Over 200 plant extracts were prepared from different family plants of rich flora of Turkey, in a frame of international collaborative project “ Bioactive Turkish Plant Constituents ” . The plants were chosen randomly and/or partly based on their folkloric uses which belonged to 40 distinct families growing in Aegean and Marmara region, and their extracts were screened for their DNA damaging activity as well as for their human ovarian cytotoxic activity.
For this purpose, firstly, methanol extracts of the collected plants were prepared and tested for their DNA damaging activity. Active extracts were diluted with water and partitioned with hexane, dichloromethane and ethyl acetate, successively, and the extracts were then tested for their activity. For DNA damaging activity, the extracts were tested against three yeasts RS321NYCp50 grown on galactose, RS321NpRAD52 on galactose, and RS321NpRAD52 on glucose, while A2780 human ovarian cell lines were used for cytotoxic activity tests. Among the all tested extracts, four most active extracts were selected for further studies, which are Ajuga postii, Laurus nobilis, Eucalyptus camaldulensis and Salvia hypargeia.
In this presentation, bioactivity-directed isolation and structure elucidation of the active constituents will be given. Structures of the constituents, which form namely terpenoids (sesqui, di- and triterpenoids) were based on spectroscopic techniques, particularly intensive NMR and Mass spectroscopies.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Verpoorte, R., 1998, Drug Discovery Today, 3, 232–238
Kinghorn, A.D. and Balandrin, M.F. (eds), 1992, ACS Symposium Series 534, Human Medicinal Agents from Plants, San Franscisco, CA, American Chemical Society, Washington, DC, pp 191–204
Akman, Y. and Ketenoğlu, O., 1986, The Climate and Vegetation of Turkey. In Proceedings of the Royal Society of Edinburgh, Section B, Biological Sciences, I.C. Hedge (ed), 89, pp 123–134
Davis, P.H., 1965–1985, Flora of Turkey and the East Aegean Islands, University Press, Edinburgh, Vols 1–9
Davis, P.H., Mill, R.R. and Tan, K. (eds), 1988, Flora of Turkey and the East Aegean Islands, (Supplement), University Press, Edinburgh, Vol 10
Güner, A., Özhatay, N., Ekim, T. and Başer, K.H.C. (eds), 2001, Flora of Turkey and the East Aegean Islands, (Supplement 2), University Press, Edinburgh, Vol 11
Baser, K.H.C., 2002, Pure Appl. Chem., 74 (4), 527–545
Johnson, R.K., Bartus, H.F., Hofmann, G.A., Bartus, J.O., Mong, S.-M., Faucette, L.F., McCabe, F.L., Chan, A. and Mirabelli, C.K., 1986, In Vitro and In Vivo Models for Detection of New Antitumor Drugs. In Organizing Committee of the 14th International Congress of Chemotherapy, L.J. Hanka, T.Kondo, and R. J. White (eds), Kyoto, Japan, pp 15 –26
Schwikkard, S., Zhou, B.N., Glass, T.E., Sharp, J.L., Mattern, M.R., Johnsson, R.K. and Kingston, D.G.I., 2000, J. Nat. Prod., 63, 457–460
GÖren, A.C., Zhou, B.N., Topcu, G., KÖkdil, G. and Kingston, D.G.I. 2005, Nat. Prod., Res. 19, 457–460
Topçu, G., KÖkdil, G., Türkmen, Z., Voelter, W., Adou, E. and Kingston, D.G.I., 2004, Zeitschrift f ür Naturforschung, 59b, 584–588
Bahçeevli, A.K., Kurucu, S., Kolak, U., Topçu, G., Adou, E. and Kingston, D.G.I., 2005, J. Nat. Prod., 68, 956–958
Topçu, G., 2006, J. Nat. Prod., 69, 482–487
Topçu, G. and Ulubelen, A., 1999, J. Nat. Prod., 62, 1605–1608
Topcu, G.,Türkmen, Z., Schilling, J.K. and Kingston, D.G.I., 2004, J. Nat. Prod., 67, 118–121
McBrien, K.D., Berry, R.L, Lowe, S.E., Neddermann, K.M., Bursuker, I., Huang, S., Klohr, S.E. and Leet, J.E., 1995, J. Antibiot., 48, 1446–1452
Topcu, G., Altiner, E.A., Gozcü, S., Halfon, B., Pezzuto, J.M. and Kingston, D.G.I., 2003, Planta Med., 69, 464–467
Ulubelen, A., Topçu, G., Chai, H.B. and Pezzuto, J.M., 1999, Pharm. Biol. 37, 148–151
Topcu, G., Türkmen, Z., Schilling, J.K., Kingston, D.G.I., Pezzuto, J.M. and Ulubelen, A., 2008. Pharm. Biol., 46, 180–184
Barla, A., Topçu, G., Öksüz, S., Tümen, G. and Kingston, D.G.I., 2007, Food Chem., 104, 1478–1484
Topcu, G., Turkmen, Z. Yapar, G. and Kingston, D.G.I., J. Agric. Food Chem. (submitted)
Kozuka, M., Sawada, T., Kasahara, F., Mizuta, E., Amano, T., Komiya, T. Goto, M., 1982, Chem. Pharm. Bull., 30, 1952–1963
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Springer Science + Business Media B.V
About this chapter
Cite this chapter
Topçu, G. et al. (2009). Bioactive Turkish Plant Extracts and Their Constituents. In: Şener, B. (eds) Innovations in Chemical Biology. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6955-0_6
Download citation
DOI: https://doi.org/10.1007/978-1-4020-6955-0_6
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-6954-3
Online ISBN: 978-1-4020-6955-0
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)