Advertisement

Determination of Ibuprofen, Pseudoephedrine HCl, Chlorpheniramine Maleate and Nipagen by Liquid Chromatography and Fractional Factorial Design

  • Bürge Aşçi Özlem
  • Aksu Dönmez
  • Abdürrezzak Bozdoğan
  • Sidika Sungur

In the present work, a rapid and sensitive gradient HPLC method was developed for the analysis of a new syrup preparation containing ibuprofen, pseu-doephedrine HCl, chlorpheniramine maleate and nipagen. The optimization process was carried out using 2 5−1 fractional factorial design. Five variables pH, flow rate and solvent ratios for three steps of gradient profile were regarded as factors in the optimisation. The resolution was used as analytical response. Using the optimised experimental conditions, the limits of quantitations (10σ/s) determined as 0.09, 0.01, 0.04 and 0.01 ppm for ibuprofen, pseudoephedrine HCl, chlorpheniramine maleate and nipagen, respectively.

The obtained results indicated that this procedure could be applied for the determination of ibuprofen, pseudoephedrine hydrochloride, chlorpheniramine maleate and nipagen in syrup preparations.

Keywords

Chlorpheniramine fractional factorial design RP-HPLC ibuprofen nipagen pseudoephedrine 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Montgomery, D.C., 1997, Design and Analysis of Experiments, 4th ed., Wiley, New YorkGoogle Scholar
  2. 2.
    Supelco, Chromatograpy, Products for Analysis and Purification, 2003–2004Google Scholar
  3. 3.
    Dong, Y.M., Chen, X.F. and Chen, Y.L., 2005,J. Pharm. Biomed. Anal., 39, 285–289PubMedCrossRefGoogle Scholar
  4. 4.
    Palabiyik, I.M., Dinç, E. and Onur, F., 2004, J. Pharm. Biomed. Anal., 34, 473–483PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science + Business Media B.V 2009

Authors and Affiliations

  • Bürge Aşçi Özlem
    • 1
  • Aksu Dönmez
    • 1
  • Abdürrezzak Bozdoğan
    • 1
  • Sidika Sungur
    • 1
  1. 1.Departmant of ChemistryYildiz Technical UniversityDavutpasaTurkey

Personalised recommendations