Abstract
In order to evaluate the effect of cerebral ischemia on the flavoprotein fluorescence (FPF), we compared the changes in the FPF and somatosensory evoked potential (SEP) during transient cerebral ischemia in the rat. We measured the FPF and SEP simultaneously via a cranial window made over the right sensorimotor cortex during the left median nerve stimulation in F344 rats. We compared change in FPF and SEP during cerebral ischemia for 60 min. The rCBF were rapidly recovered after reperfusion. However, the recovery rates of the FPF were significantly faster than those of the SEP after reperfusion. These findings indicate that activity-dependent changes of the FPF do not necessarily correlate with the electrical activity after transient cerebral ischemia.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Araque A, Parpura V, Sanzgiri RP et al. (1998) Glutamate-dependent astrocyte modulation of synaptic transmission between cultured hippocampal neurons. Eur J Neurosci 10:2129–2142.
Buchheim K, Wessel O, Siegmund H et al. (2005) Processes and components participating in the generation of intrinsic optical signal changes in vitro. Eur J Neurosci 22:125–132.
Chance B, Legallais V (1963) A spectrofluorometer for recording of intracellular oxidation-reduction states. IEEE Trans Biomed Eng 10:40–47.
Husson TR, Mallik AK, Zhang JX et al. (2007) Functional imaging of primary visual cortex using flavoprotein autofluorescence. J Neurosci 27:8665–8675
Koizumi S, Saito Y, Nakazawa K et al. (2002) Spatial and temporal aspects of Ca2+ signaling mediated by P2Y receptors in cultured rat hippocampal astrocytes. Life Sci 72:431–442.
Reinert KC, Dunbar RL, Gao W et al. (2004) Flavoprotein autofluorescence imaging of neuronal activation in the cerebellar cortex in vivo. J Neurophysiol 92:199–211.
Shibuki K, Hishida R, Murakami H et al. (2003) Dynamic imaging of somatosensory cortical activity in the rat visualized by flavoprotein autofluorescence. J Physiol 549:919–927.
Siesjö BK (1988) Mechanisms of ischemic brain damage. Crit Care Med 16:954–963.
Takahashi K, Hishida R, Kubota Y et al. (2006) Transcranial fluorescence imaging of auditory cortical plasticity regulated by acoustic environments in mice. Eur J Neurosci 23:1365–1376.
Tohmi M, Kitaura H, Komagata S et al (2006) Enduring critical period plasticity visualized by transcranial flavoprotein imaging in mouse primary visual cortex. J Neurosci 26:11775–11785.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Springer Science+Business Media, LLC
About this paper
Cite this paper
Igarashi, T. et al. (2010). Effect of Transient Forebrain Ischemia on Flavoprotein Autofluorescence and the Somatosensory Evoked Potential in the Rat. In: Takahashi, E., Bruley, D. (eds) Oxygen Transport to Tissue XXXI. Advances in Experimental Medicine and Biology, vol 662. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-1241-1_13
Download citation
DOI: https://doi.org/10.1007/978-1-4419-1241-1_13
Published:
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4419-1239-8
Online ISBN: 978-1-4419-1241-1
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)