Multiple Antibodies from the Same Species

Chapter

Abstract

Using 1° antibodies made in the different species is the easiest way to localize multiple proteins ( Chapter 11). However, it is not possible to get all 1° antibodies needed from a different species. This is especially true because there are so many mouse monoclonal antibodies available. Eventually, an experiment will need two mouse 1° antibodies or two rabbit 1° antibodies.

Keywords

Immunohistochemistry Antibody labeling Fluorescence microscopy Fluorescent immunocytohemistry Fluorescent immunohistochemistry Indirect immunocytochemistry Immunostaining 

Copyright information

© Springer-Verlag New York 2010

Authors and Affiliations

  1. 1.College of Medicine & Public Health Ohio State UniversityColumbusUSA

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