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Developmental stage conversion: Insights and possibilities

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Opportunistic Infections: Toxoplasma, Sarcocystis, and Microsporidia

Part of the book series: World Class Parasites ((WCPA,volume 9))

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Abstract

Toxoplasma gondii infection is acquired by oral ingestion. After dissemination, tachyzoites differentiate into bradyzoites within cysts that remain latent. The ability of bradyzoites to transform back into tachyzoites is a central factor in the ability of T. gondii to reactivate and cause disease in immunosuppressed individuals. Both tachyzoites and bradyzoites develop in tissue culture. Recent advances in the genetic manipulation of T. gondii have expanded the molecular tools that can be applied to studies on bradyzoite differentiation. Evidence is accumulating that this differentiation event is stress mediated and may share common pathways with stress-induced differentiation events in other eukaryotic organisms. Bradyzoite differentiation defective mutants have been studied using microarray analysis, allowing identification of genes that may be coordinately regulated during this stage transition. Characterization of unique bradyzoite-specific structures, such as the cyst wall, and stage-specific metabolic pathways are other approaches being used to study developmental stage conversion.

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References

  • Ajioka, J.W. 1998. Toxoplasma gondii: ESTs and gene discovery. International Journal for Parasitology 28 1025–1031.

    Article  PubMed  CAS  Google Scholar 

  • —, J.C. Boothroyd, B.P. Brunk, A. Hehl, L. Hillier, I.D. Manger, M. Marra, G.C. Overton, D.S. Roos, K.L. Wan, R. Waterston, and L. D. Sibley. 1998. Gene discovery by EST sequencing in Toxoplasma gondii reveals sequences restricted to the Apicomplexa. Genome Research 8 18–28.

    PubMed  Google Scholar 

  • Aramini, J.J., C. Stephen, J.P. Dubey, C. Engelstoft, H. Schwantje, and C.S. Ribble. 1999. Potential contamination of drinking water with Toxoplasma gondii oocysts. Epidemiology and Infection 122 305–315.

    Article  PubMed  CAS  Google Scholar 

  • Bohne, W., U. Gross, D.J. Ferguson, and J. Heesemann. 1995. Cloning and characterization of a bradyzoite-specifically expressed gene (hsp30/bag1) of Toxoplasma gondii, related to genes encoding small heat-shock proteins of plants. Molecular Microbiology 16 1221–1230.

    Article  PubMed  CAS  Google Scholar 

  • —, J. Heesemann, and U. Gross. 1993. Induction of bradyzoite-specific Toxoplasma gondii antigens in gamma interferon-treated mouse macrophages. Infection and Immunity 61 1141–1145.

    PubMed  CAS  Google Scholar 

  • —, —, and —. 1994. Reduced replication of Toxoplasma gondii is necessary for induction of bradyzoite-specific antigens: a possible role for nitric oxide in triggering stage conversion. Infection and Immunity 62 1761–1767.

    Google Scholar 

  • —, C.A. Hunter, M.W. White, D.J. Ferguson, U. Gross, and D.S. Roos. 1998. Targeted disruption of the bradyzoite-specific gene BAG1 does not prevent tissue cyst formation in Toxoplasma gondii. Molecular and Biochemical Parasitology 92: 115–126.

    Google Scholar 

  • —, A. Wirsing, and U. Gross. 1997. Bradyzoite-specific gene expression in Toxoplasma gondii requires minimal genomic elements. Molecular and Biochemical Parasitology 85: 1347–1354.

    Google Scholar 

  • —, A. Hehl, L.J. Knoll, and I.D. Manger. 1998. The surface of Toxoplasma: more and less. International Journal of Parasitology 28: 3–9.

    Article  PubMed  Google Scholar 

  • Bowie, W.R., A.S. King, D.H. Werker, J.L. Isaac-Renton, A. Bell, S.B. Eng, and S.A. Marion. 1997. Outbreak of toxoplasmosis associated with municipal drinking water. The BC Toxoplasma Investigation Team. Lancet 350: 173–177.

