Skip to main content

Effect of alkali treatment time and extraction time on agar from Gracilaria vermiculophylla

  • Chapter
Nineteenth International Seaweed Symposium

Abstract

The effects of alkali treatment time and extraction time of native agar and alkali treated agar obtained from Gracilaria vermiculophylla were studied. The response characteristics were mainly agar yield and gel strength. Alkali-treatment was carried out at 0.5, 1.0, 1.5, 2.0, 2.5, and 3.0 h. Agar yield and gel strength decreased with the increase in the time of the alkali-treatment. The highest yield (15.3%) and highest gel strength (1,064 g cm−2) were obtained at 0.5 h, and therefore this time was used for the next experiment. The extraction of both native and alkali treated agars was carried out at 1.5, 2.0, 2.5, and 3.0 h. The best extraction time for alkali treated agar was 1.5 h, and for native agar 2.5 h. The alkali treated agar obtained with the different alkali-treatment and extraction times showed higher melting (92.4–99.7°C) and gelling (35.7–39.6°C) temperatures. Native agar was lower in melting (60.2–64.1°C) and gelling (20.4–23.4°C) temperatures. The 3,6-anhydrogalactose content decreased with increasing alkali-treatment time, with the opposite effect during the extraction of native and alkali treated agars.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Araki C (1966) Some recent studies on the polysaccharides of agarophytes. Proc Int Seaweed Symp 5:3–19

    Google Scholar 

  • Armisen R (1995) World-wide use and importance of Gracilaria. J Appl Phycol 7:231–243

    Article  Google Scholar 

  • Armisen R, Galatas F (1987) Production, properties and uses of agar. In: McHugh DJ (ed) Production and utilization of products from commercial seaweeds. Fish Tech Paper 288. FAO

    Google Scholar 

  • Armisen R, Galatas F (2000) Agar. In: Phillips GO, Williams PA (eds) Handbook of hydrocolloids. CRC, Boca Raton, Fla.

    Google Scholar 

  • Chirapart A, Katou Y, Ukeda H et al (1995) Physical and chemical properties of agar from a new member of Gracilaria, G. lemaneiformis (Gracilariales, Rhodophyta) in Japan. Fish Sci 61:450–454

    CAS  Google Scholar 

  • Duckworth M, Yaphe W (1971) The structure of agar. Part 1. Fractionation of a complex mixture of polysaccharides. Carbohydr Res 16:359–366

    Google Scholar 

  • Freile-Pelegrín Y, Robledo D (1997) Influence of alkali treatment on agar from Gracilaria cornea from Yucatan, Mexico. J Appl Phycol 9:533–539

    Google Scholar 

  • Freile-Pelegrín Y, Murano E (2004) Agars from three species of Gracilaria (Rhodophyta) from Yucatán Peninsula. Bioresour Technol 96:295–302

    Article  CAS  Google Scholar 

  • Hurtado-Ponce AQ, Umezaki I (1988) Physical properties of agar gel from Gracilaria (Rhodophyta) of the Philippines. Bot Mar 31:171–174

    Article  Google Scholar 

  • Mollet JC, Rahaoui A, Lemmoine Y (1998) Yield, chemical composition and gel strength of agarocolloids of Gracilaria gracilis, Gracilariopsis longissima and the newly reported Gracilaria c.f. vermiculophylla from Roscoff (Brittany, France). J Appl Phycol 10:59–66

    Article  CAS  Google Scholar 

  • Okazaki A (1971) Seaweeds and their uses in Japan. Tokai University Press

    Google Scholar 

  • Ramalingam JR, Kaliaperumal N, Kalimuthu S (2002) Agar production from Gracilaria with improved qualities. Seaweed Res Utiln 24(1):25–34

    Google Scholar 

  • Rebello J, Ohno M, Ukeda H, Sawamura M (1997) Agar quality of commercial agarophytes from different geographical origins: 1. Physical and rheological properties. J Appl Phycol 8:517–521

    Article  Google Scholar 

  • Santelices B (1988) Synopsis of biological data on the seaweed genera Gelidium and Pterocladia (Rhodophyta). FAO Fish Synop 145

    Google Scholar 

  • Vergara-Rodarte MA (2006) Tendencias poblacionales de Gracilaria vermiculophylla (Ohmi) Papen. (Gracilariales, Rhodophyta) en la Laguna San Ignacio, B.C.S., durante un ciclo anual. Bachelor dissertation, Universidad Autónoma de Baja California Sur

    Google Scholar 

  • Villanueva RD, Pagba CV, Montaño NE (1997) Optimized agar extraction from Gracilaria eucheumoides Harvey. Bot Mar 40:369–372

    CAS  Google Scholar 

  • Yaphe W (1984) Properties of Gracilaria agars. Hydrobiologia 116/117:171–186

    Article  Google Scholar 

  • Yaphe W, Arsenaul GP (1965) Improved resorcinol reagent for the determination of fructose and of 3,6 anhydrogalactose in polysaccharides. Anal Biochem 13:143–148

    Article  CAS  Google Scholar 

  • Zertuche-González JA (1993) Situación actual de la industria de las algas marinas productoras de ficocoloides en México. In: Zertuche-González JA (ed) Situación actual de la industria de macroalgas productoras de ficocoloides en América Latina y el Caribe. FAO Documento de campo No 13

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Dora Luz Arvizu-Higuera .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2007 Springer Science + Business Media B.V.

About this chapter

Cite this chapter

Arvizu-Higuera, D.L., Rodríguez-Montesinos, Y.E., Murillo-Álvarez, J.I., Muñoz-Ochoa, M., Hernández-Carmona, G. (2007). Effect of alkali treatment time and extraction time on agar from Gracilaria vermiculophylla . In: Borowitzka, M.A., Critchley, A.T., Kraan, S., Peters, A., Sjøtun, K., Notoya, M. (eds) Nineteenth International Seaweed Symposium. Developments in Applied Phycology, vol 2. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-9619-8_9

Download citation

Publish with us

Policies and ethics