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The role of rhizosphere microorganisms in relation to P uptake by plants

  • Petra Marschner
Chapter
Part of the Plant Ecophysiology book series (KLEC, volume 7)

The rhizosphere is defined as the soil around the roots that is influenced by the root (Hiltner 1904). Due to the release of easily decomposable compounds by the roots (root exudates), the rhizosphere is characterized by high microbial density. Rhizosphere microorganisms strongly influence nutrient uptake by plants by either enhancing or decreasing nutrient availability.

Rhizosphere microbial communities are a subset of the soil microbial community, but are often quite distinct from those in the bulk soil (Foster 1986; Marilley and Aragno 1999; Gomes et al. 2001; Berg et al. 2002). Rhizosphere communities are influenced by soil and plant factors. Soils can have distinct microbial communities (Gelsomino et al. 1999; Carelli et al. 2000), as a result of the soil physical and chemical characteristics (e.g. soil texture, nutrient and organic matter content and pH) and environmental factors such as climate and vegetation. Plants contribute to these physical and chemical properties by depositing between 1% and 25% of their net photosynthetic production, which includes dead roots, sloughed-off cells and soluble compounds (Merbach et al. 1999). A large proportion of the root exudates such as sugars, organic acid anions or amino acids are easily degradable by microorganisms in the rhizosphere resulting in high microbial density and activity in the rhizosphere (Foster 1986; Kandeler et al. 2001).

