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Overexpression of Conserved Kinase UNC-51 Inhibits the Transferrin's Endocytosis into the Mammalian Cells

  • Huaize Tian
  • Sanetaka Shirahata
Conference paper
Part of the Animal Cell Technology: Basic & Applied Aspects book series (ANICELLTECH, volume 15)

Abstract

UNC-51 overexpression can inhibit the transferrin endocytosis in the transfected COS-7 cells. We think that this inhibition of transferrin endocytosis should be caused by UNC-51-dependent microtubule-disruption (being submitted to Science Signaling).

Keywords

kinase UNC-51 tubulin actin dynamics endocytosis transferrin 

Notes

Acknowledgments

We are very thankful to Drs. K. Hayashi and T. Tahira, T. Tani, K. Nakayama, A. Yamanaka, K. Mizuno, K. Tawada, Y. Emoto, H. Arata, K. Katayama, Y. Fujiki, Y. Fukumaki, M. Kimura, S. Osaki, I. Ito and Y. Kato for kindly providing pCAGGS vector, COS cells, HeLa cells, HEK293T cells, pUcD2SRαMCS, Beckman Optima centrifuge, French press, the computer system, kind discussion, advice, help and encouragement. We express sincere gratitude to all members of both laboratories for their help and encouragement.

References

  1. 1.
    Riezman H, Woodman PG, Meer G, Marsh M (1997). Molecular mechanisms of endocytosis. Cell 91, 731–738.CrossRefPubMedGoogle Scholar
  2. 2.
    Marsh M and McMahon HT (1999). The structural era of endocytosis. Science 285, 215–220.CrossRefPubMedGoogle Scholar
  3. 3.
    Shintani T, Klionsky DJ (2004). Autophagy in health and disease: a double-edged sword. Science. 306, 990–995.CrossRefPubMedGoogle Scholar
  4. 4.
    Jin M and Snider MD (1993). Role of microtubules in transferring receptor transport from the cell surface to endosomes and the Golgi complex. J. Biol. Chem. 268, 18390–18397.PubMedGoogle Scholar
  5. 5.
    Thatte HS, Bridges KR, Golan DE (1994). Microtubule inhibitors differentially affect translational movement, cell surface expression, and endocytosis of transferring receptors in K562 cells. J. Cell Physiol. 160, 345–357.CrossRefPubMedGoogle Scholar
  6. 6.
    Elkjaer ML, Birn H, Agre P, Christensen EI, Nielsen S (1995). Effects of microtubule disruption on endocytosis, membrane recycling and polarized distribution of Aquaporin-1 and gp330 in proximal tubule cells. Eur. J. Cell Biol. 67, 57–72.PubMedGoogle Scholar
  7. 7.
    Subtil A, Dautry-Varsat A (1997). Microtubule depolymerization inhibits clathrin coated-pit internalization in non-adherent cell lines while interleukin 2 endocytosis is not affected. J. Cell Sci. 110, 2441–2447.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Huaize Tian
    • 1
    • 2
    • 3
  • Sanetaka Shirahata
    • 2
  1. 1.Department of Biology, Faculty of ScienceKyushu UniversityFukuokaJapan
  2. 2.Laboratory of Cellular Regulation Technology, Graduate School of Systems Life SciencesKyushu UniversityFukuokaJapan
  3. 3.Neuronal Neo-Cytoskeletonology, International International Health and Life Science Center Co., Ltd.FukuokaJapan

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