A Serum Substitute for Fed-Batch Culture of Hybridoma Cells
A serum substitute consisting of 12 components has been developed to produce fed-batch cultures of hybridoma cells in serum-free medium. We did a fed-batch culture of hybridoma cells using this substitute and confirmed that the cells could be successfully cultivated this way. The specific production rates of lactate and ammonia, which are harmful byproducts from cells, were significantly reduced compared with a conventional serum-containing batch culture. This reduction led to a higher cell concentration and a longer production lifetime. As a result, the final concentration of monoclonal antibody was 400 mg/l, or five times greater than that in the conventional serum-containing batch culture. The developed substitute will enable fed-batch cultivation in a serum-free condition.
KeywordsSerum-free culture fed-batch culture hybridoma cells
- 2.Liddell E and Weeks I, Anitibody Technology. Bios Scientific, UK, pp. 65–130 (1995).Google Scholar
- 3.Spier RE, Animal cell biotechnology in the 1990s, from models to morals, in Animal Cell Biotechnology, Eds. Spier RE and Griffiths JB. Cambridge, Academic Press, UK, pp. 1–43 (1994).Google Scholar