Abstract
Caenorhabditis elegans was the first multicellular organism to have its genome sequenced and has proved useful in the investigations of innate immunity, the generic science that underpins the biology of host-pathogen interactions. This chapter explores the sequencing of plant-parasitic nematodes and microbial genomes and shows how this knowledge can help in understanding the biology of the interaction between Meloidogyne spp. and the bacterial nematode parasite Pasteuria penetrans. Three examples examine how genomic information can help in developing new approaches to the problems associated with using Pasteuria as a biological control agent: initially one focuses on the transportome and how genomics might help to understand the fastidious nature of Pasteuria growth in the nematode; secondly, comparative genomics is used to explore the phosphorylation pathway important in initiating sporulation; and, thirdly, comparative genomics is exploited to understand endospore attachment to the nematode cuticle where, in comparisons with other animal parasitic Bacillus spp., collagen-like fibres have been implicated. Finally the chapter suggests that genomics paves the way for the development of designer control agents but such an approach would not be without its critics.
Keywords
- Biological Control Agent
- Major Facilitator Superfamily
- Operonic Structure
- Nematode Cuticle
- Solute Binding Protein
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Acknowledgments
KGD and THM would like to thank The Biotechnological and Biological Sciences Research Council of the United Kingdom. This work was also supported in part by the North Carolina Biotechnology Centre and the North Carolina Agricultural Research Service.
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Schaff, J.E., Mauchline, T.H., Opperman, C.H., Davies, K.G. (2011). Exploiting Genomics to Understand the Interactions Between Root-Knot Nematodes and Pasteuria penetrans . In: Davies, K., Spiegel, Y. (eds) Biological Control of Plant-Parasitic Nematodes:. Progress in Biological Control, vol 11. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-9648-8_4
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