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A Detailed Structural Model For The Eukaryotic Lhcii-Ps Ii Supercomplex

  • Jon Nield
  • James Barber
Conference paper

Abstract

High resolution photosystem II (PSII) core complex X-ray structures have so far been derived from preparations isolated from prokaryotic cyanobacteria. These have given significant information regarding PSII functionality, including water splitting, but as yet no high-resolution structure has become available for eukaryotic PSII. Here we use published X-ray structures of a cyanobacterial PSII core, LHCII, PsbP and PsbQ proteins to construct a high-resolution model for a eukaryotic LHCII-PSII supercomplex based upon a ̃17 Å resolution 3D density map determined by electron cryo-microscopy and single particle analysis. The positioning of chlorophylls was tentatively identified, as were the extrinsic proteins of the oxygen-evolving complex.

Keywords

Higher plants PSII structure electron microscopy 3D modelling water-splitting site 

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References

  1. Barber J (2003) Photosystem II: The engine of life. Quart Rev Biophys 36:71-89.CrossRefGoogle Scholar
  2. Calderone V, Trabucco M, Vujicic A, Battistutta R, Giacometti GM, Andreucci F, Barbato R, Zanotti G (2003) Crystal structure of the PsbQ protein of photosystem II from higher plants. EMBO reports 4:900-905.PubMedCrossRefGoogle Scholar
  3. Croce R, Canino G, Ros F, Bassi R (2002) Chromophore organization in the higher-plant photosystem II antenna protein CP26. Biochemistry 41:7334-7343.PubMedCrossRefGoogle Scholar
  4. De Las Rivas J, Barber J (2004) Analysis of the structure of the PsbO protein and its implications. Photosynth Res 81:329-343.CrossRefGoogle Scholar
  5. Ferreira KN, Iverson TM, Maghlaoui K, Barber J, Iwata S (2004) Architecture of the photosynthetic oxygenevolving center. Science 303:1831-1838.PubMedCrossRefGoogle Scholar
  6. Ifuku K, Nakatsu T, Kato H, Sato F (2004) Crystal structure of the PsbP protein of photosystem II from Nicotiana tabacum. EMBO reports 5:362-367.PubMedCrossRefGoogle Scholar
  7. Liu Z, Yan H, Wang K, Kuang T, Zhang J, Gui L, An X, Chang W (2004) Crystal structure of spinach major light-harvesting complex at 2.72 Å resolution. Nature 428:287-292.PubMedCrossRefGoogle Scholar
  8. Loll B, Kern J, Saenger W, Zouni A, Biesiadka J (2005) Towards complete cofactor arrangement in the 3.0 Å resolution structure of photosystem II. Nature 438:1040-1044.PubMedCrossRefGoogle Scholar
  9. Morosinotto T, Bassi R, Frigerio S, Finazzi G, Morris E, Barber J (2006) Biochemical and structural analyses of a higher plant photosystem II supercomplex of a photosystem I-less mutant of barley. Consequences of a chronic over-reduction of the plastoquinone pool. FEBS 273:4616-4630.CrossRefGoogle Scholar
  10. Nield J, Orlova EV, Morris EP, Gowen B, van Heel M, Barber J (2000) 3D map of the plant photosystem II supercomplex obtained by cryoelectron micros-copy and single particle analysis. Nature Struct Biol 7:44-47.PubMedCrossRefGoogle Scholar
  11. Nield J, Balsera M, De Las Rivas J, Barber J (2002) Three-dimensional electron cryo-microscopy study of the extrinsic domains of the oxygen-evolving complex of spinach: Assignment of the PsbO protein. J Biol Chem 277:15006-15012.PubMedCrossRefGoogle Scholar
  12. Rhee KH, Morris EP, Barber J, Kuhlbrandt W (1998) Three-dimensional structure of the plant photosystem II reac-tion centre at 8 Å resolution. Nature 396:283-286.PubMedCrossRefGoogle Scholar
  13. Sandona D, Croce R, Pagano A, Crimi M, Bassi R (1998) Higher plants light harvesting proteins. Structure and function as revealed by mutation analysis of either protein or chromophore moieties. Biochim Biophys Acta 1365:207-214.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science + Business Media, B.V. 2008

Authors and Affiliations

  • Jon Nield
    • 1
  • James Barber
    • 1
  1. 1.Wolfson Laboratories, Division of Molecular Biosciences, Faculty of Natural SciencesImperial College LondonLondonUK

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