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Chromatin Condensation

(How Rope is Made from String)

  • Chapter
Apoptotic Chromatin Changes

Summary

The controversy of chromatin condensation resulted in several models for chromosome condensation. However, microscopic methods failed to distinguish among predicted structural motifs. To briefly summarize these developments, the structural organization of chromatin below the metaphase chromosome and beyond the nucleosomal fibril remained unknown. Nucleosomes condense into higher order structures, the nature of which is not elucidated. Due to the stickiness of the nuclear material, decondensed chromatin could not be separated in the interphase nucleus; consequently, the fibrillar chromatin structure could not be analysed. To avoid the stickiness reversible permeabilization was introduced which not only allows the survival of cells, but also permits to open the nucleus any time during the cell cycle. This chapter summarizes general mechanisms that modulate chromatin function, covalent modification of nucleosomal histones, and ATP-dependent remodeling and describes the analysis of chromatin structures in a cell cycle-dependent manner in mammalian and Drosophila cells using high resolution of centrifugal elutriation.

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    Gáspár Bánfalvi (Prof.)

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Bánfalvi, G. (2009). Chromatin Condensation. In: Apoptotic Chromatin Changes. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-9561-0_3

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