A Serum Substitute for Fed-Batch Culture of Hybridoma Cells

  • Keisuke Shibuya
  • Ryoichi Haga
  • Masaru Namba
Conference paper
Part of the Animal Cell Technology: Basic & Applied Aspects book series (ANICELLTECH, volume 15)


A serum substitute consisting of 12 components has been developed to produce fed-batch cultures of hybridoma cells in serum-free medium. We did a fed-batch culture of hybridoma cells using this substitute and confirmed that the cells could be successfully cultivated this way. The specific production rates of lactate and ammonia, which are harmful byproducts from cells, were significantly reduced compared with a conventional serum-containing batch culture. This reduction led to a higher cell concentration and a longer production lifetime. As a result, the final concentration of monoclonal antibody was 400 mg/l, or five times greater than that in the conventional serum-containing batch culture. The developed substitute will enable fed-batch cultivation in a serum-free condition.


Serum-free culture fed-batch culture hybridoma cells 


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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Keisuke Shibuya
    • 1
  • Ryoichi Haga
    • 1
  • Masaru Namba
    • 1
  1. 1.Hitachi, Ltd. Power & Industrial Systems R&D LaboratoryIbarakiJapan

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