Abstract
Verticillium wilt is one of the most serious soilborne diseases worldwide. Three non-fumigant control methods that appear to have great potential for reducing losses due to wilt and other soilborne pathogens are detailed here. High nitrogen organic amendments and products containing volatile fatty acids (VFAs) can significantly reduce disease severity and inoculum density but only under specific soil conditions. Identification of the modes of action for these products provides new avenues to improve their efficacy. Broccoli amendments also effectively reduce Verticillium wilt and have great potential for use on a large scale where economics allow. Grafting susceptible cultivars onto Verticillium resistant root stocks has become widely adopted in many countries. Eggplants and tomatoes provide a good model system for testing this technology. Promising results have been obtained under diverse disease pressure and soil and climatic conditions.
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References
Abbasi PA, Lazarovits G, Jabaji-Hare S (2009) Detection of high concentrations of organic acids in fish emulsion and their role in pathogen or disease suppression. Phytopathology 99:274–281
Abbasi PA, Conn KL, Lazarovits G (2006) Effect of fish emulsion used as a pre-plant soil amendment on Verticillium wilt, common scab, and tuber yield of potato. Can J Plant Pathol 28:509–518
Abbasi PA, Conn KL, Lazarovits G (2007) Managing soilborne diseases of vegetable crops with a pre-plant soil or substrate amendment of a corn distillation product. Biocontrol Sci Tech 17:331–344
Bailey KL, Lazarovits G (2003) Suppressing soilborne diseases with residue management and organic amendments. Soil Tillage Res 72:169–180
Barbara DJ, Clewes E (2003) Plant pathogenic Verticillium species: how many of them are there? Mol Plant Pathol 4:297–304
Bhat RG, Subbarao KV (1999a) Green manure. In: The encyclopedia of plant pathology, vol 1, Wiley, New York, pp 519–520
Bhat RG, Subbarao KV (1999b) Host range specificity in Verticillium dahliae. Phytopathology 89:1218–1225
Bhat RG, Subbarao KV (2002) Reaction of broccoli to Verticillium dahliae isolates from various hosts. Plant Dis 85:141–146
Bjarnholt N, Laegdsmand M, Hansen HCB, Jacobsen OH, Moller BL (2008) Leaching of cyanogenic glucosides and cyanide from white clover green manure. Chemosphere 72:897–904
Bletsos F, Thanassoulopoulos C, Roupakias D (2003) Effect of grafting on growth, yield, and Verticillium wilt of eggplant. HortScience 38(2):183–186
Blok WJ, Lamers JG, Termorshuizen AJ, Bollen GJ (2000) Control of soilborne plant pathogens by incorporating fresh organic amendments followed by tarping. Phytopathology 90:253–259
Browning M, Wallace DB, Dawson C, Alm SR, Amador JA (2006) Potential of butyric acid for control of soil-borne fungal pathogens and nematodes affecting strawberries. Soil Biol Biochem 38:401–404
Butler MJ, Day AW (1998) Destruction of fungal melanins by ligninases of Phanerochaete chrysosporium and other white rot fungi. International. J Plant Sci 159:989–995
Catara V, Bella P, Polizzi G, Paratore A (2001) First report of bacterial stem rot caused by Pectobacterium carotovorum subsp. carotovorum and P. carotovorum subsp. atrosepticum on grafted eggplant in Italy. Plant Dis 85:921
Ciccarese F, Frisullo S, Cirulli M (1987) Severe outbreaks of Verticillium wilt on Cichorium intybus and Brassica rapa andpathogenic variations among isolates of Verticillium dahliae. Plant Dis 71:1144–1145
Cohen MF, Yamasaki H, Mazzola M (2005) Brassica napus seed meal soil amendment modifies microbial community structure, nitric oxide production and incidence of Rhizoctonia root rot. Soil Biol Biochem 37:1215–1227
Collins A, Okoli CAN, Morton A, Parry D, Edwards SG, Barbara DJ (2003) Isolates of Verticillium dahliae pathogenic to crucifers are of at least three distinct molecular types. Phytopathology 93:364–376
Conn KL, Lazarovits G (1999) Impact of animal manures on Verticillium wilt, potato scab, and soil microbial populations. Can J Plant Pathol 21:81–92
