Abstract
T cells have been used for cancer immunotherapy because they play a central role in the cellular immunity. One approach to cure cancers is that tumor-specific T cells are activated in vitro and subsequently injected into patients. However, desired therapeutic effect has not been attained due to difficulty to maintain activated T cells for a prolonged period after injection. To solve this problem, we thought to use an interleukin (IL)-2-dependent growth signal in T cells for maintenance of activated T cells. If non-toxic molecules can substitute for the function of IL-2, genetically modified T cells can be expanded selectively without IL-2 and no side effect would occur. In this study, we replaced IL-2 binding domains of IL-2 receptor (IL-2R) β and γ chains by antibody variable regions recognizing a cognate antigen. Because these chimeric IL-2Rs could replace an IL-2-mediated signal by an antigen-mediated one, only the T cells with these chimeric receptors would grow in the presence of the cognate antigen but without IL-2.
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Sogo, T., Kawahara, M., Ueda, H., Nagamune, T. (2008). Selective Expansion of Genetically Modified T Cells Using a Chimeric IL-2 Receptor for Cancer Therapy. In: Shirahata, S., Ikura, K., Nagao, M., Ichikawa, A., Teruya, K. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 15. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-9646-4_2
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DOI: https://doi.org/10.1007/978-1-4020-9646-4_2
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