Abstract
In oat (Avena sativa L.) low production rates have limited the use of doubled haploids (DH). Alternative techniques for oat DH production are anther culture and wide hybridization. In this paper, progress in anther culture is summarized, concentrating specially on the work done in MTT Agrifood Research Finland. Up to 30 green plants per 100 isolated anthers have been regenerated from cultivated oat, but common recoveries are much below that. Genotype-dependency is also evident. Oat anther culture can be used to produce DH populations for genetic mapping, as well as material for other genetic research purposes. Application to the practical cultivar breeding may still demand improved DH production efficiency, as well as overcome of the genotypic restrictions.
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References
Bjørnstad Å, Opsahl H-G, Aasmo M (1989) Effects of donor plant environment and light during incubation on anther cultures of some spring wheat (Triticum aestivum L.) cultivars. Plant Cell Tiss Org Cult 17:27–37
Forster BP, Thomas WTB (2005) Doubled haploids in genetics and plant breeding. Plant Breed Rev 25:57–88
Immonen S, Tauriainen A, Manninen O (1999) Assesment of green regenerants from rye and triticale anther cultures. In: Clement C, Pacini E, Audran JC (eds) Anther and pollen: from biology to biotechnology. Springer, Berlin, pp. 237–245
Hu H (1997) In vitro induced haploids in wheat. In: Jain SM, Sopory SK, Veilleux RE (eds) In vitro haploid production in higher plants, Vo l 4: Cereals. Kluwer, Dordrecht, pp. 73–97
Kiviharju E, Pehu E (1998) The effect of cold and heat pretreatments on anther culture response of Avena sativa and A. sterilis. Plant Cell Tiss Org Cult 54:97–104
Kiviharju E, Tauriainen A (1999) 2,4-dichlorophenoxyacetic acid and kinetin in anther culture of cultivated and wild oats and their interspecific crosses: plant regeneration from Avena sativa L. Plant Cell Rep 18:582–588
Kiviharju E, Puolimatka M, Pehu E (1997) Regeneration of anther-derived plants of Avena sterilis. Plant Cell Tiss Org Cult 48:147–152
Kiviharju E, Puolimatka M, Saastamoinen M, Hovinen S, Pehu E (1998) The effect of genotype on anther culture response of cultivated and wild oats. Agric Food Sci Finl 7:409–422
Kiviharju E, Puolimatka M, Saastamoinen M, Pehu, E (2000) Extension of anther culture to several genotypes of cultivated oats. Plant Cell Rep 19:674–679
Kiviharju E, Laurila J, Lehtonen M, Tanhuanpää P, Manninen O (2004) Anther culture properties of oat × wild red oat progenies and a search for RAPD markers associated with anther culture ability. Agric Food Sci 13:151–162
Kiviharju E, Moisander S, Laurila J (2005) Improved green plant regeneration rates from oat anther culture and the agronomic performance of some DH lines. Plant Cell Tiss Org Cult 81:1–9
Ma H, Busch RH, Riera-Lizarazu O, Rines HW, Dill-Macky R (1999) Agronomic performance of lines derived from anther culture, maize pollination and single-seed descent in a spring wheat cross. Theor Appl Genet 99:432–436
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue culture. Physiol Plant 15:437–497
Ouédraogo JT, St-Pierre C-A, Collin J, Rioux S, Comeau A (1998) Effect of amino acids, growth regulators and genotype on androgenesis in barley. Plant Cell Tiss Org Cult 53:59–66
Ouyang JW, Jia SE, Zhang C, Chen X, Feng G (1989) A new synthetic medium (W14) for wheat anther culture. Annu Rep Inst Genet Acad Sin 1987–1988, Beijing, pp. 91–92
Powell W, Thomas WTB, Thompson DM (1992) The agronomic performance of anther culture derived plants of barley produced via pollen embryogenesis. Ann Appl Biol 120:137–150
Rines HW (1983) Oat anther culture: genotype effect on callus initiation and the production of a haploid plant. Crop Sci 23:268–272
Rines HW (2003) Oat haploids from wide hybridization. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled Haploid Production in Crop Plants. Kluwer, Dordrecht, pp. 155–159
Rines HW, Riera-Lizarazu O, Nunez VM, Davis DW, Phillips RL (1997) Oat haploids from anther culture and from wide hybridizations. In: Jain SM, Sopory SK, Veilleux RE (eds) In Vitro Haploid Production in Higher Plants, Vol. 4. Kluwer, Dordrecht, pp. 205–221
Sidhu PK, Howes NK, Aung T, Zwer PK, Davies PA (2006) Factors affecting oat haploid production following oat × maize hybridization. Plant Breed 125:243–247
Ślusarkiewicz-Jarzina A, Ponitka A (2007) The effect of physical medium state on anther culture response in polish cultivated oat (Avena sativa L.). Acta Biol Craciviensia Ser Bot 49:27–31
Sun CS, Lu T-G, Söndahl MR (1991) Anther culture of naked oat and the establishment of its haploid suspension cell. Acta Bot Sin 33:417–420
Torp AM, Hansen AL, Andersen SB (2001) Chromosomal regions associated with green plant regeneration in wheat (Triticum aestivum L.) anther culture. Euphytica 199:377–387
Zhou H (1996) Genetics of green plant regeneration from anther culture in cereals. In: Jain SM, Sopory SK, Veilleux RE (eds) In vitro haploid production in higher plants, Vol. 2: Applications. Kluwer, Dordrecht, pp. 169–187
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Kiviharju, E.M. (2009). Anther Culture Derived Doubled Haploids in Oat. In: Touraev, A., Forster, B.P., Jain, S.M. (eds) Advances in Haploid Production in Higher Plants. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8854-4_14
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DOI: https://doi.org/10.1007/978-1-4020-8854-4_14
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