    Article  PubMed  CAS  Google Scholar 

  • Cleary, M.D., U. Singh, I.J. Blader, J.L. Brewer, and J.C. Boothroyd. 2002. Toxoplasma gondii asexual development: identification of developmentally regulated genes and distinct patterns of gene expression. Eukaryotic Cell 1: 329–340.

    Article  PubMed  CAS  Google Scholar 

  • Coppin, A., F. Dzierszinski, S. Legrand, M. Mortuaire, D. Ferguson, and S. Tomavo. 2003. Developmentally regulated biosynthesis of carbohydrate and storage polysaccharide during differentiation and tissue cyst formation in Toxoplasma gondii. Biochimie 85: 353–361.

    Article  PubMed  CAS  Google Scholar 

  • De Champs, C., C. Imbert-Bernard, A. Belmeguenai, J. Ricard, H. Pelloux, E. Brambilla, and P. Ambroise-Thomas. 1997. Toxoplasma gondii: in vivo and in vitro cystogenesis of the virulent RH strain. Journal of Parasitology 83: 152–155.

    Article  PubMed  Google Scholar 

  • Denton, H., C.W. Roberts, J. Alexander, K.W. Thong, and G.H. Coombs. 1996. Enzymes of energy metabolism in the bradyzoites and tachyzoites of Toxoplasma gondii. FEMS Microbiology Letters 137: 103–108.

    Article  PubMed  CAS  Google Scholar 

  • Donald, R.G., J. Allocco, S.B. Singh, B. Nare, S.P. Salowe, J. Wiltsie, and P.A. Liberator. 2002, Toxoplasma gondii cyclic GMP-dependent kinase: chemotherapeutic targeting of an essential parasite protein kinase. Eukaryotic Cell 1: 317–328.

    Article  PubMed  CAS  Google Scholar 

  • —, and P.A. Liberator. 2002. Molecular characterization of a coccidian parasite cGMPdependent protein kinase. Molecular and Biochemical Parasitology 120: 165–175.

    Article  PubMed  CAS  Google Scholar 

  • —, and D.S. Roos. 1993. Stable molecular transformation of Toxoplasma gondii: a selectable dihydrofolate reductase-thymidylate synthase marker based on drug-resistance mutations in malaria. Proceedings from the National Academy of Sciences USA 90: 11703–11707.

    Article  CAS  Google Scholar 

  • Dubey, J.P. 1997. Bradyzoite-induced murine toxoplasmosis: stage conversion, pathogenesis, and tissue cyst formation in mice fed bradyzoites of different strains of Toxoplasma gondii [published erratum appears in Journal of Eukaryotic Microbiology 1998 May-Jun;45(3):367]. The Journal of Eukaryotic Microbiology 44: 592–602.

    PubMed  CAS  Google Scholar 

  • —, D.S. Lindsay, and C.A. Speer. 1998. Structures of Toxoplasma gondii tachyzoites, bradyzoites, and sporozoites and biology and development of tissue cysts. Clinical Microbiology Reviews 11: 267–299.

    PubMed  Google Scholar 

  • —, C.A. Speer, S.K. Shen, O.C. Kwok, and J.A. Blixt. 1997. Oocyst-induced murine toxoplasmosis: life cycle, pathogenicity, and stage conversion in mice fed Toxoplasma gondii oocysts. Journal of Parasitology 83: 870–882.

    Article  PubMed  Google Scholar 

  • Dzierszinski, F., M. Mortuaire, M.F. Cesbron-Delauw, and S. Tomavo. 2000. Targeted disruption of the glycosylphosphatidylinositol-anchored surface antigen SAGS gene in Toxoplasma gondii decreases host cell adhesion and drastically reduces virulence in mice. Molecular Microbiology 37: 574–582.

    Article  PubMed  CAS  Google Scholar 

  • —, —, N. Dendouga, O. Popescu, and S. Tomavo. 2001. Differential expression of two plant-like enolases with distinct enzymatic and antigenic properties during stage conversion of the protozoan parasite Toxoplasma gondii. Journal of Molecular Biology 309, 1017–1027.