Keywords

Microbial Biomass Plant Soil Soil Biol Appl Environ Rhizosphere Microorganism 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Antoun H, Beauchamp CJ, Goussard N, Chabot R, Lalande R (1998) Potential of Rhizobium and Bradyrhizobium species as plant growth promoting rhizobacteria on non-legumes: effect on radishes (Raphanus sativus L.). Plant Soil 204: 57–67CrossRefGoogle Scholar
  2. Azcon-Aguilar C, Gianinazzi-Pearson V, Fardeau JC, Gianinazzi S (1986) Effect of vesicular-arbuscular mycorrhizal fungi and phosphate-solubilizing bacteria on growth and nutrition of soybean in a neutral-calcareous soil amended with 32P-45Ca-tricalcium phosphate. Plant Soil 96: 3–15CrossRefGoogle Scholar
  3. Badr el-Din SMS, Khalafallah MA, Moawad H (1986) Response of soybean to dual inoculation with Rhizobium japonicum and phosphate dissolving bacteria. Z Pflanz Bodenkunde 149: 130–135CrossRefGoogle Scholar
  4. Banik S, Dey BK (1983) Phosphate-solubilizing potentiality of the microorganisms capable of utilizing al phosphate as a sole phosphorus source. Zbl Mikrobiol 138: 17–23Google Scholar
  5. Barthakur HP (1978) Solubilization of relatively insoluble phosphate by some fungi isolated from the rhizosphere of rice. Indian J Agric Sci 48: 762–766Google Scholar
  6. Berg G, Roskot N, Steidle A, Eberl L, Zock A, Smalla K (2002) Plant-dependent genotypic and phenotypic diversity of antagonistic rhizobacteria isolated from different Verticillium host plants. Appl Environ Microbiol 68: 3328–3338PubMedCrossRefGoogle Scholar
  7. Carelli M, Gnocchi S, Fancelli S, Mengoni A, Paffetti D, Scotti C, Bazzicalupo M (2000) Genetic diversity and dynamics of Sinorhizobium meliloti populations nodulating different alfalfa cultivars in Italian soils. Appl Environ Microbiol 66: 4785–4789PubMedCrossRefGoogle Scholar
  8. Colpaert JV, Van Laere A, Van Tichelen KK, Van Assche JA (1997) The use of inositol hexaphosphate as a phosphorus source by mycorrhizal and non-mycorrhizal Scots pine (Pinus sylvestris). Funct Ecol 11: 407–415CrossRefGoogle Scholar
  9. De Leij FAAM, Whipps JM, Lynch JM (1993) The use of colony development for the characterization of bacterial communities in soil and on roots. Microbiol Ecol 27: 81–97Google Scholar
  10. Delvasto P, Valverde A, Ballester A, Igual JM, Munoz JA, Gonzalez F, Blazques ML, Garcia C (2006) Characterization of brushite as a re-crystallization product formed during bacterial solubilization of hydroxyapatite in batch cultures. Soil Biol Biochem 38: 2645–2654CrossRefGoogle Scholar
  11. Deubel A, Gransee A, Merbach W (2000) Transformation of organic rhizosdeposits by rhizosphere bacteria and its influence on the availability of tertiary calcium phosphate. J Plant Nutr Soil Sci 163: 387–392CrossRefGoogle Scholar
  12. Foster RC (1986) The ultrastructure of the rhizoplane and rhizosphere. Ann Rev Phytopathol 24: 211–234CrossRefGoogle Scholar
  13. Frankenberger WT, Poth M (1987) Biosynthesis of indole-3-acetic acid by the pine ectomycorrhizal fungus Pisolithus tinctorius. Appl Environ Microbiol 53: 2908–2913PubMedGoogle Scholar
  14. Frey-Klett P, Pierrat JC, Garbaye J (1997) Location and survival of mycorrhiza helper Pseudomonas fluorescens during establishment of ectomycorrhizal symbiosis between Laccaria bicolor and Douglas fir. Appl Environ Microbiol 63: 139–144PubMedGoogle Scholar
  15. Garbaye J (1994) Helper bacteria: a new dimension to the mycorrhizal symbiosis. New Phytol 128: 197–210CrossRefGoogle Scholar
  16. Gelsomino A, Keijzer WAC, Cacco G, Van Elsas JD (1999) Assessment of bacterial community structure in soil by polymerase chain reaction and denaturing gradient gel electrophoresis. J Microbiol Meth 38: 1–15CrossRefGoogle Scholar
  17. George TS, Richardson AE, Simpson RJ (2005) Behaviour of plant-derived extracellular phytase upon addition to soil. Soil Biol Biochem 37: 977–988CrossRefGoogle Scholar
