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V(D)J Recombination pp 116–132Cite as

Molecular Genetics at the T-Cell Receptor β Locus: Insights into the Regulation of V(D)J Recombination

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Part of the book series: Advances in Experimental Medicine and Biology ((volume 650))

Abstract

The V(D)J recombination machinery assembles antigen receptor genes from germline V,D and J segments during lymphocyte development. In αβT cells, this leads to the production of the T-cell receptor (TCR) α and β chains. Notably, V(D)J recombination at the Tcrb locus is tightly controlled at various levels, including cell-type and stage specificities, intralocus ordering and allelic exclusion. Although many of these controls are partly mediated at the level of genomic accessibility to the V(D)J recombinase, recent studies have uncovered novel mechanisms that are also likely to contribute to the developmental regulation of Tcrb gene rearrangement events. In this chapter, we summarize our current knowledge and highlight unanswered questions regarding the regulation of V(D)J recombination at the Tcrb locus, placing emphasis on mouse transgenesis and gene-targeting approaches.

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  1. Centre d’Immunologie de Marseille-Luminy, Université d’Aix Marseille CNRS, UMR 6102 Inserm, U 631, Marseille, France

    Marie Bonnet, Pierre Ferrier & Salvatore Spicuglia

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  1. Marie Bonnet
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  2. Pierre Ferrier
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  3. Salvatore Spicuglia
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Correspondence to Salvatore Spicuglia .

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  1. Centre d’Immunologie de Marseille-Luminy, Université d’Aix Marseille, Marseille, France

    Pierre Ferrier MD, PhD

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Bonnet, M., Ferrier, P., Spicuglia, S. (2009). Molecular Genetics at the T-Cell Receptor β Locus: Insights into the Regulation of V(D)J Recombination. In: Ferrier, P. (eds) V(D)J Recombination. Advances in Experimental Medicine and Biology, vol 650. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-0296-2_10

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