Abstract
Internal transcribed spacers and 28S D2/D3 domain of rDNA were used to infer the phylogenetic relationships among Bursaphelenchus species, with special emphasis on members of the xylophilus and fungivorus groups. Sequence alignments and phylogenetic analysis using neighbour-joining and maximum parsimony algorithms resulted in trees with similar topologies. The 17 Bursaphelenchus species examined can be separated into two main branches: the first includes members of the xylophilus group and the second includes the species of the fungivorus group, separated from the remaining species B. eremus, B. hofmanni, B. rainulfi and B. yongensis. As far as known, the species of the xylophilus group are phoretically associated with longhorn beetles. The phylogenetic analysis revealed the digger bee associated B. abruptus as the basal taxon of the species investigated or at least of the xylophilus group. The remainder of the species are, as far as known, associated with bark beetles and a soil-dwelling bee in case of B. seani. The significantly supported groups are largely
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Anthoine, G. and Mugniery, D. (2005). Variability of the ITS rDNA and identification of Nacobbus aberrans (Thorne, 1935) Thorne and Allen, 1944 (Nematoda: Pratylenchidae) by rDNA amplification. Nematology 7: 503–516.
Beckenbach, K., Blaxter, M. and Webster, J.M. (1999). Phylogenetic of Bursaphelenchus species derived from analysis of ribosomal internal transcribed spacer DNA sequences. Nematology 1: 539–548.
Blok, V.C., Malloch, G., Harrower, B., Phillips, M.S. and Vrain T.C. (1998). Intraspecific variation in ribosomal DNA in populations of the potato cyst nematode Globodera pallida. Journal of Nematology 30: 262–273.
Boutsika, K., Brown, D.J.F., Phillips, M.S. and Blok, V.C. (2004). Molecular characterisation of the ribosomal DNA of Paratrichodorus macrostylus, P. pachydermus, Trichodorus primitivus and T. similis (Nematoda: Trichodoridae). Nematology 6:641–654.
Braasch, H. (2001). Bursaphelenchus species in conifers in Europe: distribution and morphological relationships. European and Mediteranean Plant Protection Organisation, OEPP/EPPO, Bulletin 31: 127–142.
Braasch, H., Burgermeister, W., Schröder, T., Apel, K.-H. and Brandstetter, M. (2006). Occurrence of Bursaphelenchus eremus Rühm, 1956 (Nematoda: Parasitaphelenchidae) on oaks in State Brandenburg/Germany and supplementary species description. Nachrichtenblatt für den Deutschen Pflanzenschutzdienst 58: 148–153.
Braasch, H., Metge, K. and Burgermeister, W. (1999). Bursaphelenchus-Arten (Nematoda, Parasitaphelenchidae) in Nadelgehölzen in Deutschland und ihre ITS-RFLP-Muster. Nachrichtenblatt Deutscher Pflanzenschutzdienst 51: 312–320.
Burgermeister, W., Metge, K., Braasch, H. and Buchbach, E. (2005). ITS-RFLP patterns for differentiation of 26 Bursaphelenchus species (Nematoda: Parasitaphelenchidae) and observations on their distribution. Russian Journal of Nematology 13: 29–42.
Giblin-Davis, R.M., Hazir, S., Center, B.J., Ye, W., Keskin, N., Thorp, R.W. and Thomas, K. (2005). Bursaphelenchus anatolius n. sp. (Nematoda: Parasitaphelenchidae), an associate of bees in the genus Halictus. Journal of Nematology 37: 336–342.
Giblin-Davis, R.M., Mundo-Ocampo, M., Baldwin, J.G., Norden, B.B. and Batra, S.W.T. (1993). Description of Bursaphelenchus abruptus n. sp. (Nemata: Aphelenchoididae), an associate of a digger bee. Journal of Nematology 25: 161–172.
Giblin, R.M. and Kaya, H.K. (1983). Bursaphelenchus seani n. sp. (Nematoda, Aphelenchoididae), a phoretic associate of Anthophora bomboides stanfordiana Cockerell, 1904 (Hymenoptera, Anthophoridae). Revue de Nématologie 6: 39–50.
