Advertisement

Kinetics of Chemo/Biosensors

Chapter

Abstract

An insight into biosensor kinetics has been presented in this chapter with a focus on analyte–receptor interactions on biosensor surfaces. The vast applications of sensors are rapidly increasing, especially in the areas of medicine, food and drug administration and detection of toxic substances in various fields, and industrial applications. The Surface Plasmon Resonance (SPR) Biosensor is a dynamic equipment for measuring signals in analyte–receptor systems and is the main medium through which all the data in this chapter are obtained. A fractal analysis (single and dual) is presented here in an attempt to model the kinetic data obtained from literature to obtain important kinetic parameters such as the binding and dissociation rate constants, affinity, and fractal dimension values.

Examples are cited for the binding of liquid petroleum gas (LPG) to zinc oxide films prepared by the spray pyrolysis method onto a glass substrate (Sens and Actuator B 120: 551–559, 2007) and the binding of different NH3 concentrations in air to a sol-gel derived thin film (Sens and Actuator B 110:299–303, 2005). Since the major focus of this chapter is the kinetics, only scant information, if any, is presented on thermodynamics in these system interactions. However, fractal analysis provides a unique perspective of these molecular interactions and could be applied in other systems.

Keywords

Biosensors Kinetics Binding Dissociation Fractals 

Abbreviations

SPR

Surface plasmon resonance

LPG

Liquid petroleum gas

Sens

Sensors

BST

Barium strontium titanate

λexo

λexonuclease

TYPNK

T4 polynucleotide kinase

FAM-SP-2p

Oligonucleotide sequence (5-3′)

