Abstract
The possibility of the recovery of endangered sturgeon species is considered, with special reference to the production of androgenetic nucleocytoplasmic hybrids by dispermic androgenesis. The method of dispermic androgenesis, developed for sturgeon fishes, includes genetic inactivation of eggs, their insemination with concentrated sperm (to cause polyspermy), and heat shock to facilitate the fusion of male pronuclei. The restoration of the diploid state of androgenotes by fusion of two sperm nuclei allows androgenetic progeny to have a heterozygosity level similar to that in a regular crossing. Using this method, viable androgenetic progenies of several sturgeon species were obtained for the first time. Here we present the results of the first successful experiments in obtaining androgenetic hybrids in sturgeon fishes by dispermic androgenesis. Both the androgenetic hybrids, between stellate and great sturgeons and between Persian and Russian sturgeons, obtained in these experiments were fully viable. It was shown that androgenetic hybrids had the nuclear DNA of the paternal species and the mitochondrial DNA of the maternal species. The morphological analysis proved the androgenetic hybrids between Persian and Russian sturgeons to be completely identical to the paternal species at the age of 12 months, and those between stellate and great sturgeons by 3 years. The prolonged manifestation of matrocliny observed in both hybrids is most likely related to the extended life cycle of acipenserids. Our results suggest that endangered sturgeon fishes may be recovered by means of dispermic androgenesis if the sperm alone of a given species is available.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Astaurov BL, Ostryakova-Varshaver VP. 1957. Production of full heterospermic androgenesis and interspecific hybrids in silkworm: experimental analysis of relative roles of nucleus and cytoplasm in development and heredity. Izv Akad Nauk SSSR. Ser Biol 2:154–175.
Birstein VJ, Hanner R, DeSalle R. 1997a. Phylogeny of the Acipenseriformes: cytogenetic and molecular approaches. Environ Biol Fish 48:127–155.
Birstein VJ, Bemis WE, Waldman JR. 1997b. The threatened status of acipenseriform fishes: a summary. Environ Biol Fish 48:427–444.
Chao N-H, Liao ICh. 2001. Cryopreservation of finfish and shellfish gametes and embryos. Aquaculture 197:161–189.
Corley-Smith GE, Brandhorst BP. 1999. Preservation of endangered species and populations: a role for genome banking, somatic cell cloning, and androgenesis? Mol Reprod Devel 53:363–367.
DeSalle R, Birstein VJ. 1997. Method and compositions for identification of species origin of caviar. Appl. No. 97107809.2. European Patent Application Bulletin, p. 47.
Dettlaff TA, Ginsburg AS, Schmalhausen OI. 1993. Sturgeon Fishes. Developmental Biology and Aquaculture. Springer-Verlag, Berlin, 300 pp.
Devlin RH, Nagahama Y. 2002. Sex determination and sex differentiation in fish: an overview of genetic, physiological, and environmental influences. Aquaculture 208:191–364.
Ginsburg AS. 1968. Oplodotvorenie u ryb i problema polispermii (Fertilization in Fish and Problem of Polyspermy). Nauka, Moscow, 358 pp (in Russian).
Grunina AS, Neyfakh AA. 1991. Induction of diploid androgenesis in Siberian sturgeon Acipenser baerii Brandt. Ontogenez 22:53–56 (in Russian with English summary).
Grunina AS, Neyfakh AA. 1997. Induced diploid androgenesis. Physiol Gen Biol Rev 12:73–103.
Grunina AS, Recoubratsky AV. 2005. Induced androgenesis in fish: obtaining viable nucleocytoplasmic hybrids. Russ J Dev Biol 36:208–217.
Grunina AS, Recoubratsky AV, Neyfakh AA. 1995. Induced diploid androgenesis in sturgeons. Sturgeon Quart 3:6–7.
Holčik J, Bǎnǎrescu P, Evans D. 1989. General introduction to fishes. In: Holčik J (ed.), The Freshwater Fishes of Europe. 1. Pt. 2. AULA-Verlag, Wiesbaden, pp. 18–147.
Lubzens E, Pekarsky I, Magnus Y, Ar A. 1996. Term and prospect for long term storage of teleost ova and embryos. In: Proceedings of the Conference of Refrigeration and Aquaculture, Bordeaux, pp. 491–501.
