Abstract
The pinewood nematode Bursaphelenchus xylophilus is the causal agent of pine wilt disease. In order to trace the origin of its recently introduced Portuguese population, two PCR-based techniques, random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR), were used to determine genetic relationships among 30 B. xylophilus isolates from the USA, Canada, Japan, China, South Korea and Portugal. Fingerprints obtained with both methods detected a reduced genetic variation of introduced isolates as compared to native North American isolates. Cluster analyses of genetic distances between isolates were carried out and bootstrap dendrograms were constructed. The results indicated that founders of the Portuguese isolates most likely were translocated one or two times to Portugal from their colonized sites in East Asia, but not from their native habitats in North America.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Evans, H.F., McNamara, D.G., Braasch, H., Chadoeuf, J. and Magnusson, c. (1996). Pest Risk Analysis (PRA) for the territories of the European Union (as PRA area) on Bursaphelenchus xylophilus and its vectors in the genus Monochamus. EPPO Bulletin 26: 99–249.
Hood, G.M. (2005). PopTools version 2.6.6. www.cse.csiro.au/poptools.
Mantel, N. (1967). The detection of disease clustering and generalized regression approach. Cancer Research 27: 209–220.
Metge, K. and Burgermeister, W. (2005). Molecular identification and pathway analysis of the introduced pinewood nematode Bursaphelenchus xylophilus. In Plant Protection and Plant Health in Europe: Introduction and Spread of Invasive Species. BCPC Symposium Proceedings (eds. D.V. Alford and G.F. Backhaus), pp. 259–260.
Metge, K. and Burgermeister, W. (2006) Intraspecific variation of isolates of Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae) revealed by ISSR and RAPD fingerprints. Journal of Plant Disease and Protection 6: 275–282.
Metge, K., Schröder, T. and Burgermeister, W. (2004). Biogeographische Untersuchungen zur Herkunft des nach Europa eingeschleppten Kiefernholznematoden Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae). Mitt. Biol. Bundesanst. 396: 296–297.
Mota, M., Braasch, H., Bravo, M.A., Penas, A.C., Burgermeister, W., Metge, K. and Sousa, E. (1999). First report of Bursaphelenchus xylophilus in Portugal and in Europe. Nematology 1: 727–734.
Nei, M. and Li, W.H. (1979). Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Nat. Acad. Sci. USA 76: 5269–5273.
Pfeilstetter, E. (2003). Monitoring invasiver Arten vor dem Hintergrund pflanzengesundheitlicher Regelungen [Monitoring of invasive species in relation to phytosanitary regulations]. Schriftenr. BMVEL A Angew. Wiss. 498: 233–237.
Saitou, N. and Nei, M. (1987). The Neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406–425.
Sakai, A.K., Allendorf, F.W., Holt, J.S., Lodge, D.M., Molofsky, J., With, K.A., Baughman, S., Cabin, R.J., Cohen, J.E., Ellstrand, N.C., McCauley, D.E., O’Neil, P., Parker, J.M., Thompson, J.N. and Weller, S.G. (2001). The population biology of invasive species. Annu. Rev. Ecol. Syst. 32: 305–332.
Schmitz, B., Burgermeister, W. and Braasch, H. (1998). Molecular genetic classification of Central European Meloidogyne chitwoodi and M. fallax populations. Nachrichtenbl. Deut. Pflanzenschutzd. 50: 310–317.
Sneath, P.H.A. and Sokal, R.R. (1973). Numerical Taxonomy: The principles and Practice of Numerical Classification. W. H. Freeman and Company, San Francisco, CA, USA.
Sousa, E., Bravo, M.A., Pires, J., Naves, P., Penas, A.C., Bonifacio, L. and Mota, M. (2001). Bursaphelenchus xylophilus (Nematoda; Aphelenchoididae) associated with Monochamus galloprovincialis (Coleoptera; Cerambycidae) in Portugal. Nematology 3: 89–91.
Swofford, D.L. (1998). Phylogenetic Analysis Using Parsimony (* and other methods). Version 4. Sinauer Associates, Sunderland, MA, USA
Tomiczek, C., Braasch, H., Burgermeister, W., Metge, K. Hoyer, U. and Bandstetter, M. (2003). Identification of Bursaphelenchus spp. isolated from Chinese packaging wood imported to Austria. Nematology 5: 573–581.
Vieira, P., W. Burgermeister, M. Mota, K. Metge & G. Silva (2007). Lack of genetic variation of Bursaphelenchus xylophilus in Portugal revealed by RAPD-PCR analyses. Journal of Nematology 39(2): 118–126.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2008 Springer Science+Business Media B.V.
About this chapter
Cite this chapter
Metge, K., Burgermeister, W. (2008). Analysis of Bursaphelenchus xylophilus (Nematoda: Parasitaphelenchidae) Provenances Using ISSR and RAPD Fingerprints. In: Mota, M.M., Vieira, P. (eds) Pine Wilt Disease: A Worldwide Threat to Forest Ecosystems. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8455-3_15
Download citation
DOI: https://doi.org/10.1007/978-1-4020-8455-3_15
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-8454-6
Online ISBN: 978-1-4020-8455-3
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)