    Article  PubMed  Google Scholar 

  • —, O. Popescu, C. Toursel, C. Slomianny, B. Yahiaoui, and S. Tomavo. 1999. The protozoan parasite Toxoplasma gondii expresses two functional plant-like glycolytic enzymes. Implications for evolutionary origin of apicomplexans. Journal of Biological Chemistry 274, 24888–24895.

    Article  PubMed  Google Scholar 

  • Ferguson, D.J., and S.F. Parmley. 2002. Toxoplasma gondii MAG1 protein expression. Trends in Parasitology 18: 482.

    Article  PubMed  Google Scholar 

  • —, —, and S. Tomavo. 2002. Evidence for nuclear localisation of two stage-specific isoenzymes of enolase in Toxoplasma gondii correlates with active parasite replication. International Journal of Parasitology 32: 1399–1410.

    Article  PubMed  Google Scholar 

  • —, and W.M. Hutchison. 1987. An ultrastructural study of the early development and tissue cyst formation of Toxoplasma gondii in the brains of mice. Parasitology Research 73: 483–491.

    Article  PubMed  Google Scholar 

  • Fortier, B., C. Coignard-Chatain, M. Soete, and J.F. Dubremetz. 1996. [Structure and biology of Toxoplasma gondii bradyzoites]. Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales 190: 385–394.

    PubMed  CAS  Google Scholar 

  • Fox, B.A., and D.J. Bzik. 2002. De novo pyrimidine biosynthesis is required for virulence of Toxoplasma gondii. Nature 415: 926–929.

    Article  PubMed  CAS  Google Scholar 

  • Grigg, M.E., J. Ganatra, J.C. Boothroyd, and T.P. Margolis. 2001. Unusual abundance of atypical strains associated with human ocular toxoplasmosis. The Journal of Infectious Diseases 184: 633–639.

    Article  PubMed  CAS  Google Scholar 

  • Gross, U. 1996. Toxoplasma gondii. Berlin: Springer.

    Google Scholar 

  • —, W. Bohne, C.G. Luder, R. Lugert, F. Seeber, C. Dittrich, F. Pohl, and D.J. Ferguson. 1996. Regulation of developmental differentiation in the protozoan parasite Toxoplasma gondii. Journal of Eukaryotic Microbiology 43: 114S–116S.

    Article  PubMed  Google Scholar 

  • —, H. Bormuth, C. Gaissmaier, C. Dittrich, V. Krenn, W. Bohne, and D.J. Ferguson. 1995. Monoclonal rat antibodies directed against Toxoplasma gondii suitable for studying tachyzoite-bradyzoite interconversion in vivo. Clinical and Diagnostic Laboratory Immunology 2: 542–548.

    PubMed  Google Scholar 

  • Gurnett, A.M., P.A. Liberator, P.M. Dulski, S.P. Salowe, R.G. Donald, J.W. Anderson, J. Wiltsie, C.A. Diaz, G. Harris, B. Chang, S.J. Darkin-Rattray, B. Nare, T. Crumley, P.S. Blum, A.S. Misura, T. Tamas, M.K. Sardana, J. Yuan, T. Biftu, and D.M. Schmatz. 2002. Purification and molecular characterization of cGMP-dependent protein kinase from Apicomplexan parasites. A novel chemotherapeutic target. Journal of Biological Chemistry 277: 15913–15922.

    Article  PubMed  CAS  Google Scholar 

  • Holpert, M., C.G. Luder, U. Gross, and W. Bohne. 2001. Bradyzoite-specific expression of a P-type ATPase in Toxoplasma gondii. Molecular and Biochemical Parasitology 112: 293–296.

    Article  PubMed  CAS  Google Scholar 

  • Jacobs, L., and J. Remington. 1960. The resistance of the encysted form of Toxoplasma gondii. Journal of Parasitology 46: 11–21.

