Abstract
In this chapter, we bring together up-to-date information concerning plant cell biotechnology and its applications. Because plants contain many valuable secondary metabolites that are useful as drug sources (pharmaceuticals), natural fungicides and insecticides (agrochemicals), natural food flavorings and coloring agents (nutrition), and natural fragrances and oils (cosmetics), the production of these phytochemicals through plant cell factories is an alternative and concurrent approach to chemical synthesis. It also provides an alternative to extraction of these metabolites from overcollected plant species. While plant cell cultures provide a viable system for the production of these compounds in laboratories, its application in industry is still limited due to frequently low yields of the metabolites of interest or the feasibility of the bioprocess. A number of factors may contribute to the efficiency of plant cells to produce desired compounds. Genetic stability of cell lines, optimization of culture condition, tissue-diverse vs. tissue-specific site-specific localization and biosynthesis of metabolites, organelle targeting, and inducible vs. constitutive expression of specific genes should all be taken into consideration when designing a plant-based production system. The major aims for engineering secondary metabolism in plant cells are to increase the content of desired secondary compounds, to lower the levels of undesirable compounds, and to introduce novel compound production into specific plants. Recent achievements have also been made in altering various metabolic pathways by use of specific genes encoding biosynthetic enzymes or genes that encode regulatory proteins. Gene and metabolic engineering approaches are now being used to successfully achieve highest possible levels of value-added natural products in plant cell cultures. Applications through functional genomics and systems biology make plant cell biotechnology much more straightforward and more attractive than through previous, more traditional approaches.
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References
Apse, M.P., Aharon, G.S., Snedden, W.A., Blumwald, E. 1999. Salt tolerance conferred by overexpression of a vacuolar Na super(+)/H super(+) antiport in Arabidopsis. Science 285: 1256–1258.
Bailey, J.E., Sburlati, A., Hatzimanikatis, V., Lee, K.H., Renner, W.A., Tsai, P.S. 1996. Inverse metabolic engineering: a strategy for directed genetic engineering of useful phenotypes. Biotechnology & Bioengineering 52: 109–121.
Bailey, J.E., Sburlati, A., Hatzimanikatis, V., Lee, K., Renner, W.A., Tsai, P.S. 2002. Inverse metabolic engineering: a strategy for directed genetic engineering of useful phenotypes. Biotechnology & Bioengineering 79(5): 568–579.
Basset, G.J.C., Quinlivan, E.P., Gregory III, J.F., Hanson, A.D. 2005. Folate synthesis and metabolism in plants and prospects for biofortification. Crop Science 45: 449–453.
Carrari, F., Urbanczyk-Wochniak, E., Willmitzer, L., Fernie, A.R. 2003. Engineering central metabolism in crop species: learning the system. Metabolic Engineering 5: 191–200.
Chartrain, M., Salmon, P.M., Robinson, D.K., Buckland, B.C. 2000. Metabolic engineering and directed evolution for the production of pharmaceuticals. Current Opinion in Biotechnology 11: 209–214.
Cseke, L., Kirakosyan, A., Kaufman, P., Warber, S., Duke, J., Brielmann, H. 2006. Natural Products from Plants, 2nd ed. CRC Press/Taylor & Francis Group, Boca Raton, FL, p. 616.
DellaPenna, D. 2001. Plant metabolic engineering. Plant Physiology 125: 160–163.
Delmer, D.P., Haigler, C.H. 2002. The regulation of metabolic flux to cellulose, a major sink for carbon in plants. Metabolic Engineering 4: 22–28.
Dixon, R.A. 2005. Engineering of plant natural product pathways. Current Opinion in Plant Biology 8: 329–336.
Dos Santos, R.J., Schripsema, J., Verpoorte, R. 1994. Ajmalicine metabolism in Catharanthus roseus cell cultures. Phytochemistry 35: 677–681.
Gambonnet, B., Jabrin, S., Ravanel, S., Karan, M., Douce, R., Rebeille, F. 2001. Folate distribution during higher plant development. Journal of the Science of Food and Agriculture 81: 835–841.
Giuliano, G., Tavazza, R., Diretto, G., Beyer, P., Taylor, M.A. 2008. Metabolic engineering of carotenoid biosynthesis in plants. Trends in Biotechnology 26: 139–145.
Kasuga, M., Liu, Q., Miura, S., Yamaguchi-Shinozaki, K., Shinozak, K. 1999. Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducible transcription factor. Nature Biotechnology 17: 287–291.
Kim, B., Gibson, D.M., Shuler, M.L. 2005. Relationship of viability and apoptosis to taxol production in taxus sp. suspension cultures elicited with methyl jasmonate. Biotechnology Progress 21: 700–707.
Kleeb, A.C., Edalat, H.M., Gamper, M., Haugstetter, J., Giger, L., Neuenschwander, M., Kast, P., Hilvert, D. 2007. Metabolic engineering of a genetic selection system with tunable stringency. PNAS 104: 13907–13912.
