Rhizobium leguminosarum Modification of Its Lipopoly-Saccharide During Symbiotic Bacteroid Development

  • E. L. Kannenberg
  • T. Härtner
  • L. S. Forsberg
  • R. W. Carlson
Conference paper
Part of the Current Plant Science and Biotechnology in Agriculture book series (PSBA, volume 42)

During pea nodule development, rhizobial lipopolysaccharides (LPSs) undergo host-specific modifications, likely of critical importance to nodule development. Previously, we analyzed the LPSs from Rhizobium leguminosarum (Rl) 3,841 bacteroids and found modifications to the LPS O-chain and lipid A that result in more hydrophobic LPSs. Consequently, the bacteroid LPS partitions mostly into phenol rather than water during hot phenol/water extraction (see Figure 1; Kannenberg and Carlson, 2001).

Keywords

Phenol Carbohydrate Hydrocarbon Electrophoresis Polyacrylamide 

Copyright information

© Springer Science + Business Media B.V. 2008

Authors and Affiliations

  • E. L. Kannenberg
    • 1
    • 2
  • T. Härtner
    • 2
  • L. S. Forsberg
    • 1
  • R. W. Carlson
    • 1
  1. 1.Complex Carbohydrate Research CenterUniversity of GeorgiaAthensUSA
  2. 2.Department of Microbiology/BiotechnologyUniversity of TübingenTübingenGermany

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