Microbial Response to Charcoal Amendments and Fertilization of a Highly Weathered Tropical Soil

Charcoal is a major component of stable SOM in terras pretas alsocalled Amazonian Dark Earths (Glaser et al. 2001a, b; Glaser 2007). Apart from charcoal, special microbes could contribute to the formation of the highlystable SOM in terra preta (Woods and McCann 1999). However, this is stillmatter of speculation. There could be a link between the high amounts of charcoal in terra preta soils and soil microbial community composition (Glaser 2007). Although it is unlikely that charcoal is used by microorganisms as a direct carbon source, habitat properties are certainly different with the presence or absenceof charcoal (Saito and Marumoto 2002). Steiner et al. (2004) could demonstrate that charcoal addition to Ferralsols significantly increased microbial activity. Charcoal also promoted the colonization of agricultural plants with arbuscular mycorrhizal fungi (Nishio 1996; Saito and Marumoto 2002), it improved nodule weight (Nishio 1996) and nitrogen fixation (Tryon 1948; Nishio 1996; Rondon et al. 2007).

Most soil microorganisms cannot be characterized by conventional cultivation techniques (Zelles 1999). Amann et al. (1995) estimated that 80–99% of all micro-bial species have not yet been cultured. Therefore, biomarkers such as ribosomal nucleic acids (RNA) or phospholipid fatty acids (PLFA) are better analytical tools to provide an unbiased view on the structure of complex soil microbial communities (Zelles 1999). PLFA are exclusively found in the membranes of living organisms and comprise a relatively constant portion of living biomass within a microbial community (Zelles 1999). The analysis of PLFA allows the simultaneous quantification of microbial biomass and the characterization of the microbial community structure by specific biomarker PLFA (Tunlid and White 1992; Zelles 1999; Gattinger 2001).