    Article  PubMed  CAS  Google Scholar 

  • Jerome, M.E., J.R. Radke, W. Bohne, D.S. Roos, and M.W. White. 1998. Toxoplasma gondii bradyzoites form spontaneously during sporozoite-initiated development. Infection and Immunity 66: 4838–4844.

    PubMed  CAS  Google Scholar 

  • Jones, T.C., K.A. Bienz, and P. Erb. 1986. In vitro cultivation of Toxoplasma gondii cysts in astrocytes in the presence of gamma interferon. Infection and Immunity 51: 147–156.

    PubMed  CAS  Google Scholar 

  • Khan, F., J. Tang, C.L. Qin, and K. Kim. 2002. Cyclin-dependent kinase TPK2 is a critical cell cycle regulator in Toxoplasma gondii. Molecular Microbiology 45: 321–332.

    Article  PubMed  CAS  Google Scholar 

  • Kirkman, L.A., L.M. Weiss, and K. Kim. 2001. Cyclic nucleotide signaling in Toxoplasma gondii bradyzoite differentiation. Infection and Immunity 69: 148–153.

    Article  PubMed  CAS  Google Scholar 

  • Knoll, L.J., and J.C. Boothroyd. 1998a. Isolation of developmentally regulated genes from Toxoplasma gondii by a gene trap with the positive and negative selectable marker hypoxanthine-xanthine-guanine phosphoribosyltransferase. Molecular and Cellular Biology 18: 807–814.

    PubMed  CAS  Google Scholar 

  • —, and —. 1998b. Molecular biology’s lessons about Toxoplasma development: stage-specific homologs. Parasitology Today 14: 490–493.

    Google Scholar 

  • Lane, A., M. Soete, J.F. Dubremetz, and J.E. Smith. 1996. Toxoplasma gondii: appearance of specific markers during the development of tissue cysts in vitro. Parasitology Research 82: 340–346.

    Article  PubMed  CAS  Google Scholar 

  • Lecordier, L., C. Mercier, G. Torpier, B. Tourvieille, F. Darcy, J.L. Liu, P. Maes, A. Tartar, A. Capron, and M.F. Cesbron-Delauw. 1993. Molecular structure of a Toxoplasma gondii dense granule antigen (GRA 5) associated with the parasitophorous vacuole membrane. Molecular and Biochemical Parasitology 59: 143–153.

    Article  PubMed  CAS  Google Scholar 

  • Lekutis, C., D.J. Ferguson, M.E. Grigg, M. Camps, and J.C. Boothroyd. 2001. Surface antigens of Toxoplasma gondii: variations on a theme. International Journal of Parasitology 31: 1285–1292.

    Article  PubMed  CAS  Google Scholar 

  • Li, L., B.P. Brunk, J.C. Kissinger, D. Pape, K. Tang, R.H. Cole, J. Martin, T. Wylie, M. Dante, S.J. Fogarty, O.K. Howe, P. Liberator, C. Diaz, J. Anderson, M. White, M.E. Jerome, E.A. Johnson, J.A. Radke, C.J. Stoeckert, Jr., R.H. Waterston, S.W. Clifton, D.S. Roos, and L.D. Sibley. 2003. Gene discovery in the apicomplexa as revealed by EST sequencing and assembly of a comparative gene database. Genome Research 13: 443–454.

    Article  PubMed  Google Scholar 

  • Lindsay, D.S., J.P. Dubey, B.L. Blagburn, and M. Toivio-Kinnucan. 1991. Examination of tissue cyst formation by Toxoplasma gondii in cell cultures using bradyzoites, tachyzoites, and sporozoites. Journal of Parasitology 77: 126–132.

    Article  PubMed  CAS  Google Scholar 

  • —, R.R. Mitschler, M.A. Toivio-Kinnucan, S.J. Upton, J.P. Dubey, and B.L. Blagburn. 1993a. Association of host cell mitochondria with developing Toxoplasma gondii tissue cysts. American Journal of Veterinary Research 54: 1663–1667.