Gu, J., Braasch, H., Burgermeister, W., Brandstetter, M. and Zhang, J. (2006). Description of Bursaphelenchus yongensis sp.n. (Nematoda: Parasitaphelenchidae) isolated from Pinus massoniana in China. Russian Journal of Nematology 14: 91–99.
Hall, T.A. (1999). BioEdit: a user-friendly biological sequences alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium 41: 95–98.
Iwahori, H., Tsuda, K., Kanzaki, N., Izui, K. and Futai, K. (1998). PCR-RFLP and sequencing analysis of ribosomal DNA of Bursaphelenchus nematodes related to pine wilt disease. Fundamental and Applied Nematology 21: 655–666.
Kang, J.S., Choi, K.S., Shin, S.C., Moon, I.S., Lee, S.G. and Lee, S.H. (2004). Development of an efficient PCR-based diagnosis protocol for the identification of the pinewood nematode, Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae). Nematology6: 279–285.
Kanzaki, N. and Futai, K. (2002). A PCR primer set for determination of phylogenetic relationships of Bursaphelenchus species within the xylophilus group. Nematology 4: 35–41.
Kumar, S., Tamura, K. and Nei, M. (2004). MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Briefings in Bioinformatics 5: 150–163.
Matsunaga, K. and Togashi, K. (2004). A simple method for discriminating Bursaphelenchus xylophilus and B. mucronatus by species-spezific polymerase chain reaction primer pairs. Nematology 6: 273–277.
Metge, K., Braasch, H., Gu, J. and Burgermeister, W. (2006). Interspecific variation in ITS rDNA of Bursaphelenchus species (Nematoda: Parasitaphelencidae) of different groups. Russian Journal of Nematology 14: 147–158.
Metge, K. and Burgermeister, W. (2005). Multiple displacement amplification of DNA for ITS-RFLP analysis of Bursaphelenchus individual nematodes. Nematology7: 253–257.
Mota, M.M., Braasch, H., Bravo, M.A., Penas, A.C., Burgermeister, W., Metge, K. and Sousa, E. (1999). First report of Bursaphelenchus xylophilus in Portugal and in Europe. Nematology 1: 727–734.
Powers, T.O., Todd, T.C., Burnell, A.M., Murray, P.C.B., Fleming, C.C., Szalanski, A.L., Adams, B.A. and Harris, T.S. (1997). The rDNA internal transcribed spacer region as a taxonomic marker for nematodes. Journal of Nematology 29: 441–450.
Ryss, A., Vieira, P., Mota, M. and Kulinich, O. (2005). A synopsis of the genus Bursaphelenchus Fuchs, 1937 (Aphelenchida: Parasitaphelenchidae) with keys to species. Nematology 7: 393–458.
Schönfeld, U., Braasch, H. and Burgermeister, W. (2006). Bursaphelenchus spp. (Nematoda: Parasitaphelenchidae) in wood chips from sawmills in Brandenburg and description of Bursaphelenchus willibaldi sp. n. Russian Journal of Nematology 14:119–126.
Subbotin, S.A., Halford, P.D., Warry, A. and Perry, R.N. (2000). Variation in ribosomal DNA sequences and phylogeny of Globodera parasitising solanaceous plants. Nematology 2: 591–604.
Zheng, J., Subbotin, S.A., He, S., Gu, J. and Moens, M. (2003). Molecular characterisation of some Asian isolates of Bursaphelenchus xylophilus and B. mucronatus using PCR-RFLPs and sequences of ribosomal DNA. Russian Journal of Nematology 11: 17–22.
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Metge, K., Braasch, H., Gu, J., Burgermeister, W. (2008). Variation in ITS and 28S rDNA of Bursaphelenchus Species (Nematoda: Parasitaphelenchidae). In: Mota, M.M., Vieira, P. (eds) Pine Wilt Disease: A Worldwide Threat to Forest Ecosystems. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8455-3_13
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