CCAAT/Enhancer-Binding Protein Beta Controls Differentiation-Specific Expression of Chromatin Remodeling Factor BRM
The chromatin remodeling complex, SWI/SNF exclusively contains BRG1 or BRM as an ATPase subunit. So far, we have confirmed that the expression of BRM was very low in neural precursor cells and fetal liver cells, and induced to a high level in terminally differentiated neurons and astrocytes, and adult liver cells. The expression of BRM was very low in undifferentiated P19 cells and was induced to a high level during differentiation. In contrast, BRG1 was constantly expressed throughout differentiation. In this study, we analyzed developmental regulation of the brm gene. Luciferase reporter assays showed an increase in the transcriptional activity of the BRM promoter during differentiation of P19 cells. The promoter region of the brm gene contains two putative binding sites for CCAAT/enhancer-binding protein beta (C/EBPβ). Luciferase reporter assays showed that the binding of transcription factors C/EBPβ might stimulate the transcriptional activity of the BRM promoter.
KeywordsSWI/SNF neural cell differentiation C/EBPβ
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