    PubMed  CAS  Google Scholar 

  • —, M.A. Toivio-Kinnucan, and B.L. Blagburn. 1993b. Ultrastructural determination of cystogenesis by various Toxoplasma gondii isolates in cell culture. Journal of Parasitology 79: 289–292.

    Article  PubMed  CAS  Google Scholar 

  • Luft, B.J., and J.S. Remington. 1988. AIDS commentary. Toxoplasmic encephalitis. Journal of Infectious Diseases 157: 1–6.

    PubMed  CAS  Google Scholar 

  • Lyons, R.E., and A.M. Johnson. 1995. Heat shock proteins of Toxoplasma gondii. Parasite Immunology 17: 353–359.

    Article  PubMed  CAS  Google Scholar 

  • —, and —. 1998. Gene sequence and transcription differences in 70 kDa heat shock protein correlate with murine virulence of Toxoplasma gondii. International Journal for Parasitology 28: 1041–1051.

    Google Scholar 

  • Maeda, T., T. Saito, Y. Oguchi, M. Nakazawa, T. Takeuchi, and T. Asai. 2003. Expression and characterization of recombinant pyruvate kinase from Toxoplasma gondii tachyzoites. Parasitology Research 89: 259–265.

    PubMed  Google Scholar 

  • Manger, I.D., S. Parmley, L.D. Sibley, M. Marra, L. Hillier, R. Waterston, and J.C. Boothroyd. 1998a. Expressed sequence tag analysis of the bradyzoite stage of Toxoplasma gondii: identification of developmentally regulated genes. Infection and Immunity 66: 1632–1637.

    PubMed  CAS  Google Scholar 

  • —, —, and J.C. Boothroyd. 1998b. The surface of Toxoplasma tachyzoites is dominated by a family of glycosylphosphatidylinositol-anchored antigens related to SAG1. Infection and Immunity 66: 2237–2244.

    PubMed  Google Scholar 

  • Matrajt, M., R.G. Donald, U. Singh, and D.S. Roos. 2002. Identification and characterization of differentiation mutants in the protozoan parasite Toxoplasma gondii. Molecular Microbiology 44: 735–747.

    Article  PubMed  CAS  Google Scholar 

  • McHugh, T.D., A. Gbewonyo, J.D. Johnson, R.E. Holliman, and P.D. Butcher. 1993. Development of an in vitro model of Toxoplasma gondii cyst formation. FEMS Microbiology Letters 114: 325–332.

    Article  PubMed  CAS  Google Scholar 

  • McLeod, R., D. Mack, and C. Brown. 1991. Toxoplasma gondii—new advances in cellular and molecular biology. Experimental Parasitology 72: 109–121.

    Article  PubMed  CAS  Google Scholar 

  • Miller, C.M., N.C. Smith, and A.M. Johnson. 1999. Cytokines, nitric oxide, heat shock proteins and virulence in Toxoplasma. Parasitology Today 15: 418–422.

    Article  PubMed  CAS  Google Scholar 

  • Miller, M.A., I.A. Gardner, C. Kreuder, D.M. Paradies, K.R. Worcester, D.A. Jessup, E. Dodd, M.D. Harris, J.A. Ames, A.E. Packham, and P.A. Conrad. 2002. Coastal freshwater runoff is a risk factor for Toxoplasma gondii infection of southern sea otters (Enhydra lutris nereis). International Journal of Parasitology 32: 997–1006.

    Article  PubMed  CAS  Google Scholar 

  • Mineo, J.R., R. McLeod, D. Mack, J. Smith, I.A. Khan, K.H. Ely, and L.H. Kasper. 1993. Antibodies to Toxoplasma gondii major surface protein (SAG-1, P30) inhibit infection of host cells and are produced in murine intestine after peroral infection. Journal of Immunology 150: 3951–3964.

