, Dentychev T et al (2004) Light damage induced changes in mouse retinal gene expression. Exp Eye Res 79:239–247
Curran T, Franza BR Jr. (1988) Fos and Jun: the AP-1 connection. Cell 55:395–397
Elliot S, Catanuto P, Stetler-Stevenson W et al (2006) Retinal pigment epithelium protection from oxidant-mediated loss of MMP-2 activation requires both MMP-14 and TIMP-2. Invest Ophthalmol Vis Sci 47:1696–1702
Flaxel C, Bradle J, Acott T et al (2007) Retinal pigment epithelium produces matrix metalloproteinases after laser treatment. Retina 27:629–634
Fujieda H, Sasaki H (2008) Expression of brain-derived neurotrophic factor in cholinergic and dopaminergic amacrine cells in the rat retina and the effects of constant light rearing. Exp Eye Res 86:335–343
Gauthier R, Joly S, Pernet V et al (2005) Brain-derived neurotrophic factor gene delivery to muller glia preserves structure and function of light-damaged photoreceptors. Invest Ophthalmol Vis Sci 46:3383–3392
Grimm C, Wenzel C, Hafezi F et al (2000) Gene expression in the mouse retina: effect of damaging light. Mol Vis 6:252–260
Llamosas MM, Cernuda-Cernuda R, Huerta JJ et al (1997) Neurotrophin receptors expression in the developing mouse retina: an immunohistochemical study. Anat Embryol (Berl) 195:337–344
LĂłpez-OtĂn C, Overall CM (2002) Protease degradomics: a new challenge for proteomics. Nat Rev Mol Cell Biol 3:509–519
Margrain TH, Boulton M, Marshall J et al (2004) Do blue light filters confer protection against age-related macular degeneration?. Prog Retin Eye Res 23:523–531
Meyers SM (2004) A model of spectral filtering to reduce photochemical damage in age-related macular degeneration. Trans Am Ophtalmol Soc 102:83–95
Papp AM, Nyilas R, Szepesi Z et al (2007) Visible light induces matrix metalloproteinase-9 expression in rat eye. J Neurochem 103:2224–2233
Plantner JJ (1992) The presence of neutral metalloproteolytic activity and metalloproteinase inhibitors in the interphotoreceptor matrix. Curr Eye Res 11:91–101
Plantner JJ, Drew TA (1994) Polarized distribution of metalloproteinases in the bovine interphotoreceptor matrix. Exp Eye Res 59:577–585
Plantner JJ, Le ML, Kean EL (1991) Enzymatic deglycosylation of bovine rhodopsin. Exp Eye Res 53:269–274
Plantner JJ, Jiang C, Smine A (1998) Increase in interphotoreceptor matrix gelatinase A (MMP-2) associated with age-related macular degeneration. Exp Eye Res 67:637–645
Plantner JJ, Smine A, Quinn TA (1998) Matrix metalloproteinases and metalloproteinase inhibitors in human interphotoreceptor matrix and vitreous. Curr Eye Res 17:132–140
Seiler MJ, Thomas BB, Chen Z, Arai S, Chadalavada S, Mahoney MJ, Sadda SR, Aramant RB (2008) BDNF-treated retinal progenitor sheets transplanted to degenerate rats – Improved restoration of visual function. Exp Eye Res 86:92–104
Thanos C, Emerich D (2005) Delivery of neurotrophic factors and therapeutic proteins for retinal diseases. Expert Opin Biol Ther 5:1443–1452
Wenzel A, Reme CE, Williams TP et al (2001) The Rpe65 Leu450Met variation increases retinal resistance against light-induced degeneration by slowing rhodopsin regeneration. J Neurosci 21:53–58
Wu J, Seregard S, Algvere PV (2006) Photochemical damage of the retina. Surv Ophthalmol 51:461–481
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Sanchez-Ramos, C., Vega, J.A., del Valle, M., Fernandez-Balbuena, A., Bonnin-Arias, C., Benitez-del Castillo, J.M. (2010). Role of Metalloproteases in Retinal Degeneration Induced by Violet and Blue Light. In: Anderson, R., Hollyfield, J., LaVail, M. (eds) Retinal Degenerative Diseases. Advances in Experimental Medicine and Biology, vol 664. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-1399-9_19
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DOI: https://doi.org/10.1007/978-1-4419-1399-9_19
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