A Detailed Structural Model For The Eukaryotic Lhcii-Ps Ii Supercomplex
High resolution photosystem II (PSII) core complex X-ray structures have so far been derived from preparations isolated from prokaryotic cyanobacteria. These have given significant information regarding PSII functionality, including water splitting, but as yet no high-resolution structure has become available for eukaryotic PSII. Here we use published X-ray structures of a cyanobacterial PSII core, LHCII, PsbP and PsbQ proteins to construct a high-resolution model for a eukaryotic LHCII-PSII supercomplex based upon a ̃17 Å resolution 3D density map determined by electron cryo-microscopy and single particle analysis. The positioning of chlorophylls was tentatively identified, as were the extrinsic proteins of the oxygen-evolving complex.
KeywordsHigher plants PSII structure electron microscopy 3D modelling water-splitting site
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