Kunze, R., Frommer, W.B., Flügge, U.I. 2002. Metabolic engineering of plants: the role of membrane transport. Metabolic Engineering 4(1): 57–66.
Maliga, P., Graham, I. 2004. Molecular farming and metabolic engineering promise a new generation of high-tech crops. Current Opinion in Plant Biology 7: 149–151.
Morgan, J.A., Shanks, J.V. 2002. Quantification of metabolic flux in plant secondary metabolism by a biogenetic organizational approach. Metabolic Engineering 4: 257–262.
Ohmiya, A., Kishimoto, S., Aida, R., Yoshioka, S., Sumitomo, K. 2006. Carotenoid cleavage dioxygenase (CmCCD4a) contributes to white color formation in chrysanthemum petals. Plant Physiology 142: 1193–1201.
Plackett, R.L., Burman, J.P. 1946. The design of optimum multifactorial experiments. Biometrica 33: 305–325.
Roberts, S.C., Shuler, M.L. 1997. Large-scale plant cell culture. Current Opinion in Biotechnology 8: 154–159.
Romer, S., Lubeck, J., Kauder, F., Steiger, S., Adomat, C., Sandmann, G. 2002. Genetic engineering of a zeaxanthin-rich potato by antisense inactivation and co-suppression of carotenoid epoxidation. Metabolic Engineering 4: 263–272.
Rontein, D., Basset, G., Hanson, A.D. 2002. Metabolic engineering of osmoprotectant accumulation in plants. Metabolic Engineering 4: 49–56.
Sakamoto, A., Murata, N. 1998. Metabolic engineering of rice leading to biosynthesis of glycinebetaine and tolerance to salt and coldis one of the best example in this field. Plant Molecular Biology 38: 1011–1019.
Sauer, U., Schlattner, U. 2004. Inverse metabolic engineering with phosphagen kinase systems improves the cellular energy state. Metabolic Engineering 6: 220–228.
Verpoorte, R. 1996. Plant cell biotechnological research in the Netherlands. In DiCosmo, F., Misawa, M. (eds.) Plant Cell Culture Secondary Metabolism. CRC Press, Boca Raton, New York, London, Tokyo, p. 660.
Verpoorte, R., Alfermann, A.W. 2000. Metabolic Engineering of Plant Secondary Metabolism. Kluwer Academic Publishers Group, The Netherlands, p. 296.
Verpoorte, R., Memelink, J. 2002. Engineering secondary metabolite production in plants. Current Opinion in Biotechnology 13: 181–187.
Verpoorte, R., van der Heijden, R., Hoge, J.H.C., ten Hoopen, H.J.G. 1994. Plant cell biotechnology for the production of secondary metabolites. Pure and Applied Chemistry 66: 2307–2310.
Verpoorte, R., van der Heijden, R., Memelink, J. 2000. Engineering the plant cell factory for secondary metabolite production. Transgenic Research 9: 323–343.
Verpoorte, R., van der Heijden, R., ten Hoopen, H.J.G., Memelink, J. 1999. Metabolic engineering of plant secondary metabolite pathways for the production of fine chemicals. Biotechnology Letters 21: 467–479.
Vogel, H.C., Tadaro, C.L. 1997. Fermentation and Biochemical Engineering Handbook – Principles, Process Design, and Equipment, 2nd ed. William Andrew Publishing, Noyes, p. 801.
Welsch, R., Maass, D., Voegel, T., DellaPenna, D., Beyer, P. 2007. Transcription factor RAP2.2 and its interacting partner SINAT2: Stable elements in the carotenogenesis of arabidopsis leaves. Plant Physiology 145: 1073–1085.
Yu, O., Jez, J.M. 2008. Nature’s assembly line: biosynthesis of simple phenylpropanoids and polyketides. The Plant Journal 54(4): 750–762.
Yu, O., Shi, J., Hession, A.O., Maxwell, C.A., McGonigle, B., Odell, J.T. 2003. Metabolic engineering to increase isoflavone biosynthesis in soybean seed. Phytochemistry 63(7): 753–763.
Zenk, M.H., El-Shagi, H., Arens, H., Stockigt, J., Weiler, E.W., Deus, B. 1977. Formation of indole alkaloids serpentine and ajmalicine in cell suspension cultures of Catharanthus roseus. In Barz, W., Reinhard, E., Zenk, M.H. (eds.) In Plant Tissue Culture and Its Biotechnological Application. Springer Verlag, Berlin, Germany, pp. 27–43.
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Kirakosyan, A., Cseke, L.J., Kaufman, P.B. (2009). The Use of Plant Cell Biotechnology for the Production of Phytochemicals. In: Recent Advances in Plant Biotechnology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-0194-1_2
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DOI: https://doi.org/10.1007/978-1-4419-0194-1_2
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