ATP

Adenosine triphosphate

DNA

Deoxyribonucleic acid

ATR

Attenuated total reflection

BIA

Biomolecular interaction analysis

SPs

Surface plasmons

UV

Ultraviolet

IR

Infrared

Ag

Antigen

Ab

Antibody

References

  1. Berg HC, Purcell EM (1977) Physics of chemoreception. Biophys J 20(2):193–219CrossRefGoogle Scholar
  2. Chai CC, Peng J, Yan BP (1995) Preparation and gas-sensing properties of Fe2O3 thin films. J Electron Mater 24:82–86CrossRefGoogle Scholar
  3. Chaplin MF (2010) Structuring and behaviour of water in nanochannels and confined spaces. In: (ed) Adsorption and phase behavior in nanochannels and nanotubes, Springer, in pressGoogle Scholar
  4. Dewey TG (1997) Fractals in molecular biophysics. Oxford University, New York, NYMATHGoogle Scholar
  5. Dewey TG, Bann JG (1992) Protein dynamics and 1/f noise. Biophys J 63:594–598CrossRefGoogle Scholar
  6. Havlin S (1989) The fractal approach to heterogeneous chemistry: surfaces, colloids, polymers. Wiley, New York, pp 251–269Google Scholar
  7. Homola J, Gauglitz G, Yee SS (1999) Surface plasmon resonance sensors: review. Sens Actuator B 54:3–15CrossRefGoogle Scholar
  8. Ismail B, Abaab MA, Rezig B (2001) Structural and electrical properties of ZnO films prepared by screen printing technique. Thin Solid Films 383:92–94CrossRefGoogle Scholar
  9. June LS, Campbell CT, Chinowsky TM, Mar MN, Yee SS (1998) Quantitative interpretation of the response of surface plasmon resonance sensors to adsorbed films. Langmuir 12:5636–5648Google Scholar
  10. Kang K, Redner S (1985) Fluctuation-dominated kinetics in diffusion-controlled reactions. Phys Rev A 32:435CrossRefGoogle Scholar
  11. Knemeyer JP, Marine N, Sauer M (2000) Probes for detection of specific DNA sequences at the single-molecule level. Anal Chem 72:3717–3724CrossRefGoogle Scholar
  12. Kooyman RPH, Kolkman H, Gent JV, Greve J (1988) Surface plasmon resonance immunosensors: sensitivity considerations. Anal Chem Acta 213:35–45CrossRefGoogle Scholar
  13. Kopelman R (1988) Fractal reaction kinetics. Science 241:1620–1626CrossRefGoogle Scholar
  14. Korotcenkov G, Brinzari V, Schwank J, DiBattista M, Visiliev A (2001) Pecularities of SnO2 thin film deposition by spray pyrolysis for gas sensor applications. Sens and Actuator B 77:244–252CrossRefGoogle Scholar
  15. Liebovitch LS, Sullivan JM (1987) Fractal analysis of a voltage-dependent potassium channel from cultured mouse hippocampal neurons. Biophysics J 52(6):979–988CrossRefGoogle Scholar
  16. Liedberg B, Nylander C, Lundström I (1983) Surface plasmon resonance for gas detection and biosensing. Sens and Actuators 4:299–304CrossRefGoogle Scholar
  17. Liu XQ, Tao SW, Shen YS (1997) Preparation and characterization of nanocrystalline α-Fe2O3 by a sol-gel process. Sens and Actuator B 40:161–165CrossRefGoogle Scholar
  18. Martin SJ, Granstaff VE, Frye GC (1991) Effect of surface roughness on the response of thickness-shear mode resonators in liquids. Anal Chem 65:2910–2922CrossRefGoogle Scholar
  19. Masel IR (1996) Principles of adsorption and reaction on solid surfaces. John Wiley & Sons, New York, NYGoogle Scholar
  20. Melikhova EM, Kurochkin IN, Zaitsev SV, Varfolomeev SD (1988) Analysis of ligand-receptor binding by the difference method. Anal Biochem 175(2):507–15CrossRefGoogle Scholar
  21. Owen V (1997) Real-time optical immunosensors - a commercial reality. Biosens Bioelectron 12:1–2CrossRefMathSciNetGoogle Scholar
  22. Patil PS (1999) Versatility of chemical spray pyrolysis technique. Mater Chem Phys 59:185–198CrossRefMathSciNetGoogle Scholar
  23. Peterlinz KA, Georgiadis RM, Herne TM, Tarlov MJ (1997) Observation of hybridization and dehybridization of thiol-tethered DNA using two-color surface plasmon resonance spectroscopy. J Am Chem Soc 119:3401–3402CrossRefGoogle Scholar
  24. Ramakrishnan A, Sadana A (2001) A fractal analysis for cellular analyte–receptor binding kinetics; biosensor applications. Automedica, 1–28Google Scholar
  25. Rao GST, Rao DT (1999) Gas sensitivity of ZnO based thick film sensor to NH3 at room temperature. Sens and Actuator B 55:166–169CrossRefGoogle Scholar
  26. Roy SC, Sharma GL, Bhatnagar MC, Samanta SB (2005) Novel ammonia sensing phenomena in sol-gel derived Ba0.5ISr0.5TiO3 thin films. Sens and Actuator B 110:299–303CrossRefGoogle Scholar
  27. Sadana A (2001) A kinetic study of analyte–receptor binding and dissociation, and dissociation alone for biosensor application;a fractal analysis. Anal Biochem 291(1):34–47CrossRefGoogle Scholar
  28. Shinde VR, Gujar TP, Lokhande CD (2007) LPG sensing properties of ZnO films prepared by spray pyrolysis method. Effect of molarity of precursor solution. Sens and Actuator B 120:551–559CrossRefGoogle Scholar
  29. Song C, Zhao M (2009) Real-time monitoring of the activity and kinetics of T4 polynucleotide kinase by a singly-labeled DNA hairpin smart probe coupled with exonuclease cleavage. Anal Chem 81:1383–1388CrossRefGoogle Scholar
  30. Wojciechowski BW (1975) Chemical kinetics for chemical engineers. Sterling Swift publishing company, New York, NYGoogle Scholar
  31. Zhu W, Tan OK, Yan Q, Oh JT (1993) Microstructure and hydrogen gas sensitivity of amorphous BST thin film sensors. Sens and Actuator B 85:205–211Google Scholar
  32. Zwanzig R, Szabo A (1991) Time dependent rate of diffusion-influenced ligand binding to receptors on cell surfaces. Biophys J 60:671–678CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Chemical Engineering DepartmentUniversity of MississippiUniversityUSA

Personalised recommendations