A Finite-Difference Time-Domain Model of Optical Phase Contrast Microscope Imaging

  • Stoyan Tanev
  • James Pond
  • Paul Paddon
  • Valery V. Tuchin
Part of the NATO Science for Peace and Security Series book series (NAPSB)

The Finite-Difference Time-Domain (FDTD) modeling technique is applied to build a simple simulation equivalent of an optical phase contrast microscope. The model is validated by demonstrating the effect of optical immersion on the optical phase contrast microscope image of a simple biological cell containing a cytoplasm, a nucleus and a membrane. To the best of our knowledge, this is the first study using the FDTD approach to construct optical phase contrast microscope images. The results demonstrate the potential of the FDTD modelling approach and extend its area of applicability a new biomedical research area.


Biological cell finite-difference time-domain method optical phase contrast microscope optical clearing effect 


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Copyright information

© Springer Science+Business Media, B.V 2008

Authors and Affiliations

  • Stoyan Tanev
    • 1
  • James Pond
    • 2
  • Paul Paddon
    • 2
  • Valery V. Tuchin
    • 3
  1. 1.Department of Systems and Computer EngineeringCarleton UniversityOttawaCanada
  2. 2.Lumerical Solutions, Inc.VancouverCanada
  3. 3.Institute of Optics and BiophotonicsSaratov State UniversityRussia

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