Conn KL, Lazarovits G (2000) Soil factors influencing the efficacy of liquid swine manure added to soil to kill Verticillium dahliae. Can J Plant Pathol 22:400–406
Conn KL, Lazarovits G (2007) Reduction of potato scab with acidified liquid swine manure soil amendment. Can J Plant Pathol 29:440
Conn KL, Tenuta M, Lazarovits G (2005) Liquid swine manure can kill Verticillium dahliae microsclerotia in soil by volatile fatty acid, nitrous acid, and ammonia toxicity. Phytopathology 95:28–35
Conn KL, Topp E, Lazarovits G (2007) Factors influencing the concentration of volatile fatty acids, ammonia, and other nutrients in stored liquid pig manure. J Environ Qual 36:440–447
Davis JR, Huisman OC, Westermann DT, Hafez SL, Everson DO, Sorensen LH, Schneider AT (1996) Effects of green manures on Verticillium wilt of potato. Phytopathology 86:44–453
Diánez F, Díaz M, Santos M, Huitrón V, Ricárdez M, Camacho F (2007) The use of grafting in Spain. Proceedings of technical meeting on non-chemical alternatives for soil-borne pest control, Budapest, pp 87–97
Durner J, Wendehenne D, Klessig DF (1998) Defense gene induction in tobacco by nitric oxide, cyclic GMP, and cyclic ADP-ribose. Proc Natl Acad Sci 95:10328–10333
Edelstein M (2004) Grafting vegetable-crop plants: pros and cons. Acta Horticulturae 659:235–238
El-Tarabily KA, Nassar AH, Hardy GE St. J, Sivasithamparam K (2003) Fish emulsion as a food base for rhizobacteria promoting growth of radish (Raphanus sativus L. var. sativus) in a sandy soil. Plant Soil 252:397–411
Gamliel A, Stapleton JJ (1993a) Effect of chicken compost or ammonium phosphate and solarization on pathogen control, rhizosphere microorganisms, and lettuce growth. Plant Dis 77:886–891
Gamliel A, Stapleton JJ (1993b) Characterization of antifungal volatile compounds evolved from solarized soil amended with cabbage residues. Phytopathology 83:899–905
Garibaldi A, Minuto A (2003) The application of grafting against soilborne pests and diseases of tomato in Italy: present situation and perspectives. Proceedings of international congress “Greenhouse tomato – Integrated crop production – Organic production”, Avignon, France, pp 55–59
Garibaldi A, Minuto A, Gullino ML (2005) Verticillium wilt incited by Verticillium dahliae in eggplant grafted on Solanum torvum in Italy. Plant Dis 89:777
Ginoux G, Laterrot H (1991) Greffage de l’aubergine: réflexion sur le choix du porte-greffe P.H.M. Revue Horticole 321:49–54
Gousset C, Collonnier C, Mulya K, Mariska I, Rotino GL, Besse P, Servaes A, Sihachakr D (2005) Solanum torvum, as a useful source of resistance against bacterial and fungal diseases for improvement of eggplant (S. melongena L.). Plant Sci 168:319–327
Hao JJ, Subbarao KV, Koike ST (2003) Effects of broccoli rotation on lettuce drop caused by Sclerotinia minor and on the sclerotial population in soil. Plant Dis 87:159–166
Haramoto ER, Gallandt ER (2004) Brassica cover cropping for weed management: A review. Renew Agric Food Sys 19:187–198
Hoes JA (1971) Development of chlamydospores in Verticillium nigriscens and V. nubilum. Can J Bot 49:1863–1866
Isaac I, MacGarvie QD (1966) Dormancy and germination of resting structures of Verticillium spp. Trans Br Mycol Soc 49:669–678
Karapapa VK, Bainbridge BW, Heale JB (1997) Morphological and molecular characterisation of Verticillium longisporum comb. nov., pathogenic to oil seed rape. Mycol Res 101:1281–1297
Khahm EM (2005) Effect of grafting on growth, performance and yield of aubergine (Solanum melongena L) in the field and greenhouse. J Food Agric Environ 3:92–94
Koike ST, Subbarao KV, Gordon TR, Davis RM, Hubbard JC (1994) Verticillium wilt of cauliflower in California. Plant Dis 78:1116–1121
Krikun J, Bernier CC (1990) Morphology of microsclerotia of Verticillium dahliae in roots of gramineous plants. Can J Plant Pathol 12:439–441
Kurata K (1994) Cultivation of grafted vegetables II. Development of grafting robots in Japan. HortScience 29:240–244
