A Serum Substitute for Fed-Batch Culture of Hybridoma Cells

  • Keisuke Shibuya
  • Ryoichi Haga
  • Masaru Namba
Conference paper
Part of the Animal Cell Technology: Basic & Applied Aspects book series (ANICELLTECH, volume 15)

Abstract

A serum substitute consisting of 12 components has been developed to produce fed-batch cultures of hybridoma cells in serum-free medium. We did a fed-batch culture of hybridoma cells using this substitute and confirmed that the cells could be successfully cultivated this way. The specific production rates of lactate and ammonia, which are harmful byproducts from cells, were significantly reduced compared with a conventional serum-containing batch culture. This reduction led to a higher cell concentration and a longer production lifetime. As a result, the final concentration of monoclonal antibody was 400 mg/l, or five times greater than that in the conventional serum-containing batch culture. The developed substitute will enable fed-batch cultivation in a serum-free condition.

Keywords

Serum-free culture fed-batch culture hybridoma cells 

References

  1. 1.
    Khoudi H, Laberge S, Ferullo JM, Bazin R, Darveau A, Castonguary Y, Allard G, Lemieux R and Vezina LP, Production of a diagnostic monoclonal antibody in perennial Alfalfa plants. Biotechnol Bioeng 64, 135–143 (1999).CrossRefPubMedGoogle Scholar
  2. 2.
    Liddell E and Weeks I, Anitibody Technology. Bios Scientific, UK, pp. 65–130 (1995).Google Scholar
  3. 3.
    Spier RE, Animal cell biotechnology in the 1990s, from models to morals, in Animal Cell Biotechnology, Eds. Spier RE and Griffiths JB. Cambridge, Academic Press, UK, pp. 1–43 (1994).Google Scholar
  4. 4.
    Bibila TA and Robinson DK, In pursuit of the optimal fed-batch process for monoclonal ­antibody production. Biotechnol Prog 11, 1–13 (1995)CrossRefPubMedGoogle Scholar
  5. 5.
    Distefano DJ, Mark GE and Robinson DK, Feeding of nutrients delays apoptotic death in fed-batch cultures of recombinant NS0 myeloma cells. Biotechnol Lett 18, 1067–1072 (1996).CrossRefGoogle Scholar
  6. 6.
    Birch JR and Racher AJ, Antibody production. Adv Drug Deliv Rev 58(5–6), 671–85 (2006).CrossRefPubMedGoogle Scholar
  7. 7.
    Xie L and Wang DI, Applications of improved stoichiometric model in medium design and fed-batch cultivation of animal cells in bioreactor. Cytotechnology 15(1-3), 17–29 (1994)CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Keisuke Shibuya
    • 1
  • Ryoichi Haga
    • 1
  • Masaru Namba
    • 1
  1. 1.Hitachi, Ltd. Power & Industrial Systems R&D LaboratoryIbarakiJapan

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