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WLIP and Analogues of Tolaasin I, Lipodepsipeptides from Pseudomonas reactans and Pseudomonas tolaasii: A Comparison of Their Activity on Natural and Model Membranes

  • R. Paletti
  • M. Coraiola
  • A. Cimmino
  • P. Lo Cantore
  • A. Evidente
  • Nicola Sante Iacobellis
  • M. Dalla Serra

Tolaasin I and the White Line Inducing Principle (WLIP) are lipodepsipeptides (LDPs) produced by virulent strains of Pseudomonas tolaasii and P. reactans which are the causal agents of “brown blotch” disease of Agaricus bisporus and of the “yellowing” of Pleurotus ostreatus. Both tolaasin I and WLIP have haemolytic activity, permeabilize lipid vesicles and appear to be involved in mushroom tissue alterations by forming transmembrane pores. Herein, we describe the activity of tolaasin II and five natural tolaasin analogues on natural and model membranes. Tolaasin I and tolaasin II shared similar activity; the other tolaasin analogues were instead remarkably less active (up to 15 times when compared to tolaasin I) both on natural and model membranes. Additionally, the activity of mixtures formed by tolaasin I and WLIP on vesicles is reported. Preliminary evidences of an interaction of the two LDPs leading to an antagonistic action became apparent.

Keywords

Lipodepsipeptides Pseudomonas tolaasii Pseudomonas reactans liposomes haemolysis transmembrane pores 

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Copyright information

© Springer Science + Business Media, B.V 2008

Authors and Affiliations

  • R. Paletti
    • 1
  • M. Coraiola
    • 1
  • A. Cimmino
    • 2
  • P. Lo Cantore
    • 3
  • A. Evidente
    • 2
  • Nicola Sante Iacobellis
    • 3
  • M. Dalla Serra
    • 1
  1. 1.CNR-FBK Istituto di BiofisicaUnità di TrentoItaly
  2. 2.Dipartimento di Scienze del SuoloUniversità di NapoliItaly
  3. 3.Dipartimento di BiologiaUniversità della BasilicataItaly

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