    CAS  Google Scholar 

  • Nare, B., J.J. Allocco, P.A. Liberator, and R.G. Donald. 2002. Evaluation of a cyclic GMP-dependent protein kinase inhibitor in treatment of murine toxoplasmosis: gamma interferon is required for efficacy. Antimicrobial Agents and Chemotherapy 46: 300–307.

    Article  PubMed  CAS  Google Scholar 

  • Odberg-Ferragut, C., M. Soete, A. Engels, B. Samyn, A. Loyens, J. Van Beeumen, D. Camus, and J.F. Dubremetz. 1996. Molecular cloning of the Toxoplasma gondii sag4 gene encoding an 18 kDa bradyzoite specific surface protein. Molecular and Biochemical Parasitology 82: 237–244.

    Article  PubMed  CAS  Google Scholar 

  • Parmley, S.F., L.M. Weiss, and S. Yang. 1995. Cloning of a bradyzoite-specific gene of Toxoplasma gondii encoding a cytoplasmic antigen. Molecular and Biochemical Parasitology 73: 253–257.

    Article  PubMed  CAS  Google Scholar 

  • —, S. Yang, G. Harth, L.D. Sibley, A. Sucharczuk, and J.S. Remington. 1994. Molecular characterization of a 65-kilodalton Toxoplasma gondii antigen expressed abundantly in the matrix of tissue cysts. Molecular and Biochemical Parasitology 66: 283–296.

    Article  PubMed  CAS  Google Scholar 

  • Popiel, I., M. Gold, and L. Choromanski. 1994. Tissue cyst formation of Toxoplasma gondii T-263 in cell culture. Journal of Eukaryotic Microbiology 41: 17S.

    Article  PubMed  CAS  Google Scholar 

  • —, —, and K.S. Booth. 1996. Quantification of Toxoplasma gondii bradyzoites. Journal of Parasitology 82: 330–332.

    Article  PubMed  Google Scholar 

  • Qin, C.L., J. Tang, and K. Kim. 1998. Cloning and in vitro expression of TPK3, a Toxoplasma gondii homologue of shaggy/glycogen synthase kinase-3 kinases. Molecular and Biochemical Parasitology 93: 273–283.

    Article  PubMed  CAS  Google Scholar 

  • Radke, J.R., M.N. Guerini, and M.W. White. 2003. A change in the premitotic period of the cell cycle is associated with bradyzoite differentiation in Toxoplasma gondii. Molecular and Biochemical Parasitology 131: 119–127.

    Article  PubMed  CAS  Google Scholar 

  • —, B. Striepen, M.N. Guerini, M.E. Jerome, D.S. Roos, and M.W. White. 2001. Defining the cell cycle for the tachyzoite stage of Toxoplasma gondii. Molecular and Biochemical Parasitology 115:, 165–175.

    Article  PubMed  CAS  Google Scholar 

  • Saito, T., T. Maeda, M. Nakazawa, T. Takeuchi, T. Nozaki, and T. Asai. 2002. Characterisation of hexokinase in Toxoplasma gondii tachyzoites. International Journal for Parasitology 32: 961–967.

    Article  PubMed  CAS  Google Scholar 

  • Scholytyseck, E., H. Mehlhorn, and B.E. Muller. 1974. Fine structure of cyst and cyst wall of Sarcocystis tenella, Besnoitia jellisoni, Frenkelia sp. and Toxoplasma gondii. Journal of Protozoology 21: 284–294.

    PubMed  CAS  Google Scholar 

  • Shiels, B., N. Aslam, S. McKellar, A. Smyth, and J. Kinnaird. 1997. Modulation of protein synthesis relative to DNA synthesis alters the timing of differentiation in the protozoan parasite Theileria annulata. Journal of Cell Science 110: 1441–1451.

    PubMed  CAS  Google Scholar 

  • Silva, N.M., R.T. Gazzinelli, D.A. Silva, E.A. Ferro, L.H. Kasper, and J.R. Mineo. 1998. Expression of Toxoplasma gondii-specific heat shock protein 70 during In vivo conversion of bradyzoites to tachyzoites. Infection and Immunity 66: 3959–3963.

