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Evaluation of Metarhizium anisopliae (Deuteromycota: Hyphomycetes) for control of broad mite Polyphagotarsonemus latus (Acari: Tarsonemidae) in mulberry

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Diseases of Mites and Ticks

Abstract

A study on 12 entomopathogenic fungi for controlling broad mite (Polyphagotarsonemus latus (Banks)) in mulberry found that Metarhizium anisopliae CKM-048 was the most virulent strain in controlling both larvae and adult broad mites at the concentration of 2 × 108 conidia/ml. There was no ovicidal effect when tested with broad mite eggs. Median lethal concentrations (LC50) of M. anisopliae in killing larvae and adults were 8.7 × 106 and 1.3 × 107conidia/ml, respectively. Median lethal times (LT50) of larvae and adults were 2.4 and 3.8 days, respectively, at the concentration of 2 × 108 conidia/ml. The fungus was found to produce protease and chitinase. Scanning electron microscope (SEM) studies were done to monitor the infection steps of the fungus on broad mites. A greenhouse test on mulberry trees revealed that M. anisopliae could reduce the broad mite population within 4 days after treatment. However, after 7 days, its efficacy was decreased significantly.

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References

  • Abbott WS (1925) A method of computing the effectiveness of an insecticide. J Econ Entomol 18:265–267

    CAS  Google Scholar 

  • Bonants PJM, Fitter PFL, Thijs H, den Belder E, Waalwijk C, Henfling JWDM (1995) A basic serine protease from Paecilomyces lilacianus with biological activity against Meloidogyne hapla eggs. Microbiology 141:775–784

    Article  PubMed  CAS  Google Scholar 

  • Chatdumrong V (1996) Microbial selection for chitin digesting enzyme production. MS thesis, Kasetsart University, Bangkok (in Thai)

    Google Scholar 

  • Chongcharoen R, Vatanyoopaisan S (2005) Study of lipase production in bacteria isolated from natural and industrial sources. Kasetsart J (Nat Sci) 39:161–166

    Google Scholar 

  • Cuthbertson AGS, Walters KFA (2002) Pathenogenecity of the entomopathogenic fungus, Lecanicillium muscarium, against the sweet potato whitefly Bemisia tabaci under laboratory and greenhouse conditions. Mycopathologia 160:315–319

    Article  Google Scholar 

  • Dackman C, Chet I, Hertz BN (1989) Fungal parasitism of the cyst nematode Heterodera schachtii: infection and enzyme activity. FEMS Microbiol Ecol 62:201–208

    Article  CAS  Google Scholar 

  • Dawson RMC, Elliott DC, Elliott WH, Jones KM (1969) Data for biochemical research. Oxford University Press, London, 592 pp

    Google Scholar 

  • Elad Y, Chet I, Henis Y (1982) Dedradation of plant pathogenic fungi by Trichoderma harzianum. Can J Microbiol 28:719–725

    Article  CAS  Google Scholar 

  • Finney DJ (1971) Probit analysis, 3rd edn. Cambridge University Press, Cambridge, 333 pp

    Google Scholar 

  • Gerson U (1992) Biology and control of the broad mite, Polyphagotarsonemus latus (Banks) (Acari: Tarsonemidae). Exp Appl Acarol 13:163–178

    Article  Google Scholar 

  • Ibrahim YB, Low W (1998) Life cycle of the broad mite Polyphagotarsonemus latus (Bank) (Acari): Prostigmata; Tarsonemidae and its innate proliferating capacity on chili. Malaysian Appl Biol 27:51–56

    Google Scholar 

  • Jeppson LR, Keifer HH, Baker EW (1975) Mite injurious to economic plants. University of California Press, Berkeley

    Google Scholar 

  • Kemsawasd J (1976) Leaf curl disease of chili caused by the broad mite Polyphagotarsonemus latus (Banks) and its control. MS thesis, Kasetsart University, Bangkok (in Thai)

    Google Scholar 

  • Khan A, Williums K, Molloy MP, Nevalainen H (2003) Purification and characterization of a serine protease and chitinase activity on 2D gels. Protein Expr Purif 32:210–220

    Article  PubMed  CAS  Google Scholar 

  • Luangsa-Ard JJ, Hywel-Jones NL, Mamoch L, Samson RA (2005) On the relationships of Paecilomyces sect. Isarioidea species. Mycol Res 109(5):581–589

    Article  PubMed  CAS  Google Scholar 

  • Lovrien RE, Gusek T, Hart B (1985) Cellulase and protease specific activities of commercially available cellulase preparations. J Appl Biochem 7:258–272

    CAS  Google Scholar 

  • Lowry OH, Rosebourgh NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275

    PubMed  CAS  Google Scholar 

  • Meikle WG, Mercadier G, Rosengaus RB, Kirk AA, Derouane F, Ouimby PC (2005) Evaluation of an entomopathogenic fungus, Paecilomyces fumosoroseus (Wize) Brown and Smith (Deuteromycota: Hyphomycetes) obtained from Formosan subterranean termites (Isop.: Rhinotermitidae). J Appl Entomol 129(6):315–322

    Article  Google Scholar 

  • Nugroho I, Ibrahim Y (2004) Laboratory bioassay of some entomophathogenic fungi against broad mite. Inter J Agric Biol 6(2):223–225

    Google Scholar 

  • Palevsky E, Soroker V, Weintraub P, Mansour F, Abo-Moch F, Gerson U (2001) How species-specific is the phoretic relationship between the broad mite, Polyphagotarsonemus latus (Acari:Tarsonemidae), and its insect host? Exp Appl Acarol 25(3):217–224

    Article  PubMed  CAS  Google Scholar 

  • Pena JE, Campbell CW (2005) Broad mite. EDIS. http://edis.ifas.ufl.edu/CHO2O, 13 September 2005.

  • Wagner BL, Lewis LC (2000) Colonization of Corn, Zea mays, by the Entomopathogenic Fungus Beauveria bassiana. Appl Environ Microbiol 66(8):3468–3473

    Article  PubMed  CAS  Google Scholar 

  • Yang OH, Chen CX (1982) A study on Polyphagotarsonemus latus Banks. Kunchong Zhishi 19:24–26

    Google Scholar 

  • Zare R, Gams W (2001) A revision of Verticillium section Prostrata IV. The genera Lecanicillium and Simlicillium gen. nov. Nova Hedwigia 73:1–50

    Google Scholar 

Download references

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Correspondence to Monchan Maketon .

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Maketon, M., Orosz-Coghlan, P., Sinprasert, J. (2008). Evaluation of Metarhizium anisopliae (Deuteromycota: Hyphomycetes) for control of broad mite Polyphagotarsonemus latus (Acari: Tarsonemidae) in mulberry. In: Bruin, J., van der Geest, L.P.S. (eds) Diseases of Mites and Ticks. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-9695-2_13

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