  18. George TS, Simpson RJ, Gregory PJ, Richardson AE (2007) Differential interaction of Aspergillus niger and Peniophora lycii phytases with soil particles affects the hydrolysis of inositol phosphates. Soil Biol Biochem 39: 793–803CrossRefGoogle Scholar
  19. Gerretsen FC (1948) The influence of microorganisms on the phosphate intake by the plant. Plant Soil 1: 51–81CrossRefGoogle Scholar
  20. Geurts R, Franssen H (1996) Signal transduction in Rhizobium-induced nodule formation. Plant Physiol 112: 447–453PubMedCrossRefGoogle Scholar
  21. Gomes NCM, Heuer H, Schoenfeld J, Costa R, Mendoca-Hagler L, Smalla K (2001) Bacterial diversity of the rhizosphere of maize (Zea mays) grown in tropical soil studied by temperature gradient gel electrophoresis. Plant Soil 232: 167–180CrossRefGoogle Scholar
  22. Gomes NCM, Fagbola O, Costa R, Rumjanek NG, Buchner A, Mendona-Hagler L, Smalla K (2003) Dynamics of fungal communities in bulk and maize rhizosphere soil in the Tropics. Appl Environ Microbiol 69: 3758–3766PubMedCrossRefGoogle Scholar
  23. Grayston SJ, Wang S, Campbell CD, Edwards AC (1998) Selective influence of plant species on microbial diversity in the rhizosphere. Soil Biol Biochem 30: 369–378CrossRefGoogle Scholar
  24. Hayes JE, Simpson RJ, Richardson AE (2000) The growth and phosphorus utilisation of plants in sterile media when supplied with inositol hexaphosphate, glucose-1-phosphate of inorganic phosphate. Plant Soil 220: 165–174CrossRefGoogle Scholar
  25. He ZL, O’Donnell AG, Syers JK (1997) Seasonal responses in microbial biomass carbon, phosphorus and sulphur in soils under pasture. Biol Fert Soils 24: 421–428CrossRefGoogle Scholar
  26. Hiltner L (1904) Über neuere Erfahrungen und Probleme der Bodenbakteriologie unter besonderer Berücksichtigung der Gründüngung und Brache. Arb Deut Landwirt Ges 98: 59–78Google Scholar
  27. Hoberg E, Marschner P, Lieberei R (2005) Organic acid exudation and pH changes by Gordonia sp. and Pseudomonas fluorescens grown with P adsorbed to goethite. Microbiol Res 160: 177–187PubMedCrossRefGoogle Scholar
  28. Hoffland E, Findenegg GR, Nelemans JA (1989) Solubilization of rock phosphate by rape. II Local root exudation of organic acids as a response to P-starvation. Plant Soil 113: 161–165CrossRefGoogle Scholar
  29. Ibekwe AM, Kennedy AC (1998) Fatty acid methyl ester (FAME) profiles as a tool to investigate community structure if two agricultural soils. Plant Soil 206: 151–161CrossRefGoogle Scholar
  30. Illmer P, Schinner F (1995) Solubilization of inorganic calcium phosphates - solubilization mechanisms. Soil Biol Biochem 27: 257–263CrossRefGoogle Scholar
  31. Illmer P, Barbato A, Schinner F (1995) Solubilization of hardly-soluble AlPO4 with P-solubilizing microorganisms. Soil Biol Biochem 27: 265–270CrossRefGoogle Scholar
  32. Iyamuremye F, Dick RP, Baham J (1996) Organic amendments and phosphorus dynamics. I. Phosphorus chemistry and sorption. Soil Sci 161: 426–435CrossRefGoogle Scholar
  33. Joner EJ, Johansen A (2000) Phosphatase activity of external hyphae of two arbuscular mycorrhizal fungi. Mycol Res 104: 81–86CrossRefGoogle Scholar
  34. Jones DL (1998) Organic acids in the rhizosphere - a critical review. Plant Soil 205: 25–44CrossRefGoogle Scholar
  35. Kandeler E, Marschner P, Tscherko D, Gahoonia TS, Nielsen NE (2001) Microbial community composition and functional diversity in the rhizosphere of maize. Plant Soil 238: 301–312CrossRefGoogle Scholar
  36. Kim KY, Jordan D, Krishan HB (1997a) Rahella aquatilis, a bacterium isolated from soybean rhizosphere, can solubilize hydroxyapatite. FEMS Microbiol Lett 153: 273–277Google Scholar
  37. Kim KY, McDonald GA, Jordan D (1997b) Solubilization of hydroxyapatite by Enterobacter agglomerans and cloned Escherichia coli in culture medium. Biol Fert Soils 24: 347–352CrossRefGoogle Scholar
  38. Koide RT, Kabir Z (2000) Extraradical hyphae of the mycorrhizal fungus Glomus intraradices can hydrolyse organic phosphate. New Phytol 148: 511–517CrossRefGoogle Scholar