Keywords

Biomass Combustion Phosphorus Magnesium Titration 

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References

  1. Amann R, Ludwig W, Schleifer KH (1995) Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiological Review 59: 143–146Google Scholar
  2. Baath E, Anderson TH (2003) Comparison of soil fungal/bacterial ratios in a pH gradient using physiological and PLFA-based techniques. Soil Biology & Biochemistry 35(7): 955–963CrossRefGoogle Scholar
  3. Burke RA, Molina M, Cox JE, Osher LJ, Piccolo MC (2003) Stable carbon isotope ratio and composition of microbial fatty acids in tropical soils. Journal of Environmental Quality 32(1): 198–206Google Scholar
  4. Calderon FJ, Jackson LE, Scow KM, Rolston DE (2001) Short-term dynamics of nitrogen, micro-bial activity, and phospholipid fatty acids after tillage”. Soil Science Society of America Journal 65(1): 118–126CrossRefGoogle Scholar
  5. FAO (1990) Soil Map of the World, Revised Legend. FAO, Rome, ItalyGoogle Scholar
  6. Frostegard A, Baath E, Tunlid A (1993) Shifts in the structure of soil microbial communities in limed forests as revealed by phospholipid fatty acid analysis. Soil Biology & Biochemistry 25(6): 723–730CrossRefGoogle Scholar
  7. Frostegard A, Baath E (1996) The use of phospholipid fatty acid analysis to estimate bacterial and fungal biomass in soil. Biology and Fertility of Soils 22(1/2): 59–65CrossRefGoogle Scholar
  8. Frostegard A, Tunlid A, Baath E (1991) Microbial biomass measured as total lipid phosphate in soils of different organic content. Journal of Microbiological Methods 14(3): 151–163CrossRefGoogle Scholar
  9. Gattinger A (2001) Entwicklung und Anwendung von Methoden zur Charakterisierung von mikrobiellen Gemeinschaften in oxischen und anoxischen Bodenökosystemen anhand von Phospholipid-Profilen. Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt. Weihenstephan, Technischen Universität München: 147Google Scholar
  10. Glaser B (2007) Prehistorically modified soils of central Amazonia: a model for sustainable agriculture in the 21st century? Philosophical Transactions of the Royal Society of London Series B 362: 187–196CrossRefGoogle Scholar
  11. Glaser B, Birk J, Steiner C, Texeira WG (in preperation) Microbial utilization of labile carbon under charcoal, inorganic, and organic fertilizationGoogle Scholar
  12. Glaser B, Guggenberger G, Zech W (2001a) Black carbon in sustainable soils of the brazilian Amazon region. In: Swift RS, Spark KM (eds) Understanding and Managing Organic Matter in Soils, Sediments and Waters. St. Paul, MN, International Humic Substances Society, pp 359–364Google Scholar
  13. Glaser B, Haumaier L, Guggenberger G, Zech W (2001b) The “Terra Preta” phenomenon: a model for sustainable agriculture in the humid tropics. Naturwissenschaften 88(1): 37–41CrossRefGoogle Scholar
  14. Hedlund K (2002) Soil microbial community structure in relation to vegetation management on former agricultural land. Soil Biology & Biochemistry 34(9): 1299–1307CrossRefGoogle Scholar
  15. Knapp DR (1979) Handbook of Analytical Derivatization Reactions. New York, WileyGoogle Scholar
  16. Mehlich A (1984) Mehlich-3 Soil test extractant: a modification of Mehlich-2 extractant. Communications in Soil Science and Plant Analysis 15:1409–1416CrossRefGoogle Scholar
  17. Nishio M (1996) Microbial fertilizers in Japan, FFTC-Extension Bulletins 1–12. National Institute of Agro-Environmental Science, Ibaraki, JapanGoogle Scholar
  18. Olsson PA (1999) Signature fatty acids provide tools for determination of the distribution and interactions of mycorrhizal fungi in soil. Fems Microbiology Ecology 29(4): 303–310CrossRefGoogle Scholar
  19. Olsson PA, Francis R, Read DJ, Soderstrom B (1998) Growth of arbuscular mycorrhizal mycelium in calcareous dune sand and its interaction with other soil microorganisms as estimated by measurement of specific fatty acids. Plant and Soil 201(1): 9–16CrossRefGoogle Scholar
  20. Rondon MA, Lehmann J, Ramírez, Hurtado M (2007) Biological nitrogen fixation by common beans (Phaseolus vulgaris L.) increases with bio-char additions. Biology and Fertility of Soils, (291):275–290Google Scholar
  21. Ruess L, Haggblom MM, Garcia Zapata EJ, Dighton J (2002) Fatty acids of fungi and nematodes — possible biomarkers in the soil food chain? Soil Biology & Biochemistry 34(6): 745–756CrossRefGoogle Scholar
  22. Saito M, Marumoto T (2002) Inoculation with arbuscular mycorrhizal fungi: the status quo in Japan and the future prospects. Plant and Soil 244(1–2): 273–279CrossRefGoogle Scholar
  23. Sakamoto K, Iijima T, Higuchi R (2004) Use of specific phospholipid fatty acids for identifying and quantifying the external hyphae of the arbuscular mycorrhizal fungus Gigaspora rosea. Soil Biology & Biochemistry 36(11): 1827–1834CrossRefGoogle Scholar
  24. Steinberger Y, Zelles L, Bai QY, von Lutzow M, Munch JC (1999) Phospholipid fatty acid profiles as indicators for the microbial community structure in soils along a climatic transect in the Judean Desert. Biology and Fertility of Soils 28(3): 292–300CrossRefGoogle Scholar
  25. Steiner C, Teixeira WG, Lehmann J, Zech W (2004) Microbial response to charcoal amendments of highly weathered soils and Amazonian Dark Earths in Central Amazonia-preliminary results. In: Glaser B, Woods WI (eds) Amazonian Dark Earths: Exploration in Space and Time. Springer, Berlin/Heidelberg/New York, pp 195–212Google Scholar
  26. Treonis AM, Ostle NJ, Stott AW, Primrose R, Grayston SJ, Ineson P (2004) Identification of groups of metabolically-active rhizosphere microorganisms by stable isotope probing of PLFAs. Soil Biology & Biochemistry 36(3): 533–537CrossRefGoogle Scholar
  27. Tryon EH (1948) Effect of charcoal on certain physical, chemical, and biological propertieson forest soils. Ecological Monographs 18: 82–115CrossRefGoogle Scholar
  28. Tunlid A, White DC (1992) Biochemical analysis of biomass, community structure, nutritional status, and metabolic activity of microbial communities in soil. Soil Biochemistry 7: 229–262Google Scholar
  29. van Aarle IM, Olsson PA (2003) Fungal lipid accumulation and development of mycelial structures by two Arbuscular Mycorrhizal Fungi. Applied and Environmental Microbiology 69(11): 6762–6767CrossRefGoogle Scholar
  30. Waldrop MP, Balser TC, Firestone MK (2000) Linking microbial community composition to function in a tropical soil. Soil Biology & Biochemistry 32(13): 1837–1846CrossRefGoogle Scholar
  31. Wood M (1995) The role of bacteria and actinomycetes in litter decomposition in the tropics. Soil organisms and litter decomposition in the tropics. M. V. Reddy. Westview Press, Boulder, CO, pp 13–38Google Scholar
  32. Woods WI, McCann JM (1999) The anthropogenic origin and persistence of Amazonian dark earths. The Yearbook of the Conference of Latin American Geographers 25. Austin: University of Texas Press, 7–14Google Scholar
  33. Zelles L (1997) Phospholipid fatty acid profiles in selected members of soil microbial communities. Chemosphere 35: 275–294CrossRefGoogle Scholar
  34. Zelles L (1999) Fatty acid patterns of phospholipids and lipopolysaccharides in the characterization of microbial communities in soil: a review. Biology and Fertility of Soils 29(2): 111–129CrossRefGoogle Scholar

Copyright information

© Springer Science + Business Media B.V 2009

Authors and Affiliations

  1. 1.Institute of Soil Science and Soil GeographyUniversity of BayreuthBayreuthGermany
  2. 2.Biorefining and Carbon Cycling Program, Department of Biological and Agricultural EngineeringUniversity of GeorgiaAthensUSA

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