Abstract
This chapter covers fixation, staining and coverslipping of frozen section slides. A number of fixatives and fixative cocktails in use are described and illustrated as well as artifacts resulting from prolonged drying. Several commonly used staining procedures are described with suggestions on maximizing the quality and information gathered for interpretation. Adhesion of tissue to glass slides and causes of poor adhesion is discussed. Problems arising in the coverslipping process are discussed and method for coverslipping finished slides is offered.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Fishman A, Lew S, Altaras M, Beyth Y, Bernheim J (1998) A 30s PAS stain for frozen section analysis of surgical margins of vulvectomy in Paget’s disease. Eur J Gynaecol Oncol 19(5):482–483
Histonet list server web link http://www.histonet.org/
Kiernan JA (1999) Strategies for preventing detachment of sections from glass slides. Micros Today 99–96:22–24
Klatt, Edward MD (2008) The Internet Pathology Laboratory for Medical Education. Mercer University School of Medicine, Savannah http://library.med.utah.edu/WebPath/webpath.html#MENU
Suchetha Soans MD, Lorenzo M, Galindo MD, Fernando U, Garcia MD (1999) Mucin stain on Frozen sections a rapid 3-minute method. Arch Pathol Lab Med 123(5):378–380
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Springer Science+Business Media, LLC
About this chapter
Cite this chapter
Peters, S.R., Delia, C.S. (2010). Fixation, Staining and Coverslipping of Frozen Section Slides. In: Peters, S. (eds) A Practical Guide to Frozen Section Technique. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-1234-3_6
Download citation
DOI: https://doi.org/10.1007/978-1-4419-1234-3_6
Published:
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4419-1233-6
Online ISBN: 978-1-4419-1234-3
eBook Packages: MedicineMedicine (R0)