Overexpression of Conserved Kinase UNC-51 Inhibits the Transferrin's Endocytosis into the Mammalian Cells

  • Huaize Tian
  • Sanetaka Shirahata
Conference paper
Part of the Animal Cell Technology: Basic & Applied Aspects book series (ANICELLTECH, volume 15)


UNC-51 overexpression can inhibit the transferrin endocytosis in the transfected COS-7 cells. We think that this inhibition of transferrin endocytosis should be caused by UNC-51-dependent microtubule-disruption (being submitted to Science Signaling).


kinase UNC-51 tubulin actin dynamics endocytosis transferrin 



We are very thankful to Drs. K. Hayashi and T. Tahira, T. Tani, K. Nakayama, A. Yamanaka, K. Mizuno, K. Tawada, Y. Emoto, H. Arata, K. Katayama, Y. Fujiki, Y. Fukumaki, M. Kimura, S. Osaki, I. Ito and Y. Kato for kindly providing pCAGGS vector, COS cells, HeLa cells, HEK293T cells, pUcD2SRαMCS, Beckman Optima centrifuge, French press, the computer system, kind discussion, advice, help and encouragement. We express sincere gratitude to all members of both laboratories for their help and encouragement.


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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Huaize Tian
    • 1
    • 2
    • 3
  • Sanetaka Shirahata
    • 2
  1. 1.Department of Biology, Faculty of ScienceKyushu UniversityFukuokaJapan
  2. 2.Laboratory of Cellular Regulation Technology, Graduate School of Systems Life SciencesKyushu UniversityFukuokaJapan
  3. 3.Neuronal Neo-Cytoskeletonology, International International Health and Life Science Center Co., Ltd.FukuokaJapan

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