Improving Formulation of Biocontrol Agents Manipulating Production Process

  • J. Usall
  • N. Teixidó
  • M. Abadias
  • R. Torres
  • T. Cañamas
  • I. Viñas
Chapter
Part of the Plant Pathology in the 21st Century book series (ICPP, volume 2)

Abstract

There are several reasons of the limited number of commercial available biocontrol agents, such as the difficulties in developing a shelf-stable formulated product that retains biocontrol activity. This chapter shows that it is possible to improve the formulations during the production process and describes several examples of improving liquid and dry formulations using different strategies such as grow microorganisms in a w modified media, under sublethal thermal stress conditions or preservation in isotonic solutions.

Liquid formulation of C. sake was improved growing the cells in molasses medium with a w modified to 0.98 with the addition of sorbitol and preserved with an isotonic trehalose solution. After 180 days of storage at 4°C, the viability of this formulate was 100% and the efficacy against Penicillium expansum on apples was more than 95% rot reduction.

Spray drying formulations were improved by modifying growth media or temperatures during growing period. The biocontrol agent Pantoea agglomerans grown during 48 h in NaCl 0.97 a w modified medium could increase their viability after spray drying formulation from 6% in unmodified medium to near 30% without affecting their biocontrol potential.

In contrast Candida sake cells grown in unmodified molasses medium exposed to mild heat treatments at 30°C or 33°C during mid or late-exponential or early or mid-stationary growth phases showed an increase of survival when are exposed to lethal shock at 40°C, but only a very reduced improvement after spray drying formulation.

Finally the combination of thermal and osmotic stress was studied in order to improve fluidized bed drying formulations of P.agglomerans. The results showed than using NaCl to adjust a w to 0.988 in the growth medium and increasing the temperature to 35°C during 1 h in the early stationary phase could get a good formulate with only 0.5 log reductions during fluidized bed drying process.

Keywords

Liquid formulation spray drying thermal stress fluidized bed drying osmotic stress isotonic solutions candida sake pantoea agglomerans 

Notes

Acknowledgements

The authors are grateful to Spanish government (Ministerio de Ciencia y Tecnología) for grants AGL-2002-01137 and AGL-2005-02510 and to FEDER (Fondo Europeo de Desarrollo Regional) for their financial support.

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • J. Usall
    • 1
  • N. Teixidó
    • 1
  • M. Abadias
    • 1
  • R. Torres
    • 1
  • T. Cañamas
    • 1
  • I. Viñas
    • 2
  1. 1.IRTA, UdL-IRTA Centre, XaRTA-PostharvestLleidaCatalonia
  2. 2.University of Lleida, UdL-IRTA Centre, XaRTA-PostharvestLleidaCatalonia

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