Abstract
Decorin is a small leucine-rich repeat proteoglycan linked to a single chondroitin or dermatan sulfate (CS/DS) glycosaminoglycan (GAG) chain depending on tissue expressed. Determination of molecular characteristics of CS/DS, is essential for understanding decorin functions. CS/DS analysis includes the identification of glycoforms obtained after detachment from coreprotein followed by collecting information upon epimerization, sulfate content and its distribution. For this purpose, one of the most efficient methods is electrospray ionization (ESI) tandem mass spectrometry (MS/MS). Here we present an approach for structural investigation of human skin decorin CS disaccharides based on combining a specific enzyme digestion with negative ion mode ESI MS-screening followed by fragmentation analysis by collision-induced dissociation (CID) MS/MS. The obtained results showed clearly that human decorin encompasses oversulfated CS motifs in which the site of oversulfation is at glucuronic acid. Moreover, it was possible to demonstrate that the presence of regular and/or irregular GAG domains, which play a critical biological role, can be directly documented by ion sequencing in tandem MS experiments.
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References
Berg JM, Tymoczko JL, Stryer L. Biochemistry 6-th Ed., W.H. Freeman and Company, 2007, New York.
Seidler DG, Peter-Kataliniü J, Zamfir AD. Galactosaminoglycan function and oligosaccharide structure determination. Sci World J. 2007, 7, 233-241.
Iozzo RV. Matrix proteoglycans: from molecular design to cellular function. Annu Rev Biochem. 1998, 67, 609-652.
Charni-Ben Tabassi N, Garnero P. Monitoring cartilage turnover. Curr Rheumatol Rep. 2007, 25, 16-24.
Zamfir AD, Seidler DG, Kresse H, Peter-Kataliniü J. Structural investigation of chondroitin/ dermatan sulfate oligosaccharides from human skin fibroblast decorin. Glycobiology. 2003, 13, 733-742.
Rühland C, Schönherr E, Robenek H, Hansen U, Iozzo RV, Bruckner P, Seidler DG. The glycosaminoglycan chain of decorin plays an important role in collagen fibril formation at the early stages of fibrillogenesis. FEBS J. 2007, 274, 4246-4255.
Zutter MM, Edelson BT. The alpha2beta1 integrin: a novel collectin/C1q receptor. Immuno-biology. 2007, 212, 343-353.
Zanotti S, Saredi S, Ruggieri A, Fabbri M, Blasevich F, Romaggi S, Morandi L, Mora M. Altered extracellular matrix transcript expression and protein modulation in primary Duchenne muscular dystrophy myotubes. Matrix Biol. 2007, 26, 615-624.
Zhu J, Li Y, Shen W, Qiao C, Ambrosio F, Lavasani M, Nozaki M, Branca MF, Huard J. Relationships between transforming growth factor-beta1, myostatin, and decorin: implica-tions for skeletal muscle fibrosis. J Biol Chem. 2007, 282, 25852-25863.
Zhang Z, Li XJ, Liu Y, Zhang X, Li YY, Xu WS. Recombinant human decorin inhibits cell proliferation and downregulates TGF-beta1 production in hypertrophic scar fibroblasts. Burns. 2007, 33, 634-641.
Schnabel LV, Mohammed HO, Miller BJ, McDermott WG, Jacobson MS, Santangelo KS, Fortier LA. Platelet rich plasma (PRP) enhances anabolic gene expression patterns in flexor digitorum superficialis tendons. J Orthop Res. 2007, 25, 230-240.
Shi YF, Zhang Q, Cheung PY, Shi L, Fong CC, Zhang Y, Tzang CH, Chan BP, Fong WF, Chun J, Kung HF, Yang M. Effects of rhDecorin on TGF-beta1 induced human hepatic stel-late cells LX-2 activation. Biochim Biophys Acta. 2006, 1760, 1587-1595.
Goldoni S, Iozzo RA, Kay P, Campbell S, McQuillan A, Agnew C, Zhu JX, Keene DR, Reed CC, Iozzo RV. A soluble ectodomain of LRIG1 inhibits cancer cell growth by attenuat-ing basal and ligand-dependent EGFR activity. Oncogene. 2007, 26, 368-381.
Nash MA, Loercher AE, Freedman RS. In vitro growth inhibition of ovarian cancer cells by decorin: synergism of action between decorin and carboplatin. Cancer Res. 1999, 59, 6192-6196.
Seidler DG, Breuer E, Grande-Allen KJ, Hascall VC, Kresse H. Core protein dependence on epimerization of glucuronosyl residues in galactosaminoglycans. J Biol Chem. 2002, 277, 42409-42416.
Zaia J. Mass spectrometry of oligosaccharides. Mass Spectrom Rev. 2004, 23, 161-227.
Miller MJ, Costello CE, Malmstrom A, Zaia J. A tandem mass spectrometric approach to de-termination of chondroitin/dermatan sulfate oligosaccharide glycoforms. Glycobiology. 2006, 16, 502-513.
Desaire H, Leary JA. Detection and quantification of the sulfated disaccharides in chondro-itin sulfate by electrospray tandem mass spectrometry, J Am Soc Mass Spectrom. 2000, 11, 916-920.
Henriksen J, Hoffmeyer Ringborg L, Roepstorff P. On-line size-exclusion chromatography/ mass spectrometry of low molecular mass heparin. J Mass Spectrom. 2004, 39, 1305-1312.
Hitchcock AM, Costello CE, Zaia J. Glycoform quantification of chondroitin/dermatan sul-fate using a liquid chromatography-tandem mass spectrometry platform. Biochemistry. 2006, 45, 2350-2361.
Zamfir AD, Peter-Kataliniü J. Capillary electrophoresis-mass spectrometry for glycoscreening in biomedical research. Electrophoresis. 2004, 25, 1949-1963.
Zamfir AD, Seidler DG, Kresse H, Peter-Kataliniü J. Structural characterization of chondroitin/ dermatan sulfate oligosaccharides from bovine aorta by capillary electrophoresis and electro-spray ionization quadrupole time-of-flight tandem mass spectrometry. Rapid Commun Mass Spectrom. 2002, 16, 2015-2024.
Zamfir AD, Seidler DG, Kresse H, Peter-Kataliniü J. Structural investigation of chondro-itin/dermatan sulfate oligosaccharides from human skin fibroblast decorin. Glycobiology. 2003, 13, 733-742.
Wolff JJ, Chi L, Linhardt RJ, Amster IJ. Distinguishing glucuronic from iduronic acid in glycosaminoglycan tetrasaccharides by using electron detachment dissociation. Anal Chem. 2007, 79, 2015-2022.
Domon B, Costello CE. A systematic nomenclature for carbohydrate fragmentations in FAB-MS/MS spectra of glycoconjugates. Glycoconjugate J. 1988, 5, 397-409.
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Muresan, A., Galusca, M., Seidler, D.G., Dinca, N., Zamfir, A.D. (2008). Structural Analysis of Chondroitin Sulfate Disaccharides by Electrospray Ionization High Capacity Ion Trap Mass Spectrometry. In: Popescu, C., Zamfir, A.D., Dinca, N. (eds) Applications of Mass Spectrometry in Life Safety. NATO Science for Peace and Security Series A: Chemistry and Biology. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8811-7_6
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DOI: https://doi.org/10.1007/978-1-4020-8811-7_6
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