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Decomposition Studies Using Animal Models in Contrasting Environments: Evidence from Temporal Changes in Soil Chemistry and Microbial Activity

  • Kathryn L. Stokes
  • Shari L. Forbes
  • Laura A. Benninger
  • David O. Carter
  • Mark Tibbett

Abstract

Traditionally, soil evidence in forensic science has focused predominantly on the transference of soil particles from a victim or suspect and a crime scene. However, a recent increase in forensic taphonomy research has highlighted the potential of soil to provide key information to an investigation involving decomposed remains. A decomposing carcass can release a significant pulse of nutrients into the surrounding soil (gravesoil) resulting in the retention of decomposition products in the soil for a considerable period of time. In order to understand the complex associations between a decomposing carcass and the soil system, research must be conducted in both controlled laboratory environments and outdoor field environments. This chapter discusses two contrasting decomposition studies which aimed to investigate the cadaver/soil interaction. The first study investigated the decomposition of small mouse carcasses buried in soil and was conducted within a controlled laboratory environment in Western Australia. The second study investigated the decomposition of large pig carcasses placed on the soil surface and was conducted in an outdoor field environment in southern Ontario. Both studies investigated a range of decomposition products particularly focusing on carbon-based, nitrogen-based and phosphorus-based compounds as these were considered to offer the most valuable information to address the research questions. The results of both studies provide the opportunity to comment on the effect of carcass size, soil type and decomposition environment on the influx of decomposition products into the soil.