  39. Kumar V, Narula N (1999) Solubilization of inorganic phosphates and growth emergence of wheat as affected by Azotobacter chroococcum mutants. Biol Fert Soils 28: 301–305CrossRefGoogle Scholar
  40. Kundu BS, Gaur AC (1980) Establishment of nitrogen-fixing and phosphate-solubilizing bacteria in the rhizosphere and their effect on yield and nurtrient uptake of wheat crop. Plant Soil 57: 223–230CrossRefGoogle Scholar
  41. Louw HA (1969) A study of the phosphate-dissolving bacteria in the root region of wheat and lupin. Phytophylactica 2: 21–26Google Scholar
  42. Lugtenberg BJJ, Dekkers LC (1999) What makes Pseudomonas bacteria rhizosphere competent? Environ Microbiol 1: 9–13PubMedCrossRefGoogle Scholar
  43. Mansfeld-Giese K, Larsen J, Bodker L (2002) Bacterial populations associated with mycelium of the arbuscular mycorrhizal fungus Glomus intraradices. FEMS Microbiol Ecol 41: 133–140CrossRefPubMedGoogle Scholar
  44. Marilley L, Aragno M (1999) Phylogenetic diversity of bacterial communities differing in degreee of proximity of Lolium perenne and Trifolium repens roots. Appl Soil Ecol 13: 127–136CrossRefGoogle Scholar
  45. Marschner P, Crowley DE, Higashi RM (1997) Root exudation and physiological status of a root-colonizing fluorescent pseudomonad in mycorrhizal and non-mycorrhizal pepper (Capsicum annuum L.). Plant Soil 189: 11–20CrossRefGoogle Scholar
  46. Marschner P, Crowley DE, Lieberei R (2001a) Arbuscular mycorrhizal infection changes the bacterial 16S rDNA community composition in the rhizosphere of maize. Mycorrhiza 11: 297–302CrossRefGoogle Scholar
  47. Marschner P, Yang CH, Lieberei R, Crowley DE (2001b) Soil and plant specific effects on bacterial community composition in the rhizosphere. Soil Biol Biochem 33: 1437–1445CrossRefGoogle Scholar
  48. Marschner P, Solaiman Z, Rengel Z (2006) Rhizosphere properties of Poaceae genotypes under P-limiting conditions. Plant Soil 283: 11–24CrossRefGoogle Scholar
  49. Marschner P, Solaiman Z, Rengel Z (2007) Brassica genotypes differ in growth, phosphorus uptake and rhizosphere properties under P-limiting conditions. Soil Biol Biochem 39: 87–98CrossRefGoogle Scholar
  50. McLaughlin MJ, Alston AM (1986) The relative contribution of plant residues and fertiliser to the phosphorus nutrition of wheat in a pasture/cereal system. Aust J Soil Res 24: 517–526CrossRefGoogle Scholar
  51. McLaughlin MJ, Alston AM, Martin JK (1988) Phosphorus cycling in wheat-pasture rotations. II. The role of the microbial biomass in phosphorus cycling. Aust J Soil Res 26: 333–342CrossRefGoogle Scholar
  52. Meharg AA, Killham K (1995) Loss of exudates from the roots of perennial ryegrass inoculated with a range of microorganisms. Plant Soil 170: 345–349CrossRefGoogle Scholar
  53. Merbach W, Mirus E, Knof G, Remus R, Ruppel S, Russow R, Gransee A, Schulze J (1999) Release of carbon and nitrogen compounds by plant roots and their possible ecological importance. J Plant Nutr Soil Sci 162: 373–383CrossRefGoogle Scholar
  54. Miethling R, Wieland G, Backhaus H, Tebbe CC (2000) Variation of microbial rhizosphere communities in response to crop species, soil origin, and inoculation with Sinorhizobium meliloti L33. Microbiol Ecol 40: 43–56Google Scholar
  55. Oberson A, Friesen DK, Rao IM, Buehler S, Frossard E (2001) Phosphorus transformations in an oxisol under contrasting land-use systems: the role of the soil microbial biomass. Plant Soil 237: 197–201CrossRefGoogle Scholar
  56. Richardson AE (2001) Prospects for using soil microorganisms to improve the acquisition of phosphorus by plants. Aust J Plant Physiol 28: 897–906Google Scholar
  57. Richardson AE, Hadobas PA (1997) Soil isolates of Pseudomonas spp. that utilize inositol phosphates. Can J Microbiol 43: 509–516PubMedCrossRefGoogle Scholar
  58. Richardson AE, Hadobas PA, Hayes JE, O’Hara CP, Simpson RJ (2001) Utilisation of phosphorus by pasture plants supplied with myo-inositol hexaphosphate is enhanced by the presence of soil micro-organisms. Plant Soil 229: 47–56CrossRefGoogle Scholar