Mims SD, Shelton WL, Linhart O, Wang C. 1997. Induced meiotic gynogenesis of paddlefish Polyodon spathula. J World Aquacult Soc 28:334–343.
Neyfakh AA. 1999. Nucleo-cytoplasmic incompatibility of androgenetic hybrids in sturgeons. J Appl Ichthyol 15:318–319.
Neyfakh AA, Radzievskaya VV. 1967. On morphogenetic nuclear function in standard and androgenetic goldfish/loach hybrids (Carassius auratus × Misgurnus fossilis). Genetika 12:80–88 (in Russian with English summary).
Penman DJ, Myers JM, McAndrew BJ. 1996. Restoration of diploid genotypes by androgenesis. In: Proceedings of the Conference Refrigeration and Aquaculture, Bordeaux, pp. 469–474.
Recoubratsky AV, Grunina AS, Minin AA, Duma LN, Neyfakh AA. 1996. Dispermic androgenesis in Acipenser stellatus. Sturgeon Quart 4:12–14.
Recoubratsky AV, Grunina AS, Myuge NS, Neyfakh AA. 1998. Production of androgenetic nucleocytoplasmic hybrids in sturgeon fish. Russ J Dev Biol 29:224–229.
Recoubratsky AV, Grunina AS, Barmintsev VA, Golovanova TS, Chudinov OS, Abramova AB, Panchenco NS, Kupchenko SA. 2003. Meiotic gynogenesis in Stellate and Russian sturgeons and Sterlet. Russ J Dev Biol 34:92–101 (in Russian).
Suquet M, Dreanno C, Fauvel C, Cosson J, Billard R. 2000. Cryopreservation of sperm in marine fish. Aquacult Res 31:231–243.
Van Eenennaam AL, Van Eenennaam JP, Medrano JF, Doroshov SI. 1999. Evidence of female hetero-gametic genetic sex determination in white sturgeon. J Hered 90:231–233.
Vasil’ev VP. 1985. Evolutionary Karyology of Fishes. Nauka, Moscow, 300 pp (in Russian).
Vasil’eva ED. 1999. Some morphological characteristics of Acipenserid fishes: considerations of their variability and utility in taxonomy. J Appl Ichthyol 15:32–34.
Vasil’eva ED. 2004. Morphological data corroborating the assumption of independent origins within octoploid sturgeon species. J Ichthyol 44(Suppl. 1):63–72.
Vasil’eva ED, Grunina AS, Recoubratsky AV. 2001. The pattern of manifestation of some morphological characters in androgenetic nucleo-cytoplasmatic hybrids of the Persian Acipenser persicus and Russian A. gueldenstaedtii sturgeons in the postlarval period. J Ichthyol 41:454–460.
Vasil’eva ED, Grunina AS, Recoubratsky AV, Pavlinov IYa. 2005. Manifestation pattern of some morphological characters in androgenetic nucleocytoplasmatic hybrids between stellate sturgeon Acipenser stellatus and great sturgeon Huso huso (Acipenseridae) during postlarval ontogeny. J Ichthyol 45:465–478.
Veprintsev BN, Rott NN. 1979. Conserving genetic resources of animal species. Nature 280:633–634.
Verigin BV, Makeeva AP. 1972. Hybridization of Carp with Aristichthys nobilis. Genetika 8(7): 55–64 (in Russian).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Springer Science + Business Media B.V
About this chapter
Cite this chapter
Grunina, A.S., Recoubratsky, A.V., Barmintsev, V.A., Vasil’eva, E.D., Chebanov, M.S. (2009). Dispermic Androgenesis as a Method for Recovery of Endangered Sturgeon Species. In: Carmona, R., Domezain, A., García-Gallego, M., Hernando, J.A., Rodríguez, F., Ruiz-Rejón, M. (eds) Biology, Conservation and Sustainable Development of Sturgeons. Fish & Fisheries Series, vol 29. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8437-9_11
Download citation
DOI: https://doi.org/10.1007/978-1-4020-8437-9_11
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-8436-2
Online ISBN: 978-1-4020-8437-9
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)