Keywords

Control Soil Loamy Sand Sandy Loam Soil Skeletal Muscle Tissue Soil Microcosm 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Balmelle B, Nguyen KM, Capdeville B, Cornier JC and Deguin A (1992). Study of factors controlling nitrite buildup in biological processes for water nitrification. Water Science and Technology 26:1017–1025.Google Scholar
  2. Brathen KA, Danell K and Berteaux D (2002). Effect of muskox carcasses on nitrogen concentration in tundra. Arctic 55:389–394.Google Scholar
  3. Bray RH and Kurtz LT (1945). Determination of total, organic and available forms of phosphorus in soils. Soil Science 59:39–45.CrossRefGoogle Scholar
  4. Bull PA, Parker A and Morgan RM (2006). The forensic analysis of soils and sediment taken from the cast of a footprint. Forensic Science International 162:6–12.PubMedCrossRefGoogle Scholar
  5. Carter DO and Tibbett M (2006). Microbial decomposition of skeletal muscle tissue (Ovis aries) in a sandy loam soil at different temperatures. Soil Biology and Biochemistry 38:1139–1145.CrossRefGoogle Scholar
  6. Carter DO, Yellowlees D and Tibbett M (2007). Cadaver decomposition in terrestrial ecosystems. Naturwissenschaften 94:12–24.PubMedCrossRefGoogle Scholar
  7. Carter MR (1993). Soil Sampling and Methods of Analysis. CRC, Boca Raton, FL.Google Scholar
  8. Dent BB, Forbes SL and Stuart BH (2004). Review of human decomposition processes in soil. Environmental Geology 45:576–585.CrossRefGoogle Scholar
  9. Freeman JS and Rowell DL (1981). The adsorption and precipitation of phosphate onto calcite. Journal of Soil Science 32:75–84.CrossRefGoogle Scholar
  10. Hoffman DW, Mathews BC and Wicklund RA (1964). Soil associations of southern Ontario, Report No. 30 of the Ontario Soil Survey. Canada Department of Agriculture, Ottawa, Ontario Department of Agriculture, Toronto.Google Scholar
  11. Hopkins DW, Wiltshire PEJ and Turner BD (2000). Microbial characteristics of soils from graves: an investigation at the interface of soil microbiology and forensic science. Applied Soil Ecology 14:283–288.CrossRefGoogle Scholar
  12. Kates M (1986). Techniques of Lipidology: Isolation, Analysis and Identification of Lipids. Elsevier, St Louis, MO.Google Scholar
  13. Kerek M, Drijber RA, Powers WL, Shearman RC, Gaussoin RE and Streich AM (2002). Accumulation of microbial biomass within particulate organic matter of aging golf greens. Agronomy Journal 94:455–461.Google Scholar
  14. Kyveryga PM, Blackmer AM, Ellsworth JW and Isla R (2004). Soil pH effects on nitrification of fall-applied anhydrous ammonia. Soil Science Society America Journal 68:545–551.CrossRefGoogle Scholar
  15. Melis C, Selva N, Teurlings I, Skarpe C, Linnell JDC and Andersen R (2007). Soil and vegetation nutrient response to bison carcasses in Bialowieza Primeval Forest. Poland Journal of Ecological Research 22:807–813.CrossRefGoogle Scholar
  16. Meissner R, Rupp H, Seeger J and Sconert P (1995). Influence of mineral fertilizers and different soil types on nutrient leaching: results of lysimeter studies in east Germany. Land Degradation and Rehabilitation 6:163–170.CrossRefGoogle Scholar
  17. Nugroho RA, Roling WFM, Laverman A and MVerhoef HA (2007). Low nitrification rates in acid Scots pine forest soils are due to pH-related factors. Microbial Ecology 53:89–97.PubMedCrossRefGoogle Scholar
  18. Philips S, Laanbroek HJ and VerstraeteW (2002). Origin, causes and effects of increased nitrite concentrations in aquatic environments. Environmental Science and Biotechnology 1:115–141.CrossRefGoogle Scholar
  19. Putman RJ (1978a). Flow of energy and organic matter from a carcass during decomposition: decomposition of small mammal carrion in temperate systems 2. Oikos 31:58–68.CrossRefGoogle Scholar
  20. Putman RJ (1978b). Patterns of carbon dioxide evolution from decaying carrion: decomposition of small mammal carrion in temperate systems. Oikos 31:47–57.CrossRefGoogle Scholar
  21. Rawlins BG, Kemp SJ, Hodgkinson EH, Riding JB, Vane CH, Poulton C and Freeborough K (2006). Potential and pitfalls in establishing the provenance of earth related samples in forensic investigations. Journal of Forensic Sciences 51:832–845.PubMedCrossRefGoogle Scholar
  22. Rayment GE and Higginson FR (1992). Australian Laboratory Handbook of Soil and Water Chemical Methods. Inkata Press, Melbourne.Google Scholar
  23. Rodriguez WC and Bass WM (1985). Decomposition of buried bodies and methods that may aid in their location. Journal of Forensic Sciences 30:836–852.PubMedGoogle Scholar
  24. Rowell DL (1994). Soil Science Methods and Applications. Addison Wesley Longman, England.Google Scholar
  25. Schoenly KG, Haskell NH, Mills DK, Bieme-Ndi C, Larsen K and Lee Y (2006). Recreating death's acre in the school yard: using pig carcasses as model corpses to teach concepts of forensic entomology and ecological succession. American Biology Teacher 68:402–410.CrossRefGoogle Scholar
  26. Schumacher BA (2002). Methods for the determination of total organic carbon (TOC) in soils and sediments. Report NCEA-C-1282 of the Ecological Risk Assessment Support Centre. United States Environmental Protection Agency, Las Vegas, NV.Google Scholar
  27. Spray CM and Widdowson EM (1950). The effect of growth and development on the composition of mammals. British Journal of Nutrition 4:332–353.PubMedCrossRefGoogle Scholar
  28. Stevenson FJ and Cole MA (1999). Cycles in the Soil (2nd Edition). Wiley, New York.Google Scholar
  29. Tibbett M and Carter DO (Eds.) (2008). Soil Analysis in Forensic Taphonomy: Chemical and Biological Effects of Buried Human Remains. CRC, Boca Raton, FL.Google Scholar
  30. Tibbett M, Carter DO, Haslam T, Major R and Haslam R (2004). A laboratory incubation method for determining the rate of microbial degradation of skeletal muscle tissue in soil. Journal of Forensic Sciences 49:60–565.Google Scholar
  31. Tortora GJ and Grabowski SR (2000). Principles of Anatomy and Physiology (9th Edition). Wiley, New York.Google Scholar
  32. Towne EG (2000). Prairie vegetation and soil nutrient responses to ungulate carcasses. Oecologica 122:232–239.CrossRefGoogle Scholar
  33. Ulen B (1999). Leaching and balances of phosphorus and other nutrients in lysimeters after application of organic manures or fertilizers. Soil Use and Management 15:56–61.Google Scholar
  34. Vadivelu VM, Keller J and Yuan Z (2007). Effect of free ammonia on the respiration and growth processes of an enriched Nitrobacter culture. Water Research 4:826–834.CrossRefGoogle Scholar
  35. van Haaren FWJ (1951). Churchyards as sources for water pollution. Moormans Periodieke Pers 35:167–172 (in Dutch).Google Scholar
  36. Vass AA, Bass WM, Wolt JD, Foss JE and Ammons JT (1992). Time since death determinations of human cadavers using soil solution. Journal of Forensic Sciences 37:1236–1253.PubMedGoogle Scholar
  37. Widdowson EM (1950). Chemical composition of newly born mammals. Nature 166:626–628.PubMedCrossRefGoogle Scholar
  38. Wilson AS, Janaway RC, Holland AD, Dodson HI, Baran E, Pollard AM and Tobin DJ (2007). Modelling the buried human body environment in upland climes using three contrasting field sites. Forensic Science International 169:6–18.PubMedCrossRefGoogle Scholar
  39. Zalba P and Galantini A (2007). Modified soil-test methods for extractable phosphorus in acidic, neutral and alkaline soils. Communication in Soil Science and Plant Analysis 38:1579–1587.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2009

Authors and Affiliations

  • Kathryn L. Stokes
    • 1
  • Shari L. Forbes
    • 2
  • Laura A. Benninger
    • 2
  • David O. Carter
    • 3
  • Mark Tibbett
    • 4
  1. 1.Centre for Land Rehabilitation, School of Earth and Geographical Sciences, Australia, Centre for Forensic Science, University of Western AustraliaCrawleyAustralia
  2. 2.Faculty of ScienceUniversity of Ontario Institute of TechnologyOshawaCanada
  3. 3.Department of EntomologyCollege of Agricultural Sciences and Natural resources, University of Nebraska — LincolnLincolnUSA
  4. 4.Centre for Land Rehabilitation, School of Earth and Geographical Sciences, University of Western AustraliaCrawleyAustralia

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