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Fluorescence Imaging of Calcium Loading and Mitochondrial Depolarization in Cancer Cells Exposed to Heat Stress

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Reviews in Fluorescence 2008

Part of the book series: Reviews in Fluorescence 2008 ((RFLU,volume 2008))

Abstract

One main issue of thermotherapy is the stress response of mitochondria to heat. Thermotherapies function by inducing lethal heat inside target tissues. Actually spatial and temporal instabilities of temperature distributions inside target volumes require optimized treatment protocols and reliable temperature-control methods during thermotherapies. Since solid tumors present predominant targets to thermotherapy, on the one hand, the hyperthermic stress-induced effects on mitochondrial transmembrane potentials in breast cancer cells (MX1) were analyzed. On the other hand, the intracellular Ca2+ fluctuations in different cell types responding to heat stress were investigated.

Heat sensitivities and stress reactions might be extremely different among different tissue species and tissue dignities; therefore, it is very important to investigate tissue-specific stress responses systematically. Even though this chapter contributes little information, only, to the enlightenment of systemic cellular heat stress mechanisms, it may support the fortification of basic knowledge about systemic stress responses. Using cytoplasmic and intramitochondrial fluorescent Ca2+ probes it was possible to compare hyperthermia-induced changes in the Ca2+ distribution between the cytoplasm and the mitochondria of normal and tumor cells and to examine the relationship between mitochondrial Ca2+ concentration and changes in the viability of these cell types upon hyperthermic treatment. We compared Ca2+ concentrations in cytoplasm and mitochondria in cancerous CX1 and MX1 cells with normal CHO cells after transfer from room temperature (25°C) to 37°C or 43°C. Sudden increase in the incubation temperature (from room temperature to 37°C) induced very different cytoplasmic and mitochondrial Ca2+ fluctuation patterns in normal CHO and CX1 and MX1 tumor cells. Estimating the CX1, MX1, and CHO cell viabilities upon hyperthermic treatment, we found that thermosensitivities increase in the sequence CX1<CHO<MX1. CHO cells were not less sensitive to hyperthermia than were MX1 tumor cells, but results show that the lowest amount of calcium is in the CHO cells, whereas the highest mitochondrial Ca2+ is in the most thermosensitive MX1 cells. The preliminary results are consistent with the conclusion that the sensitivities of cancer cells to hyperthermic treatments depend on the initial mitochondrial Ca2+ concentrations. However, more experiments are needed to confirm these suggestions. Further on, the data presented here might support an optimization of the treatment protocols applied during thermotherapy, particularly LITT and hyperthermia.

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Authors and Affiliations

  1. Jürgen Beuthan, Urszula Zabaryło: Charité – Universitätsmedizin Berlin, CC6 AG Medizinische Physik / Optische Diagnostik, Fabeckstr. 60-62, 14195, Berlin, Germany

    Olaf Minet

  2. LMTB GmbH, Fabeckstr. 60-62, 14195, Berlin, Germany

    Cathrin Dressler

  3. Charité – Universitätsmedizin Berlin, CC6 AG Medizinische Physik/Optische Diagnostik, Fabeckstr. 60-62, 14195, Berlin, Germany

    Jürgen Beuthan & Urszula Zabaryło

  4. Vytautas Magnus University, Vileikos 8, LT-44404, Kaunas, Lithuania

    Rasa Zukiene & Vida Midaziene

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  1. Olaf Minet
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  1. Institute of Fluorescence, University of Maryland, 701 E. Pratt St., Suites 3017-21, Baltimore, MD, 21202, USA

    Chris D. Geddes

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Minet, O., Dressler, C., Beuthan, J., Zabaryło, U., Zukiene, R., Midaziene, V. (2010). Fluorescence Imaging of Calcium Loading and Mitochondrial Depolarization in Cancer Cells Exposed to Heat Stress. In: Geddes, C.D. (eds) Reviews in Fluorescence 2008. Reviews in Fluorescence 2008, vol 2008. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-1260-2_4

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