Abstract
Sol-gel method was used to synthesize polysiloxane hydrogel with encapsulated urease (immobilization degree in the range of 79–88%) which preserved the enzymatic activity at the level of 56–84%. The nature of functional groups was shown to influence the pore structure parameters. Immobilization degree and preservation of adsorbed urease activity depend on the structural-adsorption characteristics of matrices. The possibility of “double immobilization” of urease on silica gel by sol-gel method and the opportunity of reuse of the synthesized formulations was investigated. Urease immobilization on the surface of magnetite (FeO·Fe2O3) was also studied by adsorption method.
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Pogorilyi, R.P., Honcharyk, V.P., Melnyk, I.V., Zub, Y.L. (2008). Application of Sol-Gel Method for Synthesis of a Biosensitive Polysiloxane Matrix. In: Innocenzi, P., Zub, Y.L., Kessler, V.G. (eds) Sol-Gel Methods for Materials Processing. NATO Science for Peace and Security Series C: Environmental Security. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8514-7_32
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DOI: https://doi.org/10.1007/978-1-4020-8514-7_32
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