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Assimilatory Nitrate Reduction in Hansenula polymorpha

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Yeast Biotechnology: Diversity and Applications

In the last decade, the yeast Hansenula polymorpha (syn.: Pichia angusta) has become an excellent experimental model for genetic and molecular investigations of nitrate assimilation, a subject traditionally investigated in plants, filamentous fungi and bacteria. Among other advantages, H. polymorpha offers classical and molecular genetic tools, as well as the availability of genomic sequence data.

Assimilative nitrate metabolism in H. polymorpha has an enzymological layout that is similar to other fungal species, and undergoes nitrogen metabolite repression elicited by preferred nitrogen sources such as glutamine. Genes involved in nitrate assimilation are clustered and independently transcribed. The information that puzzles is the presence of two homologous, albeit different, transcriptional activators acting upon the nitrate cluster genes, as all other known fungal nitrate assimilatory pathways have only one activator of this family. Recent work enables a first outline of the interplay between these two activators to be depicted, and suggests that one of them plays a central role in chromatin remodelling within the cluster.

The information, which has recently emerged regarding complex post-translational down-regulatory mechanism acting upon the major nitrate transporter suggests that this protein plays a central role in the regulation of nitrate assimilation.

Nitrogen metabolite repression acting upon nitrate assimilative genes is also being investigated through the isolation and characterisation of H. polymorpha Nmr mutants. These studies have suggested that the repression mechanisms are mediated by several interacting factors in this organism, which are also believed to participate in nitrogen metabolite repression of other metabolic pathways. All these are involved in the utilisation of secondary nitrogen sources such as arginine, meth-ylamine, urea and asparagine.

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Authors and Affiliations

  1. Laboratorio di Genetica Microbica, DiSA, Università Politecnica delle Marche, Via Brecce Bianche, Ancona, 60131, Italy

    Beatrice Rossi & Enrico Berardi

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  1. Beatrice Rossi
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  2. Enrico Berardi
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Editors and Affiliations

  1. Department of Microbiology, University of Delhi, South Campus, New Delhi-110021, India

    T. Satyanarayana

  2. Institute of Plant Genetics and Crop Plant Research, Leibniz Institute, Corenstrasse 3, Gatersleben, D-06466, Germany

    Gotthard Kunze

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Rossi, B., Berardi, E. (2009). Assimilatory Nitrate Reduction in Hansenula polymorpha . In: Satyanarayana, T., Kunze, G. (eds) Yeast Biotechnology: Diversity and Applications. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8292-4_15

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