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Synchrony of the Rat Medial Prefrontal Cortex Network During Isoflurane Anaesthesia

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Book cover The Basal Ganglia IX

Part of the book series: Advances in Behavioral Biology ((ABBI,volume 58))

Abstract

In the anaesthetized rat, the membrane potential of the prefrontal cortex (PFC) fluctuates between two states of polarization: the up- and down-states. The PFC network strongly governs activity in its target areas such as various basal ganglia structures. The influence of anaesthesia on network dynamics in the prelimbic cortex will therefore have direct consequences for the activity profile of the output structures. In order to investigate the effects of isoflurane anaesthesia on network activity of the PFC, rats were anaesthetized with isoflurane of which the concentration was varied between 1.25 and 2.25%. Local EEG and single-unit activity were recorded simultaneously.

At baseline levels (1.75%), isoflurane anaesthesia induced up-state transitions in the prelimbic cortex visible in the local EEG signal. The up-state deflections in the EEG were determined as reflections of clustered firing of individual prelimbic neurons. 2.25% isoflurane strongly reduced the up-state frequency whereas 1.25% isoflurane allowed the network to shift to a continuous activity mode. These results show that neuronal activity in the medal PFC and therefore its target structures is highly dependent on the level of isoflurane anaesthesia. Considering the similarity between the synaptic targets of isoflurane by which surgical anaesthesia is maintained and the mechanisms involved in initiation, maintenance and cessation of up-states, the up- and down-state fluctuations are a direct result of anaesthesia.

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Authors and Affiliations

  1. Department of Anatomy and Neurosciences, Cognitive Neurophysiology-CNCR, VU University Medical Center, 7057, 1007, MB, Amsterdam, The Netherlands

    Mathijs Stegeman, Marieke de Boer, Marcel van der Roest & Antonius B. Mulder

Authors
  1. Mathijs Stegeman
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  2. Marieke de Boer
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  3. Marcel van der Roest
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  4. Antonius B. Mulder
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Corresponding author

Correspondence to Antonius B. Mulder .

Editor information

Editors and Affiliations

  1. Dept. Anatomy & Neurosciences, Vrije Universiteit Amsterdam, Amsterdam, 1007 MB, Netherlands

    Hendrik Jan Groenewegen

  2. Medical Center, Vrije Universiteit, Van der Boechorststraat 7, Amsterdam, 1081 BT, Netherlands

    Pieter Voorn

  3. Department of Neurology, Vrije Universiteit Medical Center, De Boelelaan 1117, Amsterdam, 1081 HV, Netherlands

    Henk W. Berendse

  4. Medical Center, Vrije Universiteit, Van der Boechorststraat 7, Amsterdam, 1081 BT, Netherlands

    Antonius B. Mulder

  5. Medical Centre, Radboud University Nijmegen, Geert Grooteplein 21a, Nijmegen, 6525 EZ, Netherlands

    Alexander R. Cools

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Stegeman, M., de Boer, M., van der Roest, M., Mulder, A.B. (2009). Synchrony of the Rat Medial Prefrontal Cortex Network During Isoflurane Anaesthesia. In: Groenewegen, H., Voorn, P., Berendse, H., Mulder, A., Cools, A. (eds) The Basal Ganglia IX. Advances in Behavioral Biology, vol 58. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-0340-2_11

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  • DOI: https://doi.org/10.1007/978-1-4419-0340-2_11

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