    PubMed  CAS  Google Scholar 

  • Sims, T.A., J. Hay, and I.C. Talbot. 1989. An electron microscope and immunohistochemical study of the intracellular location of Toxoplasma tissue cysts within the brains of mice with congenital toxoplasmosis. British Journal of Experimental Pathology 70: 317–325.

    PubMed  CAS  Google Scholar 

  • Singh, U., J.L. Brewer, and J.C. Boothroyd. 2002. Genetic analysis of tachyzoite to bradyzoite differentiation mutants in Toxoplasma gondii reveals a hierarchy of gene induction. Molecular Microbiology 44: 721–733.

    Article  PubMed  CAS  Google Scholar 

  • Smith, J.E. 1993. Toxoplasmosis. Heidelberg: SpringeriVerlag.

    Google Scholar 

  • —, G. McNeil, Y.W. Zhang, S. Dutton, G. Biswas-Hughes, and P. Appleford. 1996. Serological recognition of Toxoplasma gondii cyst antigens. Current Topics in Microbiology and Immunology 219: 67–73.

    PubMed  Google Scholar 

  • Soderbom, F., and W.F. Loomis. 1998. Cell-cell signaling during Dictyostelium development. Trends in Microbiology 6: 402–406.

    Article  PubMed  CAS  Google Scholar 

  • Soete, M., D. Camus, and J.F. Dubremetz. 1994. Experimental induction of bradyzoite-specific antigen expression and cyst formation by the RH strain of Toxoplasma gondii in vitro. Experimental Parasitology 78: 361–370.

    Article  PubMed  CAS  Google Scholar 

  • —, and J.F. Dubremetz. 1996. Toxoplasma gondii: kinetics of stage-specific protein expression during tachyzoite bradyzoite conversion in vitro. Current Topics in Microbiology and Immunology 219: 76–80.

    Google Scholar 

  • —, B. Fortier, D. Camus, and J.F. Dubremetz. 1993. Toxoplasma gondii: kinetics of bradyzoite-tachyzoite interconversion in vitro. Experimental Parasitology 76: 259–264.

    Article  PubMed  CAS  Google Scholar 

  • Stwora-Wojczyk, M., J. Kissinger, S. Spitalnik, and B. Wojczyk. 2004. Identification and analysis of a family of Toxoplasma gondii UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferases. International Journal for Parasitology In press.

    Google Scholar 

  • Thomason, P., D. Traynor, and R. Kay. 1999. Taking the plunge. Terminal differentiation in Dictyostelium. Trends in Genetics 15: 15–19.

    Article  PubMed  CAS  Google Scholar 

  • Tomavo, S., and J.C. Boothroyd. 1995. Interconnection between organellar functions, development and drug resistance in the protozoan parasite, Toxoplasma gondii. International Journal of Parasitology 25:1293–1299.

    Article  PubMed  CAS  Google Scholar 

  • Weiss, L.M., and K. Kim. 2000. The development and biology of bradyzoites of Toxoplasma gondii. Frontiers in Bioscience 5: D391–405.

    Article  PubMed  CAS  Google Scholar 

  • —, D. Laplace, P. Takvorian, H.B. Tanowitz, and M. Wittner. 1996. The association of the stress response and Toxoplasma gondii bradyzoite development. Journal of Eukaryotic Microbiology 43: 120S.

    Article  PubMed  CAS  Google Scholar 

  • —, —, —, A. Cali, H.B. Tanowitz, and M. Wittner. 1994. Development of bradyzoites of Toxoplasma gondii in vitro. Journal of Eukaryotic Microbiology 41: 18S.

    Article  PubMed  Google Scholar 

  • —, —, —, —, A. Cali, and M. Wittner. 1995. A cell culture system for study of the development of Toxoplasma gondii bradyzoites. Journal of Eukaryotic Microbiology 42: 150–157.