Lazarovits G (2001) Management of soilborne plant pathogens with organic soil amendments: a disease control strategy salvaged from the past. Can J Plant Pathol 23:1–7
Lazarovits G (2004) Managing soilborne plant diseases through selective soil disinfestation by a knowledge based application of soil amendments. Phytoparasitica 32:427–431
Lazarovits G, Tenuta M, Conn KL (2001) Organic amendments as a disease control strategy for soilborne diseases of high-value agricultural crops. Australas Plant Pathol 30:111–117
Lazarovits G, Conn KL, Abbasi PA, Tenuta M (2005) Understanding the mode of action of organic soil amendments provides the way for improved management of soilborne plant pathogens. Acta Horticulturae 698:215–225
Lazarovits G, Conn KL, Abbasi PA, Soltani N, Kelly W, McMillan E, Peters RD, Drake KA (2008) Reduction of potato tuber diseases with organic soil amendments in two Prince Edward Island fields. Can J Plant Pathol 30:37–45
Lee JM (1994) Cultivation of grafted vegetables. Current status, grafting methods and benefits. HortScience 29:235–239
Lewis JA, Papavizas GC (1971) Effect of sulfur-containing volatile compounds and vapors from cabbage decomposition on Aphanomyces euteiches. Phytopathology 61:208–214
Lockwood JL, Yoder OL, Bente MK (1970) Grafting eggplants on resistant rootstocks as a possible approach for control of Verticillium wilt. Plant Dis 54:846–848
Lynch VM (1977) Phytotoxicity of acetic acid produced in the anaerobic decomposition of wheat straw. J Appl Bacteriol 42:81–87
Lynch VM (1978) Production and phytotoxicity of acetic acid produced in anaerobic soils containing plant residues. Soil Biol Biochem 10:131–135
Mancini LM, Lazzeri L, Palmieri SJ (1997) In vitro fungitoxic activity of some glucosinolates and their enzyme-derived products toward plant pathogenic fungi. J Agric Food Chem 45:2768–2773
Matthiessen J, Kirkegaard J (2006) Biofumigation and enhanced biodegradation: opportunity and challenge in soilborne pest and disease management. Crit Rev Plant Sci 25:235–265
Mayton HS, Olivier C, Vaughn SF, Loria R (1996) Correlation of fungicidal activity of Brassica species with allyl isothiocyanate production in macerated leaf tissue. Phytopathology 86:267–271
Mazzola M (2004) Assessment and management of soil microbial community structure for disease suppression. Annu Rev Phytopathol 42:35–59
Mazzola M, Granatstein DM, Elfving DC, Mullinix K (2001) Suppression of specific apple root pathogens by Brassica napus seed meal amendment regardless of glucosinolate content. Phytopathology 91:673–679
Mazzola M, Brown J, Izzo AD, Cohne MF (2007) Mechanism of action and efficacy of seed meal-induce pathogen suppression differ in Brassica species and time-dependent manner. Phytopathology 97:454–460
Minuto A, Serges T, Nicotra G, Garibaldi A (2007) Applicazione dell’innesto erbaceo per le solanacee allevate in coltura protetta: problematiche e prospettive. Informatore fitopatologico – La difesa delle piante 57:30–36
Momma N (2008) Biological soil disinfestation (BSD) of soilborne pathogens and its possible mechanisms. Jpn Agric Res Quar 42:7–12
Njoroge S, Park S, Kang S, Subbarao KV (2008a) Comparative analysis of infection of broccoli and cauliflower by a GFP-tagged Verticillium dahliae isolate. (Abstr.). Phytopathology 98:S114
Njoroge SMC, Riley MB, Keinath AP (2008b) Effect of incorporation of Brassica spp. residues on population densities of soilborne microorganisms and on damping-off and Fusarium wilt of watermelon. Plant Dis 92:287–294
Nothamann J, Ben-Yephet Y (1979) Screening of eggplant and other Solanum species for resistance to Verticillium dahliae. Plant Dis Reporter 63:70–73
Oka Y, Shapira N, Fine P (2007) Control of root-knot nematodes in organic farming systems by organic amendments and soil solarization. Crop Prot 26:1556–1565
Pegg GF, Brady BL (2002) Verticillium wilts. CABI, New York, p 552
Qin Q-M, Vallad GE, Wu BM, Subbarao KV (2006) Phylogenetic analyses of phytopathogenic isolates of Verticillium. Phytopathology 96:582–592
