The Role of Glyoxalase System in Renal Hypoxia

  • Reiko Inagi
  • Takanori Kumagai
  • Toshiro Fujita
  • Masaomi Nangaku
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 662)


Methylglyoxal (MG), a highly reactive α-oxoaldehyde generated by oxidation of carbohydrate and glycolysis, binds to proteins and forms advanced glycation end products (AGE). MG and MG adducts have been implicated in oxidative stress-related diseases, therefore, MG detoxifying system such as the glyoxalase system (glyoxalase I) also contributes to progression of these diseases. Recent papers have emphasized the pathophysiological effects of MG and the glyoxalase system in acute hypoxic injury, which is associated with acute oxidative stress. We investigated the kinetics of MG level and glyoxalase I activity in renal acute hypoxic injury induced by ischemia-reperfusion (I/R). I/R induced tubulointerstitial injury and the histological changes were associated with a significant decrease in renal glyoxalase I activity and an increase in MG level in the damaged tubular cells. Of note, rats over expressing human glyoxalase I showed amelioration of I/R-induced histological and functional damages and it was associated with a decrease in MG level in the lesion resulting in reduction of oxidative stress and tubular cell apoptosis. In conclusion, glyoxalase I has renoprotective effects in renal hypoxia such as I/R injury via a reduction in cytotoxic MG level in tubular cells.


Hyperglycemic Condition Advanced Glycation Endproducts Mitochondrial Reactive Oxygen Species Production Hypoxic Injury Glyoxalase System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by Grants-in-Aid for Scientific Research from Japan Society for the Promotion of Science (19590939 to RI and 19390228 to MN).


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Reiko Inagi
    • 1
  • Takanori Kumagai
    • 2
  • Toshiro Fujita
    • 2
  • Masaomi Nangaku
    • 2
  1. 1.Division of Nephrology and EndocrinologyUniversity of Tokyo School of MedicineTokyoJapan
  2. 2.Division of Nephrology and EndocrinologyUniversity of Tokyo School of MedicineTokyoJapan

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