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A Wide-Range Integrative Expression Vector (CoMed) System for Yeasts

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Yeast Biotechnology: Diversity and Applications

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No single yeast-based platform exists which is optimal for every protein. It is advisable to assess several platform candidates in parallel for optimal expression characteristics in a given case. For this approach, a wide-range yeast vector has been established that can be targeted to the various yeast host strains. The vector is built up in a modular way. In its basic form, it contains conserved rDNA-derived segments for targeting. For heterologous gene expression control, it is equipped with a promoter that is functional in all yeast species tested so far. For selection, a range of dominant and auxotrophic selection markers can be employed. Examples are presented applying vector variants with dominant or auxotrophic selection markers to the comparative simultaneous integration and expression of single or multiple foreign genes in a range of yeast platforms.

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Author information

Authors and Affiliations

  1. PharmedArtis GmbH, Forckenbeckstr. 6, Aachen, 52074, Germany

    Gerd Gellissen

Authors
  1. Gerhard Steinborn
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  2. Gotthard Kunze
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  3. Gerd Gellissen
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Editor information

Editors and Affiliations

  1. Department of Microbiology, University of Delhi, South Campus, New Delhi-110021, India

    T. Satyanarayana

  2. Institute of Plant Genetics and Crop Plant Research, Leibniz Institute, Corenstrasse 3, Gatersleben, D-06466, Germany

    Gotthard Kunze

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Steinborn, G., Kunze, G., Gellissen, G. (2009). A Wide-Range Integrative Expression Vector (CoMed) System for Yeasts. In: Satyanarayana, T., Kunze, G. (eds) Yeast Biotechnology: Diversity and Applications. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8292-4_17

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