Effects of Hypergravity on the Chlorophyll Content and Growth of Root and Shoot During Development in Rice Plants

  • Pandit Vidyasagar
  • Sagar Jagtap
  • Amit Nirhali
  • Santosh Bhaskaran
  • Vishakha Hase
Conference paper

Abstract

Earlier Studies On Hypergravity Effects Showed Modification In The Metabolism Of Cell Wall Components, Promotion Of Metaxylem Development And Decrease In Extensibility Of Secondary Cell Walls In Arabidopsis Thaliana (Tamaoki Et Al. 2006; Nakabayashi Et Al. 2006). In The Present Study, The Effects Of Hypergravity On Rice Seeds Which Were Exposed To Hypergravity Conditions And Grown Under Normal Gravity Have Been Studied. Rice Seeds (Prh-10 Obtained From National Seeds Corporation, Govt. Of India) Were Suspended In Water In A Test Tube And Were Exposed To Hypergravity Ranging From 500–3,000 G For 10 Min. Seeds Exposed To Hypergravity Were Grown On 0.8% Agar Under Ambient Conditions And Light Intensity Of 1,250 Lux For 16 H Per Day. Seeds Unexposed To Hypergravity Grown Under The Same Conditions Acted As Control. Length Of Roots And Shoots Were Measured. Chlorophyll Was Extracted On The Fifth Day And Absorption And Fluorescence Spectra Were Recorded In Both Control And Hypergravity Samples. The Cross Parts Of The Roots Were Obtained And Studied Under The Microscope. The Results Obtained Showed That The Chlorophyll Content Was Less In The Samples Exposed To Hypergravity. The Roots Showed Changes In The Diameter Of Cells At The Core. To The Best Of Our Knowledge, Such Type Of Study Has Been Reported For The First Time.

Keywords

Hypergravity rice absorbance fluorescence chlorophyll content root structure 

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References

  1. Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physio l4 (1):1-15.CrossRefGoogle Scholar
  2. Hoson T, Nishitani K, Miyamoto K, Ueda J, Kamisaka S, Yamamoto R, Masuda Y (1996) Effects of hypergravity on growth and cell wall properties of cress hypocotyls. J Exp Bot 47(297):513-517.PubMedCrossRefGoogle Scholar
  3. Khasim SM (2002) Botanical Microtechnique: Principles and Practice. Capital, New Delhi, India.Google Scholar
  4. Koizumi T, Sakaki T, Usui S, Soga K, Wakabayashi K, Hoson T (2007) Changes in membrane lipid composition in azuki bean epicotyls under hypergravity conditions: Possible role of membrane sterols in gravity resistance. Adv Space Res 39:1198-1203.CrossRefGoogle Scholar
  5. Matsumoto S, Saito Y, Kumasaki S, Soga K, Wakabayashi K, Hoson T (2007) Up-regulation of expression of tubulin genes and roles of microtubules in hypergravity-induced growth modification in Arabidopsis hypocotyls. Adv Space Res 39:1176-1181.CrossRefGoogle Scholar
  6. Nakabayashi I, Karahara I, Tamaoki D, Masuda K, Wakasugi T, Yamada K, Soga K, Hoson T, Kamisaka S (2006) Hypergravity stimulus enhances primary xylem development and decreases mechanical properties of secondary cell walls in inflorescence stems of Arabidopsis thaliana. Ann Bot 97:1083-1090.PubMedCrossRefGoogle Scholar
  7. Pedroso MC, Durzan DJ (2000) Effects of different gravity environments on DNA fragmentation and cell death in Kalanchoe leaves. Ann Bot 86:983-994.PubMedCrossRefGoogle Scholar
  8. Pedroso MC, Magalhaes JR, Durzan DJ (2000) A nitric oxide burst precedes apoptosis in angiosperm and gymnosperm callus cells and foliar tissues. J Exp Bot 51 (347):1027-1036.PubMedCrossRefGoogle Scholar
  9. Soga K, Wakabayashik, Hoson T and Kamisaka S (1999) Hypergravity increases the molecular mass of xyloglucans by decreasing xyloglucan- degrading activity in azuki bean epicotyls. Plant Cell Physiol 40(6):581-585.PubMedCrossRefGoogle Scholar
  10. Soga K, Harada K, Wakayabashi K, Hoson T, Kamisaka S (1999) Increased molecular mass of hemicellulosic polysaccharides is involved in growth inhibition of maize coleoptiles and mesocotyls under hypergravity conditions. J Plant Res 112(1107):273-278.PubMedCrossRefGoogle Scholar
  11. Tamaoki D, Karahara I, Schreiber L, Wakasugi T, Yamada K, Kamisaka S (2006) Effects of hypergravity conditions on elongation growth and lignin formation in the inflorescence stem of Arabidopsis thaliana. J Plant Res 119: 79-84.PubMedCrossRefGoogle Scholar
  12. Wakabayashi K, Soga K, Kamisaka S, Hoson T (2005) Increase in the level of arabinoxylan-hydroxycinnamate network in cell walls of wheat coleoptiles grown under continuous hypergravity conditions. Physiol Plant 125(1):127-134.CrossRefGoogle Scholar
  13. Waldron KW, Brett CT (1990) Effects of extreme acceleration on the germination, growth and cell wall composition of pea epicotyls. J Exp Bot 41:71-77.CrossRefGoogle Scholar
  14. Yoshioka R, Soga K, Wakabayashi K, Takeba G, Hoson T (2003) Hypergravity-induced changes in gene expression in Arabidopsis hypocotyls. Adv Space Res 31 (10):2187-2193.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science + Business Media, B.V. 2008

Authors and Affiliations

  • Pandit Vidyasagar
    • 1
  • Sagar Jagtap
    • 1
  • Amit Nirhali
    • 1
  • Santosh Bhaskaran
    • 1
  • Vishakha Hase
    • 1
  1. 1.Biophysics Laboratory, Department of PhysicsUniversity of PunePuneIndia

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