A locally isolated Gram negative bacterium designated as USMAA1020 was able to produce poly(3-hydroxybutyrate-co-4hydroxybutyrate) [P(3HB-co-4HB)] when grown on γbutyrolactone as the sole carbon source. The polyester was purified from freeze-dried cells and analyzed by nuclear magnetic resonance (NMR) spectroscopy. 1H and 13C NMR results confirmed the presence of 3HB and 4HB monomers. USMAA1020 are able to grow and produce 67 wt% P(3HB- co-8mol%4HB) of the dry cell weight in a one-step cultivation process using γ -butyrolactone as the sole carbon source. It accumulated P(3HB- co-4HB) copolyesters with higher 4HB fraction in nitrogen-free mineral medium containing various carbon sources such as γ-butyrolactone, 1,4-butanediol and 4-hydroxybu-tyric acid through a two-step cultivation process. The two-step cultivation process produced significantly higher 4HB fraction in the copolyester and the composition of copolyesters varied from 19 to 43 mol% 4HB, depending on the carbon sources supplied. Biochemical characterization, 16S rRNA sequence analysis, cellular fatty acids analysis and DNA base ratio determination revealed that strain USMAA1020 may represent a strain of a new species within the genus Cupriavidus.
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Abdullah, A.A., Yahya, A.R.M., Sudesh, K., Azizan, M.N.M., Majid, M.I.A. (2009). Microbial Synthesis of Poly (3-Hydroxybutyrate- co-4-Hydroxybutyrate) by Cupriavidus sp. USMAA1020 Isolated from Malaysian Environment. In: Şener, B. (eds) Innovations in Chemical Biology. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6955-0_18
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