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The Role of Pre-formed Antifungal Substances in the Resistance of Fruits to Postharvest Pathogens

  • Nimal Adikaram
  • Chathurika Karunanayake
  • Charmalie Abayasekara
Chapter
Part of the Plant Pathology in the 21st Century book series (ICPP, volume 2)

Abstract

Plants contain secondary metabolites with antifungal properties. In fruits they are mostly concentrated in the peel at immature stage and decline during ripening in coincidence with fungal rot development. The information on antifungal systems in immature avocado and mango, reviewed here, suggests that they play a role in natural disease resistance. Immature mangoes have evolved a formidable antifungal system comprising several resorcinols, gallotannins and chitinases. Resorcinols and gallotannins are inhibitory to major postharvest pathogens, Colletotrichum gloeosporioides causing anthracnose and Botryodiplodia theobromae causing stem-end rot. Their levels are generally higher in resistant cultivars than in susceptible ones. Mango latex, distributed in a fine network of canals in the fruit peel, contains chitinases which have the ability to rapidly digest conidia of C. gloeosporioides. Gallotannins and resorcinols decline progressively during ripening and the latex disappears when ripe rot development begins. Retention of latex in the harvested fruit reduces anthracnose and stem-end rot development during ripening. Treatment of harvested fruit with CO2 or inoculation with certain non-pathogenic fungi increased antifungal resorcinol concentration. Immature avocado fruits possess a pre-formed antifungal system comprising at least five antifungal compounds. The quiescence of C. gloeosporioides in the immature fruit has been attributed to the pre-formed antifungal activity of the peel. Lipoxygenase activity increases during fruit ripening, while epicatechin levels decline, suggesting that these events are linked to the decrease in di-ene concentrations. Inhibition of lipoxygenase activity results in retention of antifungal di-ene during ripening increasing fruit resistance. In freshly harvested avocados, the di-ene concentration can be further enhanced by treatment with biotic and abiotic agents.

