Abstract
Electron microscopic immunocytochemistry takes advantage of the increased resolution of the electron microscope to localize label to specific cellular organelles. Optical microscopes limit resolution to the wavelength of light, about 0.2 μm or 200 nm. The electron microscope has theoretical resolution of less than 0.2 nm. Practically for biological samples, resolution is limited to about 2 nm because of the epoxy resin embedding. Polymers in the epoxy resin scatter the electron beam and limit the resolution of cellular structures.
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© 2010 Springer-Verlag New York
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Burry, R.W. (2010). Electron Microscopic Immunocytochemistry. In: Immunocytochemistry. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-1304-3_15
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DOI: https://doi.org/10.1007/978-1-4419-1304-3_15
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Print ISBN: 978-1-4419-1303-6
Online ISBN: 978-1-4419-1304-3
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