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Role of the Growth-Associated Protein GAP-43 in NCAM-Mediated Neurite Outgrowth

  • Irina KorshunovaEmail author
  • Mark Mosevitsky
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 663)

Abstract

The neural cell adhesion molecule (NCAM) can regulate actin cytoskeletal dynamics through associations with growth-associated protein-43 (GAP-43). By binding to the fibroblast growth factor receptor (FGFR), NCAM activates intracellular pathways to trigger calcium release, lipid diacylglycerol (DAG) formation, and protein kinase C (PKC) activation to specifically phosphorylate GAP-43 on serine 41. Phosphorylated GAP-43 plays a key role in neurite outgrowth, presumably by promoting actin polymerization. Of all NCAM isoforms, only NCAM-180 takes part in this GAP-43-dependent neurite outgrowth. GAP-43 and NCAM-180 are found in the same plasma membrane domains (rafts), and these two proteins form a functional complex with spectrin that may control cytoskeleton dynamics to induce neurite outgrowth. In the absence of GAP-43, the signaling pathway that depends on NCAM-140 and nonreceptor tyrosine kinase (Fyn) is activated.

Keywords

NCAM-180 NCAM-140 GAP-43 Signal transduction Lipid rafts Neurite outgrowth 

Notes

Acknowledgments

The authors gratefully acknowledge the financial support of the Danish Medical and Natural Science Research Councils, the Lundbeck Foundation, Danish Cancer Society, European Union 6FP, PROMEMORIA, LSHM-CT-2005-512012, and RFBR grants 06-04-08336 and 06-04-48943.

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© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Protein Laboratory, Department of Neuroscience and PharmacologyUniversity of CopenhagenCopenhagenDenmark
  2. 2.Division of Molecular and Radiation Biophysics, Petersburg Nuclear Physics InstituteRussian Academy of SciencesGatchinaRussia

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