Algicidal activity of polyunsaturated fatty acids derived from Ulva fasciata and U. pertusa (Ulvaceae, Chlorophyta) on phytoplankton
Isolation of algicidal compounds from Ulva fasciata revealed that the algicidal substances were the polyunsaturated fatty acids (PUFAs) as hexadeca-4,7,10,13-tetraenoic acid (HDTA) C16:4 n-3, octadeca-6,9,12,15-tetraenoic acid (ODTA) C18:4 n-3, α-linolenic acid (ALA) C18:3 n-3 and linoleic acid (LA) C18:2 n-6. The fatty acid composition of four species of Ulvaceae (U. fasciata, U. pertusa, U. arasakii and U. conglobota) was analyzed by capillary gas chromatography to investigate the relationship with the algicidal activity. The results indicate that highly algicidal species, U. fasciata and U. pertusa, showed higher contents of C16:4 n-3, C18:3 n-3, and C18:4 n-3. Concentrations of these PUFAs released from the seaweed in the culture medium were also analyzed. These PUFAs were found to be significantly active against Chattonella antiqua, C. marina, Fibrocapsa japonica, Heterosigma akashiwo, Karenia mikimotoi, moderately effective against Heterocapsa circularisquama, Prorocentrum minimum, P. sigmoides, Scrippsiella trochoidea, whereas low effective against Alexandrium catenella and Cochlodinium polykrikoides. It is suggested that the PUFAs are useful mitigation agents to remove several harmful effects without causing detrimental effects on surrounding marine living organisms.
KeywordsAlgicidal compound Red tide phytoplankton Ulva fasciata Ulva pertusa
Unable to display preview. Download preview PDF.
- Ackman RG, McLachlan J (1977) Fatty acids in some Nova Scotian marine seaweeds: a survey for octadecapentaenoic and other biochemically novel fatty acids. Proc NS Inst Sci 28:47–64Google Scholar
- Imai I, Ishida Y, Sakaguchi K, Hata Y (1995) Algicidal marine bacteria isolated from northern Hiroshima Bay. Fish Sci 61:624–632Google Scholar
- Kamiyama T (2002) Regulation of harmful algal blooms by planktonic ciliates. In: Hiroishi S, Imai I, Ishimaru T (eds) Prevention and extermination strategies of harmful algal blooms. Nihon Suisan Gakkai Kansyu 134, Kouseisya Kouseikaku, Tokyo, Japan, pp 89–101 (in Japanese)Google Scholar
- Kogteva GS, Bezuglov VV (1998) Unsatutared fatty acids as endogenous bioregulators. Biochem (Moscow) 63:6–15Google Scholar
- Murata H, Sakai T, Endo M, Kuroki A, Kimura M, Kumanda K (1989) Screening of removal agents of a red tide plankton Chattonella marina with special reference to the ability of the free radicals derived from the hydrogen peroxide and polyunsaturated fatty acids. Nippon Suisan Gakkaishi 55:1075–1082Google Scholar
- Nakamura Y (2002) Control of red tides by heterotrophic dinoflagellates. In: Hiroishi S, Imai I, Ishimaru T (eds) Prevention and extermination strategies of harmful algal blooms. Nihon Suisan Gakkai Kansyu 134, Kouseisya Kouseikaku, Tokyo, Japan, pp 81–88 (in Japanese)Google Scholar
- Nan C, Zhang H, Zhao G (2004) Allelopathic interactions between the macroalga Ulva pertusa and eight microalgal species. J Seares 52:259–268Google Scholar
- Ryu HY, Shim JM, Bang JD, Lee C (1998) Experimental chemical treatments for the control of dinoflagellate, Cochlodinium polykrikoides in the land-based culture of olive flounder Paralichthys olivaceus. Kor J Aquacult 11:285–294Google Scholar
- Sargent JR, Parkes RJ, Mueller-Harvey I, Henderson J (1987) Lipid biomarkers in marine ecology. In: Sleigh MA (ed) Microbes in the sea. Ellis Horwood, Chichester, United Kingdom, pp 119–138Google Scholar
- Steidinger KA (1983) A re-evaluation of toxic dinoflagellate biology and ecology. Prog Phycol Res 2:147–188Google Scholar
- Takagi T, Asahi M, Itabashi Y (1985) Fatty acid composition of twelve algae from Japanese waters. Yukagaku 34:1008–1012Google Scholar