Ramirez-Villapudua J, Munnecke DE (1988) Effect of solar heating and soil amendments of cruciferous residues on Fusarium oxysporum f. sp. conglutinans and other organisms. Phytopathology 78:289–295
Rivero RM, Ruiz JM, Romero L (2003) Role of grafting in horticultural plants under stress conditions. J Food Agric Environ 1:70–74
Scopa A, Dumontet S (2007) Soil solarization: effects on soil microbiological parameters. J Plant Nutr 30:537–547
Shetty KG, Subbarao KV (1999) Melanolytic activity of microorganisms and antagonism to Verticillium dahliae. Phytopathology 89 (Suppl.):S72
Shetty KG, Subbarao KV, Huisman OC, Hubbard JC (2000) Mechanism of broccoli-mediated Verticillium wilt reduction in cauliflower. Phytopathology 90:305–310
Subbarao KV, Hubbard JC (1996) Interactive effects of broccoli residue and temperature on Verticillium dahliae microsclerotia in soil and on wilt in cauliflower. Phytopathology 86:1303–1309
Subbarao KV, Chassot A, Gordon TR, Hubbard JC, Bonello P, Mullin R, Okamoto D, Davis RM, Koike ST (1995) Host range of Verticillium dahliae from cauliflower and genetic relationships and cross pathogenicities of isolates from different crops. Phytopathology 85:1105–1112
Subbarao KV, Hubbard JC, Koike ST (1999) Evaluation of broccoli residue incorporation into field soil for Verticillium wilt control in cauliflower. Plant Dis 83:124–129
Tenuta M, Lazarovits G (2002a) Ammonia and nitrous acid from nitrogenous amendments kill microsclerotia of Verticillium dahliae. Phytopathology 92:255–264
Tenuta M, Lazarovits G (2002b) Identification of specific soil properties that affect the accumulation and toxicity of ammonia to Verticillium dahliae. Can J Plant Pathol 24:219–229
Tenuta M, Lazarovits G (2004) Soil properties associated with the variable effectiveness of meat and bone meal to kill microsclerotia of Verticillium dahliae. Appl Soil Ecol 25:219–236
Tenuta M, Conn KL, Lazarovits G (2002) Volatile fatty acids in liquid swine manure can kill microsclerotia of Verticillium dahliae. Phytopathology 92:548–452
Thorup-Kristensen K, Magid J, Jensen LS (2003) Catch crops and green manures as biological tools in nitrogen management in temperate zones. Adv Agron 51:227–302
Traka-Mavrona E, Koutsika-Sotiriou M, Pritsa T (2000) Response of squash (Cucurbita spp.) as rootstock for melon (Cucumis melo L.). Sci Hortic 83:353–362
Trankner A (1992) Use of agricultural and municipal organic wastes to develop suppressiveness to plant pathogens. In: Tjamos ES, Papavizas GC, Cook RJ (eds) Biological control of plant diseases. Plenum, New York, pp 35–42
Tsao PH, Oster JJ (1981) Relation of ammonia and nitrous acid to suppression of Phytophthora in soils amended with nitrogenous organic substances. Phytopathology 71:53–59
Vallad GE, Bhat RG, Koike ST, Subbarao KV, Ryder EJ (2005a) Weedborne reservoirs and seedborne transmission of Verticillium dahliae in lettuce. Plant Dis 89:317–324
Vallad GE, Qin QM, Subbarao KV (2005b) Verticillium wilt of cool season vegetable crops: their distribution, impact and management. In: Recent research developments in plant pathology. Research Signpost, Trivandrum, India, pp 189–210
Warren KS (1962) Ammonia toxicity and pH. Nature 195:47–49
Wilhelm S (1951) Effect of various soil amendments on the inoculum potential of the Verticillium wilt fungus. Phytopathology 41:684–690
Xiao CL, Subbarao KV, Schulbach KF, Koike ST (1998) Effects of crop rotation and irrigation on Verticillium dahliae microsclerotia in soil and wilt in cauliflower. Phytopathology 88:1046–1055
Xiao J, Zhu J, Chen S, Ruan W, Miller C (2007) A novel use of anaerobically digested liquid swine manure to potentially control soybean cyst nematode. J Environ Sci Health, Part B 42:749–757
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Lazarovits, G., Subbarao, K. (2010). Challenges in Controlling Verticillium Wilt by the Use of Nonchemical Methods. In: Gisi, U., Chet, I., Gullino, M. (eds) Recent Developments in Management of Plant Diseases. Plant Pathology in the 21st Century, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8804-9_18
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