Keywords

Pre-formed antifungal compounds resorcinols gallotannins chitinase mango 

References

  1. Adikaram NKB, Brown AE, Swinburne TR (1982) Phytoalexin involvement in the latent infection of Capsicum annuum L. fruit by Glomerella cingulata (Stonem.). Physiol Plant Pathol 21:161-170CrossRefGoogle Scholar
  2. Adikaram NKB, Ewing DF, Karunaratne AM, Wijeratne EMK (1992) Antifungal compounds from immature avocado fruit peel. Phytochemistry 31(1):93-96CrossRefGoogle Scholar
  3. Ardi R, Kobiler I, Jacoby B, Keen NT, Prusky D (1998) Involvement of epicatechin biosynthesis in the activation of the mechanism of resistance of avocado fruits to Colletotrichum gloeosporioides. Physiol Mol Plant Pathol 53:269-285CrossRefGoogle Scholar
  4. Bandyopadhyay C, Gholap AS, Mamdapur VR (1985) Characterization of alkenylresorcinols in mango (Mangifera indica L.) latex. J Agr Food Chem 33:377-379CrossRefGoogle Scholar
  5. Berardini N, Carle R, Schieber A (2004) Characterization of gallotannins and benzophenone derivatives from mango (Mangifera indica L. cv. Tommy Atkins) peel, pulp and kernels by high performance liquid chromatography/electrospray ionization mass spectrometry. Rapid Comm Mass Spectrom 18:2208-2216CrossRefGoogle Scholar
  6. Binyamini N, Schiffmann-Nadel M (1972) Latent infection in avocado fruit due to Colletotrichum gloeosporioides. Phytopathology 67:315-320Google Scholar
  7. Coates LM, Muirhead IF, Irwin JAG, Gowanlock DH (1993) Initial infection process by Colletotrichum gloeosporioides. Mycol Res 97(1):1363-1370CrossRefGoogle Scholar
  8. Cojocaru M, Droby S, Glotter E, Goldman A, Gottlieb HE, Jacoby B, Prusky D (1986) 5-(12-heptadecenyl)-resorcinol, the major component of the antifungal activity in the peelof mango fruit. Phytochemistry 25(5):1093-1095CrossRefGoogle Scholar
  9. Droby S, Prusky D, Jacoby B, Goldman A (1986) Presence of antifungal compounds in the peel of mango fruits and their relation to latent infections of Alternaria alternate. Physiol Mol Plant Pathol 29:173-183CrossRefGoogle Scholar
  10. Droby S, Prusky D, Jacoby B, Goldman A (1987) Induction of antifungal resorcinols in unripe mango fruits and its relation to latent infection by Alternaria alternata. Physiol Mol Plant Pathol 30:285-292CrossRefGoogle Scholar
  11. Hall R (1971) Pathogenicity of Monilinia fructicola. Part II. Penetration of peach leaf and fruit. Phytopathologische zeitschrift 72:281-290CrossRefGoogle Scholar
  12. Hassan KM (2006) Constitutive alk(en)ylresorcinols and resistance to postharvest diseases in mango (Mangifera indica L.). Ph.D. thesis, University of Queensland, Australia, pp 229Google Scholar
  13. Hassan MK, Dann EK, Irving DE, Coates LM (2007) Concentrations of constitutive alk(en)ylresorcinols in peel of commercial mango varieties and resistance to postharvest anthracnose. Physiol Mol Plant Pathol 71:158-165CrossRefGoogle Scholar
  14. Kabuki T, Nakajima H, Arai M, Ueda S, Kawabara Y, Dosako S (2000) Characterization of navel antimicrobial compounds from mango (Mangifera indica) kernal seeds. Food Chem 71:61CrossRefGoogle Scholar
  15. Karunanayake KOLC (2008) Natural defence mechanisms in mango fruit and their potential in the management of postharvest diseases. Ph.D. Thesis, University of Peradeniya, Sri Lanka, pp 297Google Scholar
  16. Knodler M, Berardini N, Kammerer RD, Carle R, Sciber A (2007) Characterization of major and minor alk(en)yl resorcinols from mango (Mangifera indica L.) peels by high-performance liquid chromatography/atomic pressure chemical ionization mass spectrometry. Rapid Comm Mass Spectrom 21:945-951CrossRefGoogle Scholar
  17. Kobiler I, Reved R, Artez L, Prusky D (1998) Antifungal compounds regulating postharvest diseases in mango. In: Johnson GI, Highley E, Joyce DC (eds) Disease resistance in fruit. ACIAR Proceedings No. 80, 109-114Google Scholar
  18. Mayer AM (1987) Polyphenol oxidases in plants - recent progress. Phytochemistry 26(1):11-20CrossRefGoogle Scholar
  19. Muirhead IF, Deverall BJ (1981) Role of appressoria in latent infection of banana fruits by Colletotrichum musae. Physiol Plant Pathol 19:77-84Google Scholar
  20. Oka K, Saito F, Yasuhara T, Sugimoto A (2004) A study of cross-reactions between mango contact allergens and urushiol. Contact Dermatitis 51:292-296CrossRefPubMedGoogle Scholar
  21. Osbourn AE (1996) Saponins and plant defence - a soap story. Trends Plant Sci 1:4-9CrossRefGoogle Scholar
  22. Prusky D, Keen NT (1993) Involvement of preformed antifungal compounds in the resistance of subtropical fruits to fungal decay. Plant Dis 77:114-119CrossRefGoogle Scholar
  23. Prusky D, Keen NT (1995) Inducible preformed compounds and their involvement in the resistance of plants to pathogens. In: Reuveni R (ed) Novel approaches to integrated pest management. Lewis Publishers, Boca Raton, FL, pp 139-151Google Scholar
  24. Prusky D, Keen NT, Sims JJ, Midland SL (1982) Possible involvement of an antifungal diene in the latency of Colletotrichum gloeosporioides on unripe avocado fruit. Phytopathology 72:1578-1582CrossRefGoogle Scholar
  25. Prusky D, Keen NT, Eaks I (1983) Further evidence for the involvement of a pre-formed antifungal compound in the latency of Colletotrichum gloeosporioides on unripe avocado fruits. Physiol Mol Plant Pathol 22:189-198Google Scholar
  26. Prusky D, Kobiler I, Jacoby B, Sims JJ, Midland SL (1985) Inhibitors of avocado lipoxygenase; their possible relationship with the latency of Colletotrichum gloeosporioides. Physiol Mol Plant Pathol 27:269-279CrossRefGoogle Scholar
  27. Prusky D, Karni L, Kobiler L, Plumbley RA (1990) Induction of the antifungal diene in unripe avocado fruits: effect of inoculation with Colletotrichum gloeosporioides. Physiol Mol Plant Pathol 37:425-435CrossRefGoogle Scholar
  28. Prusky D, Kobiler I, Fishman Y, Sims JJ, Midland SL, Keen NT (1991) Identification of an antifungal compound in unripe avocado fruits and its possible involvement in the quiescent infections of Colletotrichum gloeosporioides. J Phytopathol 132:319-327CrossRefGoogle Scholar
  29. Prusky D, Freeman S, Rodriguez R, Keen NT (1994) A nonpathogenic mutant strain of Colletotrichum magna induces resistance to Colletotrichum gloeosporioides in avocado fruits. Mol Plant Microbe Interact 7:326-333Google Scholar
  30. Prusky D, Wattad C, Kobiler I (1996) Effect of ethylene on the activation of quiescent infection of Colletotrichum gloeosporioides in avocado fruits. Mol Plant Microbe Interact 9:864-868Google Scholar
  31. Schonbeck F, Schlosser E (1976) Preformed substances as potential protectants. In: Heitefuss R, Williams PH (eds) Physiological Plant Pathology. Springer-Verlag, Berlin, pp 653-678Google Scholar
  32. Sivanathan S, Adikaram NKB (1989) Biological activity of four antifungal compounds in immature avocado. J Phytopathol 125:97-109CrossRefGoogle Scholar
  33. VanEtten HD, Mansfield JW, Bailey JA, Farmer EE (1994) Letter to the editor. Two classes of plant antibiotics: phytoalexins versus phytoanticipins Plant Cell 6:1191-1192Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Nimal Adikaram
    • 1
  • Chathurika Karunanayake
    • 1
  • Charmalie Abayasekara
    • 1
  1. 1.Department of BotanyUniversity of PeradeniyaPeradeniyaSri Lanka

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