Production of Bioactive Secondary Metabolites

  • Poonam Singh nee’ Nigam


This chapter includes information based on published literature on utilization of agro industrial residues for the production of bioactive compounds. Various approaches using microbial fermentation technology have been explored for the production of bioactive compounds which as secondary metabolites could be produced by selected microorganisms. Certain factors have been found to affect the productivity of these compounds, hence the yield of secondary metabolites may be manipulated by controlling these factors in fermentation system.


Secondary metabolite Stationary phase Idiophase Biologically-active compounds Antibiotics Mycotoxins Ergot-alkaloids 


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  1. Balakrishnan K, Pandey A (1996) Production of biologically active secondary metabolites in solid state fermentation. J Sci Ind Res 55: 365–372Google Scholar
  2. Chang SB, Abdel-Kadar M.M, Wick EL, Wogan GN (1963) Aflatoxin B2: chemical identity and biological activity. Science 142: 1191–1192PubMedCrossRefGoogle Scholar
  3. Collemare J, Billard A, Bohnert HU, Lebrun MH (2008) Biosynthesis of secondary metabolites in the rice blast fungus Magnaporthe grisea: the role of hybrid PKS-NRPS in pathogenicity. Mycol Res Feb 112(Pt 2): 207–215CrossRefGoogle Scholar
  4. Datar R (1986) Economics of primary separation steps in relation to fermentation and genetic engineering. Process Biochem 21: 19–26Google Scholar
  5. Demain AL, Hunt AN, Malik V, Kobbe B, Hawkins H, Matsuo K, Wogan GN (1977) Improved procedure for production of cytochalasin E and tremorgenic toxins by Aspergillus clavatus. Appl Environ. Microbiol 31: 138–140Google Scholar
  6. Ghose AC, Manmade A, Townsend JM, Bosquet A, Howes JF, Demain AL (1978) Production of cyclochlorotine and a new metabolite, simatoxin by Penicillium islandicum. Appl Environ Microbiol 35: 1074–1078Google Scholar
  7. Greenhalgh R, Neish GA, Miller D (1983) Deoxynivalenol, acetyl deoxynivalenol, and zearalenone formation by Canadian isolates of Fusarium graminarium on solid substrates. Appl Environ Microbiol 46: 625–629PubMedGoogle Scholar
  8. Hesseltine CW (1977) Solid state fermentation-Part I. Process Biochem 12(6): 24–27Google Scholar
  9. Jermini MFG, Demain AL (1989) Solid state fermentation for cephalosporin production by Streptomyces clauvligerus and Cephalosporin acremonium. Experienta 45: 1061–1065CrossRefGoogle Scholar
  10. Khaldi N, Collemare J, Lebrun MH, Wolfe KH (2008, Jan 24) Evidence for horizontal transfer of a secondary metabolite gene cluster between fungi. Genome Biol 9(1): R18PubMedCrossRefGoogle Scholar
  11. Kobbe B, Cushman M, Wogan GN, Demain AL (1977) Production and antibacterial activity of malformin C, a toxic metabolite of Aspergillus niger. Appl Environ Microbiol 33: 996–997PubMedGoogle Scholar
  12. Kumar PKR, Lonsane BK (1987a) Gibberellic acid by SSF: consistent and improved yields. Biotechnol Bioeng 30: 267–271PubMedCrossRefGoogle Scholar
  13. Kumar PKR, Lonsane BK (1987b) Extraction of gibberellic acid from dry mouldy bran produced under solid-state fermentation. Process Biochem 22: 139–143Google Scholar
  14. Kumar PKR, Lonsane BK (1987c) Potential of fed-batch culture in solid-state fermentation for the production of gibberellic acid. Biotechnol Lett 9: 179–182CrossRefGoogle Scholar
  15. Kumar PKR, Lonsane BK (1988) Batch and fed-batch solid-state fermentations: kinetics of cell growth, hydrolytic enzymes production, and gibberellic acid production. Process Biochem 23(2): 43–47Google Scholar
  16. Lin CF, Lizuka H (1982) Production of pigment by a mutant of Monascus kaoliang sp. nov. Appl Environ Microbiol 43: 671–676PubMedGoogle Scholar
  17. Lindenfelser LA, Ciegler A (1975) Solid state fermenter for ochratoxin A production. Appl Microbiol 29: 323–327PubMedGoogle Scholar
  18. Luk KC, Kobbe B, Townsend JM (1977) Production of cyclopiazonic acid by Aspergillus flavus. Appl Environ Microbiol 33: 211–212PubMedGoogle Scholar
  19. Nigam P, Singh D (2000) Secondary Metabolites. In Encyclopedea of Food Microbiology (RK Robinson et al. eds.) Academic Publisher, LondonGoogle Scholar
  20. Nigam P, Singh D (1999) Characteristics and techniques of fermentation systems in Biotechnology: Food Fermentation, Vol. II, (VK Joshi, A Pandey eds) Educational Publishers, N Delhi, pp. 427–466Google Scholar
  21. Nigam P, Singh D (1996a) Processing of agricultural wastes in solid state fermentation for microbial protein production. J Sci Ind Res 55: 373–380Google Scholar
  22. Nigam P, Singh D (1996b) Processing of agricultural wastes in solid state fermentation for cellulolytic enzyme production. J Sci Ind Res 55: 457–467Google Scholar
  23. Nigam P, Singh D (1994) Solid-state (substrate) fermentation systems and their applications in biotechnology. J Basic Microbiol 34: 405–423CrossRefGoogle Scholar
  24. Pandey A, Soccol CR, Nigam P, Soccol VT, Vandenberghe LPS, Mohan R (2000a) Biotechnological potential of agro-industrial residues: II cassava bagasse. Bioresource Technol 74(1): 81–87CrossRefGoogle Scholar
  25. Pandey A, Soccol CR, Nigam P, Soccol VT (2000b) Biotechnological potential of agro-industrial residues: I sugarcane bagasse. Bioresource Technol 74(1): 69–80CrossRefGoogle Scholar
  26. Pandey A, Soccol CR, Rodriguez-Leon JA and Nigam P (2001) Production of organic acids by solid state fermentation. In Solid state fermentation in Biotechnology-Fundamentals and Applications, Asitech Publishers N. Delhi, pp. 132–158Google Scholar
  27. Patron NJ, Walker RF, Cozijnsen Aj et al. (2007) Origin and distribution of epipolythiodioxopiperazine (ETP) gene clusters in filamentous ascomycetes. BMS Evol Biol Sep 26(7): 174–179CrossRefGoogle Scholar
  28. Robinson T, Nigam P, Singh D (2004) Secondary metabolites. In Handbook of Fungal Biotechnology, (DK Arora et al. eds.) Marcel Dekker Inc, NY pp. 267–274 ISBN 08247-4018-1Google Scholar
  29. Robinson T, Singh D, Nigam P (2001) Solid-state fermentation: A promising microbial technology for secondary metabolite production. Appl Microbio Biotechnol 55: 284–289CrossRefGoogle Scholar
  30. Shotwell OL, Hesseltine CW, Stubblefield RD, Sorenson WG (1966) Appl Microbiol 14: 425–428PubMedGoogle Scholar
  31. Silman RW, Conway HF, Anderson RA, Bagley EB (1979) Production of aflatoxin in corn by a large-scale solid-substrate fermentation process. Biotechno Bioeng 21: 1799–1808CrossRefGoogle Scholar

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© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Poonam Singh nee’ Nigam
    • 1
  1. 1.Faculty of Life and Health Sciences, School of Biomedical SciencesUniversity of UlsterColeraineNorthern Ireland, UK

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