    Article  PubMed  Google Scholar 

  • —, —, H.B. Tanowitz, and M. Wittner. 1992. Identification of Toxoplasma gondii bradyzoite-specific monoclonal antibodies. Journal of Infectious Diseases 166: 213–215.

    PubMed  Google Scholar 

  • —, Y.F. Ma, P.M. Takvorian, H.B. Tanowitz, and M. Wittner. 1998. Bradyzoite development in Toxoplasma gondii and the hsp70 stress response. Infection and Immunity 66: 3295–3302.

    PubMed  Google Scholar 

  • Wojczyk, B.S., F.K. Hagen, B. Striepen, H.C. Hang, C.R. Bertozzi, D.S. Roos, and S.L. Spitalnik. 2003. cDNA Cloning and Expression of UDP-N-acetyl-D-galactosamine: Polypeptide N-AcetylgalactosaminyltransferaseT1 from Toxoplasma gondii. Molecular and Biochemical Parasitology 131: 93–107.

    Article  PubMed  CAS  Google Scholar 

  • Wong, S.Y., and J.S. Remington. 1993. Biology of Toxoplasma gondii [editorial]. AIDS 7: 299–316.

    Article  PubMed  CAS  Google Scholar 

  • Yahiaoui, B., F. Dzierszinski, A. Bernigaud, C. Slomianny, D. Camus, and S. Tomavo. 1999. Isolation and characterization of a subtractive library enriched for developmentally regulated transcripts expressed during encystation of Toxoplasma gondii. Molecular and Biochemical Parasitology 99: 223–235.

    Article  PubMed  CAS  Google Scholar 

  • Yang, S., and S.F. Parmley. 1995. A bradyzoite stage-specifically expressed gene of Toxoplasma gondii encodes a polypeptide homologous to lactate dehydrogenase. Molecular and Biochemical Parasitology 73: 291–294.

    Article  PubMed  CAS  Google Scholar 

  • —, and —. 1997. Toxoplasma gondii expresses two distinct lactate dehydrogenase homologous genes during its life cycle in intermediate hosts. Gene 184: 1–12.

    Google Scholar 

  • Zhang, Y.W., S.K.. Halonen, Y.F. Ma, M. Wittner, and L.M. Weiss. 2001. Initial characterization of CST1, a Toxoplasma gondii cyst wall glycoprotein. Infection and Immunity 69: 501–507.

    Article  PubMed  CAS  Google Scholar 

  • —, K. Kim, Y.F. Ma, M. Wittner, H.B. Tanowitz, and L.M. Weiss. 1999. Disruption of the Toxoplasma gondii bradyzoite-specific gene BAG1 decreases in vivo cyst formation. Molecular Microbiology 31: 691–701.

    Article  PubMed  CAS  Google Scholar 

  • —, and J.E. Smith. 1995. Toxoplasma gondii: reactivity of murine sera against tachyzoite and cyst antigens via FAST-ELISA. International Journal for Parasitology 25: 637–640.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Medicine, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York, 10461, USA

    Kami Kim & Louis M. Weiss

  2. Department of Microbiology and Immunology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York, 10461, USA

    Kami Kim

  3. Department of Pathology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York, 10461, USA

    Louis M. Weiss

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  1. Kami Kim
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Editors and Affiliations

  1. Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg, Virginia, USA

    David S. Lindsay

  2. Albert Einstein College of Medicine, Bronx, New York, USA

    Louis M. Weiss

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© 2004 Kluwer Academic Publishers

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Kim, K., Weiss, L.M. (2004). Developmental stage conversion: Insights and possibilities. In: Lindsay, D.S., Weiss, L.M. (eds) Opportunistic Infections: Toxoplasma, Sarcocystis, and Microsporidia. World Class Parasites, vol 9. Springer, Boston, MA. https://doi.org/10.1007/978-1-4020-7846-0_6

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  • DOI: https://doi.org/10.1007/978-1-4020-7846-0_6

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4020-7814-9

  • Online ISBN: 978-1-4020-7846-0

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