Abstract
In order to obtain evidence that Mb releases O2 during muscle contraction, we have set up a buffer-perfused hindlimb rat model and applied NIRS to detect the dynamics of tissue deoxygenation during contraction. The NIRS signal was monitored on hindlimb muscle during twitch contractions at 1 Hz, evoked via electrostimulator at different submaximal levels. The hindlimb perfusion was carried out by perfusion of Krebs Bicarbonate buffer. The NIRS still detected a strong signal even under Hb-free contractions. The deoxygenation signal (Δ[deoxy]) was progressively increased at onset of the contraction and reached the plateau under both blood- and buffer-perfused conditions. However, the amplitude of Δ[deoxy] during steady state continued to significantly increase as tension increased. The tension-matched comparison of the Δ[deoxy] level under buffer-perfused and blood perfused conditions indicate that Mb can contribute approximately 50% to the NIRS signal. These results clarify the Mb contribution to the NIRS signal and show a falling intracellular PO2 as workload increases.
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Acknowledgments
This research was supported by a Grant-in-Aid for Scientific Research from the Japanese Ministry of Education, Science, Sports and Culture (grant #: 18700527, KM), and by the Uehara Memorial Foundation.
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Masuda, K., Takakura, H., Furuichi, Y., Iwase, S., Jue, T. (2010). NIRS Measurement of O2 Dynamics in Contracting Blood and Buffer Perfused Hindlimb Muscle. In: Takahashi, E., Bruley, D. (eds) Oxygen Transport to Tissue XXXI. Advances in Experimental Medicine and Biology, vol 662. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-1241-1_46
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DOI: https://doi.org/10.1007/978-1-4419-1241-1_46
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