  59. Römheld V (1991) The role of phytosiderophores in acquisition of iron and other micronutrients in graminaceous species: an ecological approach. Plant Soil 130: 127–134CrossRefGoogle Scholar
  60. Sahin F, Cakmakci R, Kantar F (2004) Sugar beet and barley yields in relation to inoculation with N2 fixing and phosphate solubilizing bacteria. Plant Soil 265: 123–129CrossRefGoogle Scholar
  61. Schmidt W, Martin P, Omay SH, Bangerth F (1988) Influence of Azospirillum brasiliense on nodulation of legumes. In: Klingmüller W (ed), Azospirillum IV: Genetics, Physiology, Ecology. Springer, Berlin/Heidelberg/New York, pp 92–100Google Scholar
  62. Seeling B, Zasoski RJ (1993) Microbial effects in maintaining organic and inorganic solution phosphorus concentrations in a grassland topsoil. Plant Soil 148: 277–284CrossRefGoogle Scholar
  63. Smalla K, Wieland G, Buchner A, Zock A, Parzy J, Kaiser S, Roskot N, Heuer H, Berg G (2001) Bulk and rhizosphere soil bacterial communities studied by denaturing gradient gel electrophoresis: plant-dependent enrichment and seasonal shifts revealed. Appl Environ Microbiol 67: 4742–4751PubMedCrossRefGoogle Scholar
  64. Smernik RJ, Dougherty WJ (2007) Identification of phytate in phosphorus-31 nuclear magnetic resonance spectra: the need for spiking. Soil Sci Soc Am J 71: 1045–1050CrossRefGoogle Scholar
  65. Ström L, Owen AG, Godbold DL, Jones DL (2001) Organic acid behaviour in a calcareous soil: sorption reactions and biodegradation rates. Soil Biol Biochem 33: 2125–2133CrossRefGoogle Scholar
  66. Tarafdar JC, Jungk A (1987) Phosphatase activity in the rhizosphere and its relation to the depletion of soil organic phosphorus. Biol Fert Soils 3: 199–204CrossRefGoogle Scholar
  67. Toro M, Azcon R, Barea JM (1997) Improvement of arbuscular mycorrhiza development by inoculation of soil with phosphate-solubilizing rhizobacteria to improve rock phosphate bioavailability (32P) and nutrient cycling. Appl Environ Microbiol 63: 4408–4412PubMedGoogle Scholar
  68. Turner BL, Mahieu N, Condron LM (2003) The phosphorus composition of temperate pasture soils determined by NaOH-EDTA extraction and solution 31P NMR spectroscopy. Org Geochem 34: 1199–1210CrossRefGoogle Scholar
  69. Van Hees PAW, Jones DL, Godbold DL (2002) Biodegradation of low molecular weight organic acids in coniferous forest podzolic soils. Soil Biol Biochem 34: 1261–1272CrossRefGoogle Scholar
  70. Vrede K, Heldal M, Norland S, Bartbak G (2002) Elemental composition (C, N, P) and cell volume of exponentially growing and nutrient-limited bacterioplankton. Appl Environ Microbiol 68: 2965–2971PubMedCrossRefGoogle Scholar
  71. Wang D, Marschner P, Solaiman Z, Rengel Z (2007) Belowground interactions between intercropped wheat and Brassicas in acidic and alkaline soils. Soil Biol Biochem 39: 961–971CrossRefGoogle Scholar
  72. Whipps JM, Lynch JM (1983) Substrate flow and utilization in the rhizosphere of cereals. New Phytol 95: 605–623CrossRefGoogle Scholar
  73. Whitelaw MA, Harden TJ, Helyar KR (1999) Phosphate solubilization in solution culture by the soil fungus Penicillium radicum. Soil Biol Biochem 31: 655–665CrossRefGoogle Scholar
  74. Yadav RS, Tarafdar JC (2003) Phytase and phosphatase producing fungi in arid and semi-arid soils and their efficiency in hydrolyzing different organic P compounds. Soil Biol Biochem 35: 1–7CrossRefGoogle Scholar
  75. Yang CH, Crowley DE (2000) Rhizosphere microbial community structure in relation to root location and plant iron nutritional status. Appl Environ Microbiol 66: 345–351PubMedCrossRefGoogle Scholar

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© Springer Science + Business Media B.V 2008

Authors and Affiliations

  • Petra Marschner
    • 1
  1. 1.School of Earth and Environmental SciencesThe University of AdelaideAustralia

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