Quantitative and specific monitoring of live microorganisms under laboratory and especially field conditions is still one of the unsolved basic problems facing agricultural (soil) microbiology. Without the use of genetic modification, tracing associative inoculant biofertiliser strains released into the environment is a demanding task because of the large number of species of native microorganisms present in the rhizosphere. “Colony immuno-blotting” is a promising tool for following the success or failure of the colonizing ability/survival of bacterial strains in the soil matrix because it detects live numbers of a target strain by combining the advantages of traditional dilution plating with specific immuno-detection. However until now, this technique has been mostly employed in molecular biology using pure cultures. Therefore, adjustments for testing samples originated from the complex soil environment are needed.
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Kecskés, M.L., Michel, E., Lauby, B., Rakotondrainibe, M., Palágyi, A., Kennedy, I.R. (2008). Detection and Enumeration of Plant Growth-Promoting Bacteria. In: Dakora, F.D., Chimphango, S.B.M., Valentine, A.J., Elmerich, C., Newton, W.E. (eds) Biological Nitrogen Fixation: Towards Poverty Alleviation through Sustainable Agriculture. Current Plant Science and Biotechnology in Agriculture, vol 42. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8252-8_22
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